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Literature summary extracted from

  • Relyea, H.A.; van der Donk, W.A.
    Mechanism and applications of phosphite dehydrogenase (2005), Bioorg. Chem., 33, 171-189.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.20.1.1 synthesis production of deuterium- or tritium-labeled substances, mutant enzymes could be applied as NADPH regeneration systems Pseudomonas stutzeri

Protein Variants

EC Number Protein Variants Comment Organism
1.20.1.1 E175A/A176R double mutant, 3.6-fold higher efficiency with NAD+, 1000-fold higher efficiency with NADP+, 3-fold favor for NADP+ over NAD+ as cofactor Pseudomonas stutzeri
1.20.1.1 H292F no activity, NAD+ binding is abolished Pseudomonas stutzeri
1.20.1.1 H292K no activity, NAD+ binding is abolished Pseudomonas stutzeri
1.20.1.1 H292N no activity, NAD+ binding is abolished Pseudomonas stutzeri
1.20.1.1 H292Q no activity, NAD+ binding is abolished Pseudomonas stutzeri
1.20.1.1 R237K strong increase in Km for both substrates, reduced Vmax Pseudomonas stutzeri

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.20.1.1 diethyl dicarbonate
-
 Pseudomonas stutzeri
1.20.1.1 sulfite strong inhibitor, competitive with respect to posphonate Pseudomonas stutzeri

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.20.1.1 phosphonate + H2O + NAD+ Alcaligenes faecalis NADH regeneration system phosphate + NADH
-
 ir
1.20.1.1 phosphonate + H2O + NAD+ Pseudomonas stutzeri NADH regeneration system phosphate + NADH
-
 ir
1.20.1.1 phosphonate + H2O + NAD+ Pseudomonas stutzeri WM88 NADH regeneration system phosphate + NADH
-
 ir

Organism

EC Number Organism UniProt Comment Textmining
1.20.1.1 Alcaligenes faecalis
-
-
-
1.20.1.1 Pseudomonas stutzeri
-
-
-
1.20.1.1 Pseudomonas stutzeri WM88
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
1.20.1.1 phosphonate + NAD+ + H2O = phosphate + NADH + H+ different reaction mechanism discussed Pseudomonas stutzeri
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.20.1.1 additional information enzyme does not accept any alternative substrates Pseudomonas stutzeri ?
-
 ?
1.20.1.1 additional information enzyme does not accept any alternative substrates Pseudomonas stutzeri WM88 ?
-
 ?
1.20.1.1 phosphonate + H2O + NAD+
-
 Alcaligenes faecalis phosphate + NADH
-
 ir
1.20.1.1 phosphonate + H2O + NAD+
-
 Pseudomonas stutzeri phosphate + NADH
-
 ir
1.20.1.1 phosphonate + H2O + NAD+ NADH regeneration system Alcaligenes faecalis phosphate + NADH
-
 ir
1.20.1.1 phosphonate + H2O + NAD+ NADH regeneration system Pseudomonas stutzeri phosphate + NADH
-
 ir
1.20.1.1 phosphonate + H2O + NAD+
-
 Pseudomonas stutzeri WM88 phosphate + NADH
-
 ir
1.20.1.1 phosphonate + H2O + NAD+ NADH regeneration system Pseudomonas stutzeri WM88 phosphate + NADH
-
 ir
1.20.1.1 phosphonate + H2O + NADP+ NADP+ is a very poor hydride acceptor with the wild type enzyme Pseudomonas stutzeri phosphate + NADPH
-
 ir
1.20.1.1 phosphonate + H2O + NADP+ NADP+ is a very poor hydride acceptor with the wild type enzyme Pseudomonas stutzeri WM88 phosphate + NADPH
-
 ir

Synonyms

EC Number Synonyms Comment Organism
1.20.1.1 PTDH
-
 Alcaligenes faecalis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.20.1.1 35
-
-
 Pseudomonas stutzeri

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.20.1.1 7.25
-
-
 Pseudomonas stutzeri

Cofactor

EC Number Cofactor Comment Organism Structure
1.20.1.1 NAD+
-
 Alcaligenes faecalis
1.20.1.1 NAD+
-
 Pseudomonas stutzeri
1.20.1.1 NADP+
-
 Pseudomonas stutzeri