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Literature summary extracted from

  • He, M.; Nie, Y.F.; Xu, P.
    A T42M substitution in bacterial 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) generates enzymes with increased resistance to glyphosate (2003), Biosci. Biotechnol. Biochem., 67, 1405-1409.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.19 expression of the chimeric mutant aroA-M1, comprising N-terminal part of the Escherichia coli enzyme and the C-terminal part of the enzyme from Salmonella typhimurium, and of T42M-aroA mutant in Escherichia coli strain BL21(DE3) Salmonella enterica subsp. enterica serovar Typhimurium
2.5.1.19 expression of the wild-type enzyme, of the chimeric mutant aroA-M1, comprising N-terminal part of the Escherichia coli enzyme and the C-terminal part of the enzyme from Salmonella typhimurium, and of T42M-aroA mutant in strain BL21(DE3) Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
2.5.1.19 D242A site-directed mutagenesis, the mutation is responsible for the high increase in activity Escherichia coli
2.5.1.19 D348A site-directed mutagenesis, the mutation is responsible for the high increase in activity Escherichia coli
2.5.1.19 additional information construction of chimeric mutants by fusing the N-terminal part of the Escherichia coli enzyme with the C-terminal part of the enzyme from Salmonella typhimurium, the chimeric mutant shows a 6fold lower Km for phosphoenolpyruvate, a 5fold higher Ki for glyphosate, and a 9fold higher specific activity than the wild-type enzyme from Escherichia coli Salmonella enterica subsp. enterica serovar Typhimurium
2.5.1.19 additional information construction of chimeric mutants by fusing the N-terminal part of the Escherichia coli enzyme with the C-terminal part of the enzyme from Salmonella typhimurium, the chimeric mutant shows a 6fold lower Km for phosphoenolpyruvate, a 5fold higher Ki for glyphosate, and a 9fold higher specific activity than the wild-type enzyme from Escherichia coli Escherichia coli
2.5.1.19 R100A site-directed mutagenesis, the mutation is responsible for the high increase in activity Escherichia coli
2.5.1.19 T42M site-directed mutagenesis, the mutation is responsible for the glyphosate resistance and the low Km for phosphoenolpyruvate Salmonella enterica subsp. enterica serovar Typhimurium
2.5.1.19 T42M site-directed mutagenesis, the mutation is responsible for the glyphosate resistance and the low Km for phosphoenolpyruvate Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.19 glyphosate competitive inhibition of the wild-type enzyme, mutant T42M is less sensitive Escherichia coli
2.5.1.19 glyphosate competitive inhibition of the wild-type enzyme, mutant T42M is less sensitive Salmonella enterica subsp. enterica serovar Typhimurium

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.19 additional information
-
 additional information kinetics, wild-type and mutant enzymes Salmonella enterica subsp. enterica serovar Typhimurium
2.5.1.19 additional information
-
 additional information kinetics, wild-type and mutant enzymes Escherichia coli
2.5.1.19 0.0018
-
 phosphoenolpyruvate pH 7.0, 30°C, mutant StaroA-T42M Salmonella enterica subsp. enterica serovar Typhimurium
2.5.1.19 0.0024
-
 phosphoenolpyruvate pH 7.0, 30°C, mutant EcaroA-T42M Escherichia coli
2.5.1.19 0.0223
-
 phosphoenolpyruvate pH 7.0, 30°C, wild-type EcaroA Escherichia coli
2.5.1.19 0.0436
-
 phosphoenolpyruvate pH 7.0, 30°C, wild-type StaroA Salmonella enterica subsp. enterica serovar Typhimurium

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.19 45000
-
 * 45000, wild-type and mutant enzyme, SDS-PAGE Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.19 phosphoenolpyruvate + 3-phosphoshikimate Salmonella enterica subsp. enterica serovar Typhimurium
-
 phosphate + 5-O-(1-carboxyvinyl)-3-phosphoshikimate
-
 ?
2.5.1.19 phosphoenolpyruvate + 3-phosphoshikimate Escherichia coli
-
 phosphate + 5-O-(1-carboxyvinyl)-3-phosphoshikimate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.19 Escherichia coli
-
-
-
2.5.1.19 Salmonella enterica subsp. enterica serovar Typhimurium
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.19 0.0058
-
 wild-type enzyme, cell exract Salmonella enterica subsp. enterica serovar Typhimurium
2.5.1.19 0.0076
-
 wild-type enzyme, cell exract Escherichia coli
2.5.1.19 0.0357
-
 mutant T42M, cell extract Escherichia coli
2.5.1.19 0.136
-
 mutant T42M, cell extract Salmonella enterica subsp. enterica serovar Typhimurium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.19 phosphoenolpyruvate + 3-phosphoshikimate
-
 Salmonella enterica subsp. enterica serovar Typhimurium phosphate + 5-O-(1-carboxyvinyl)-3-phosphoshikimate
-
 ?
2.5.1.19 phosphoenolpyruvate + 3-phosphoshikimate
-
 Escherichia coli phosphate + 5-O-(1-carboxyvinyl)-3-phosphoshikimate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.5.1.19 ? * 45000, wild-type and mutant enzyme, SDS-PAGE Escherichia coli

Synonyms

EC Number Synonyms Comment Organism
2.5.1.19 5-enolpyruvylshikimate-3-phosphate synthase
-
 Salmonella enterica subsp. enterica serovar Typhimurium
2.5.1.19 5-enolpyruvylshikimate-3-phosphate synthase
-
 Escherichia coli
2.5.1.19 EcaroA
-
 Escherichia coli
2.5.1.19 EPSPS
-
 Salmonella enterica subsp. enterica serovar Typhimurium
2.5.1.19 EPSPS
-
 Escherichia coli
2.5.1.19 StaroA
-
 Salmonella enterica subsp. enterica serovar Typhimurium

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.5.1.19 30
-
 assay at Salmonella enterica subsp. enterica serovar Typhimurium
2.5.1.19 30
-
 assay at Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.19 7
-
 assay at Salmonella enterica subsp. enterica serovar Typhimurium
2.5.1.19 7
-
 assay at Escherichia coli

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.5.1.19 0.0005
-
 glyphosate pH 7.0, 30°C, wild-type StaroA Salmonella enterica subsp. enterica serovar Typhimurium
2.5.1.19 0.0015
-
 glyphosate pH 7.0, 30°C, wild-type EcaroA Escherichia coli
2.5.1.19 0.0128
-
 glyphosate pH 7.0, 30°C, mutant StaroA-T42M Salmonella enterica subsp. enterica serovar Typhimurium
2.5.1.19 0.0305
-
 glyphosate pH 7.0, 30°C, mutant EcaroA-T42M Escherichia coli