BRENDA - Enzyme Database show

Arsenite oxidation by the heterotroph Hydrogenophaga sp. str. NT-14: the arsenite oxidase and its physiological electron acceptor

vanden Hoven, R.N.; Santini, J.M.; Biochim. Biophys. Acta 1656, 148-155 (2004)

Data extracted from this reference:

KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.20.2.1
0.035
-
arsenite
pH 5.5, temperature not specified in the publication
Hydrogenophaga sp.
1.20.99.1
0.035
-
arsenite
pH 5.5, calculated with an Mr of 309000 Da
Hydrogenophaga sp. NT14
1.20.99.1
0.061
-
arsenite
pH 5.5
Hydrogenophaga sp. NT-26
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.20.2.1
periplasm
-
Hydrogenophaga sp.
-
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
1.20.2.1
Iron
protein contains 10.6 mol/alpha3beta3 iron and 2.1 mol/alpha3beta3 molybdenum
Hydrogenophaga sp.
1.20.2.1
Molybdenum
protein contains 10.6 mol iron per alpha3beta3 unit and 2.1 mol molybdenum per alpha3beta3 molybdenum
Hydrogenophaga sp.
1.20.99.1
Iron
involved in catalysis, 10.6 mol per mol of alpha3beta3
Hydrogenophaga sp. NT14
1.20.99.1
Iron
involved in catalysis, enzyme contains a reduced molybdenum bis(molybdopterin) center in the larger subunit and a [3Fe4S] Rieske cluster in the smaller subunit
Hydrogenophaga sp. NT-26
1.20.99.1
Molybdenum
involved in catalysis, 2.1 mol per mol of alpha3beta3
Hydrogenophaga sp. NT14
1.20.99.1
Molybdenum
involved in catalysis, enzyme contains a reduced molybdenum bis(molybdopterin) center in the larger subunit and a [3Fe4S] Rieske cluster in the smaller subunit
Hydrogenophaga sp. NT-26
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.20.2.1
16000
-
3 * 86000, AroA, + 3 * 16000, AroB, alpha3beta3, SDS-PAGE
Hydrogenophaga sp.
1.20.2.1
86000
-
3 * 86000, AroA, + 3 * 16000, AroB, alpha3beta3, SDS-PAGE
Hydrogenophaga sp.
1.20.2.1
306000
-
gel filtration
Hydrogenophaga sp.
1.20.99.1
14000
-
2 * 98000, AroA, + 2 * 14000, AroB, alpha2beta2, SDS-PAGE
Hydrogenophaga sp. NT-26
1.20.99.1
16000
-
3 * 86000, AroA, + 3 * 16000, AroB, alpha3beta3, SDS-PAGE
Hydrogenophaga sp. NT14
1.20.99.1
86000
-
3 * 86000, AroA, + 3 * 16000, AroB, alpha3beta3, SDS-PAGE
Hydrogenophaga sp. NT14
1.20.99.1
98000
-
2 * 98000, AroA, + 2 * 14000, AroB, alpha2beta2, SDS-PAGE
Hydrogenophaga sp. NT-26
1.20.99.1
219000
-
gel filtration
Hydrogenophaga sp. NT-26
1.20.99.1
306000
-
gel filtration
Hydrogenophaga sp. NT14
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.20.2.1
arsenite + cytochrome c-551 + H2O
Hydrogenophaga sp.
the bacterium obtains energy from the reaction, required for chemolithoautotrophic growth
arsenate + reduced cytochrome c-551
-
-
?
1.20.2.1
arsenite + cytochrome c-551 + H2O
Hydrogenophaga sp. NT-14
the bacterium obtains energy from the reaction, required for chemolithoautotrophic growth
arsenate + reduced cytochrome c-551
-
-
?
1.20.99.1
arsenite + cytochrome c-551
Hydrogenophaga sp. NT14
the bacterium obtains energy from the reaction, required for chemolithoautotrophic growth
arsenate + reduced cytochrome c-551
-
-
?
1.20.99.1
arsenite + cytochrome c-551
Hydrogenophaga sp. NT-26
the bacterium obtains energy from the reaction, required for chemolithoautotrophic growth
arsenate + reduced cytochrome c-551
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.20.2.1
Hydrogenophaga sp.
-
-
-
1.20.2.1
Hydrogenophaga sp. NT-14
-
-
-
1.20.99.1
Hydrogenophaga sp. NT-26
-
-
-
1.20.99.1
Hydrogenophaga sp. NT14
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.20.2.1
-
Hydrogenophaga sp.
1.20.99.1
57.5fold to homogeneity from the periplasm by preparation of spheroplasts, ammonium sulfate fractionation, ultrafiltration, cation exchange chromatography, and gel filtration, purification via anion exchange or hydrophobic interaction chromatography is not successful
Hydrogenophaga sp. NT14
Reaction
EC Number
Reaction
Commentary
Organism
1.20.99.1
arsenite + acceptor = arsenate + reduced acceptor
electron transport chain, overview
Hydrogenophaga sp. NT14
1.20.99.1
arsenite + acceptor = arsenate + reduced acceptor
electron transport chain, overview
Hydrogenophaga sp. NT-26
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.20.2.1
5.6
-
pH 5.5, temperature not specified in the publication
Hydrogenophaga sp.
1.20.99.1
5.6
-
purified enzyme
Hydrogenophaga sp. NT14
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.20.2.1
arsenite + cytochrome c-551 + H2O
the bacterium obtains energy from the reaction, required for chemolithoautotrophic growth
658195
Hydrogenophaga sp.
arsenate + reduced cytochrome c-551
-
-
-
?
1.20.2.1
arsenite + cytochrome c-551 + H2O
the bacterium obtains energy from the reaction, required for chemolithoautotrophic growth
658195
Hydrogenophaga sp. NT-14
arsenate + reduced cytochrome c-551
-
-
-
?
1.20.2.1
arsenite + H2O + 2,6-dichlorophenolindophenol
-
658195
Hydrogenophaga sp.
arsenate + reduced 2,6-dichlorophenolindophenol
-
-
-
?
1.20.2.1
arsenite + H2O + 2,6-dichlorophenolindophenol
-
658195
Hydrogenophaga sp. NT-14
arsenate + reduced 2,6-dichlorophenolindophenol
-
-
-
?
1.20.99.1
arsenite + cytochrome c-551
the bacterium obtains energy from the reaction, required for chemolithoautotrophic growth
658195
Hydrogenophaga sp. NT14
arsenate + reduced cytochrome c-551
-
-
-
?
1.20.99.1
arsenite + cytochrome c-551
the bacterium obtains energy from the reaction, required for chemolithoautotrophic growth
658195
Hydrogenophaga sp. NT-26
arsenate + reduced cytochrome c-551
-
-
-
?
1.20.99.1
arsenite + cytochrome c-551
electron transport chain
658195
Hydrogenophaga sp. NT14
arsenate + cytochrome c-551
-
-
-
?
1.20.99.1
arsenite + cytochrome c-551
electron transport chain, overview
658195
Hydrogenophaga sp. NT-26
arsenate + cytochrome c-551
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
1.20.2.1
hexamer
3 * 86000, AroA, + 3 * 16000, AroB, alpha3beta3, SDS-PAGE
Hydrogenophaga sp.
1.20.99.1
hexamer
3 * 86000, AroA, + 3 * 16000, AroB, alpha3beta3, SDS-PAGE
Hydrogenophaga sp. NT14
1.20.99.1
tetramer
2 * 98000, AroA, + 2 * 14000, AroB, alpha2beta2, SDS-PAGE
Hydrogenophaga sp. NT-26
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.20.2.1
30.4
-
arsenite
pH 5.5, temperature not specified in the publication
Hydrogenophaga sp.
1.20.99.1
30.4
-
arsenite
pH 5.5, calculated with an Mr of 309000 Da
Hydrogenophaga sp. NT14
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.20.2.1
5.5
-
assay at
Hydrogenophaga sp.
1.20.99.1
5.5
-
-
Hydrogenophaga sp. NT14
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.20.2.1
cytochrome c-551
physiological electron acceptor for the arsenite oxidase
Hydrogenophaga sp.
1.20.99.1
cytochrome c-551
purified from Hydrogenophaga sp. strain NT-14, 11 kDa, probably a homodimer
Hydrogenophaga sp. NT14
1.20.99.1
cytochrome c-551
purified from Hydrogenophaga sp. strain NT-14, 11 kDa, probably a homodimer
Hydrogenophaga sp. NT-26
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.20.2.1
cytochrome c-551
physiological electron acceptor for the arsenite oxidase
Hydrogenophaga sp.
1.20.99.1
cytochrome c-551
purified from Hydrogenophaga sp. strain NT-14, 11 kDa, probably a homodimer
Hydrogenophaga sp. NT14
1.20.99.1
cytochrome c-551
purified from Hydrogenophaga sp. strain NT-14, 11 kDa, probably a homodimer
Hydrogenophaga sp. NT-26
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.20.2.1
0.035
-
arsenite
pH 5.5, temperature not specified in the publication
Hydrogenophaga sp.
1.20.99.1
0.035
-
arsenite
pH 5.5, calculated with an Mr of 309000 Da
Hydrogenophaga sp. NT14
1.20.99.1
0.061
-
arsenite
pH 5.5
Hydrogenophaga sp. NT-26
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.20.2.1
periplasm
-
Hydrogenophaga sp.
-
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
1.20.2.1
Iron
protein contains 10.6 mol/alpha3beta3 iron and 2.1 mol/alpha3beta3 molybdenum
Hydrogenophaga sp.
1.20.2.1
Molybdenum
protein contains 10.6 mol iron per alpha3beta3 unit and 2.1 mol molybdenum per alpha3beta3 molybdenum
Hydrogenophaga sp.
1.20.99.1
Iron
involved in catalysis, 10.6 mol per mol of alpha3beta3
Hydrogenophaga sp. NT14
1.20.99.1
Iron
involved in catalysis, enzyme contains a reduced molybdenum bis(molybdopterin) center in the larger subunit and a [3Fe4S] Rieske cluster in the smaller subunit
Hydrogenophaga sp. NT-26
1.20.99.1
Molybdenum
involved in catalysis, 2.1 mol per mol of alpha3beta3
Hydrogenophaga sp. NT14
1.20.99.1
Molybdenum
involved in catalysis, enzyme contains a reduced molybdenum bis(molybdopterin) center in the larger subunit and a [3Fe4S] Rieske cluster in the smaller subunit
Hydrogenophaga sp. NT-26
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.20.2.1
16000
-
3 * 86000, AroA, + 3 * 16000, AroB, alpha3beta3, SDS-PAGE
Hydrogenophaga sp.
1.20.2.1
86000
-
3 * 86000, AroA, + 3 * 16000, AroB, alpha3beta3, SDS-PAGE
Hydrogenophaga sp.
1.20.2.1
306000
-
gel filtration
Hydrogenophaga sp.
1.20.99.1
14000
-
2 * 98000, AroA, + 2 * 14000, AroB, alpha2beta2, SDS-PAGE
Hydrogenophaga sp. NT-26
1.20.99.1
16000
-
3 * 86000, AroA, + 3 * 16000, AroB, alpha3beta3, SDS-PAGE
Hydrogenophaga sp. NT14
1.20.99.1
86000
-
3 * 86000, AroA, + 3 * 16000, AroB, alpha3beta3, SDS-PAGE
Hydrogenophaga sp. NT14
1.20.99.1
98000
-
2 * 98000, AroA, + 2 * 14000, AroB, alpha2beta2, SDS-PAGE
Hydrogenophaga sp. NT-26
1.20.99.1
219000
-
gel filtration
Hydrogenophaga sp. NT-26
1.20.99.1
306000
-
gel filtration
Hydrogenophaga sp. NT14
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.20.2.1
arsenite + cytochrome c-551 + H2O
Hydrogenophaga sp.
the bacterium obtains energy from the reaction, required for chemolithoautotrophic growth
arsenate + reduced cytochrome c-551
-
-
?
1.20.2.1
arsenite + cytochrome c-551 + H2O
Hydrogenophaga sp. NT-14
the bacterium obtains energy from the reaction, required for chemolithoautotrophic growth
arsenate + reduced cytochrome c-551
-
-
?
1.20.99.1
arsenite + cytochrome c-551
Hydrogenophaga sp. NT14
the bacterium obtains energy from the reaction, required for chemolithoautotrophic growth
arsenate + reduced cytochrome c-551
-
-
?
1.20.99.1
arsenite + cytochrome c-551
Hydrogenophaga sp. NT-26
the bacterium obtains energy from the reaction, required for chemolithoautotrophic growth
arsenate + reduced cytochrome c-551
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.20.2.1
-
Hydrogenophaga sp.
1.20.99.1
57.5fold to homogeneity from the periplasm by preparation of spheroplasts, ammonium sulfate fractionation, ultrafiltration, cation exchange chromatography, and gel filtration, purification via anion exchange or hydrophobic interaction chromatography is not successful
Hydrogenophaga sp. NT14
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.20.2.1
5.6
-
pH 5.5, temperature not specified in the publication
Hydrogenophaga sp.
1.20.99.1
5.6
-
purified enzyme
Hydrogenophaga sp. NT14
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.20.2.1
arsenite + cytochrome c-551 + H2O
the bacterium obtains energy from the reaction, required for chemolithoautotrophic growth
658195
Hydrogenophaga sp.
arsenate + reduced cytochrome c-551
-
-
-
?
1.20.2.1
arsenite + cytochrome c-551 + H2O
the bacterium obtains energy from the reaction, required for chemolithoautotrophic growth
658195
Hydrogenophaga sp. NT-14
arsenate + reduced cytochrome c-551
-
-
-
?
1.20.2.1
arsenite + H2O + 2,6-dichlorophenolindophenol
-
658195
Hydrogenophaga sp.
arsenate + reduced 2,6-dichlorophenolindophenol
-
-
-
?
1.20.2.1
arsenite + H2O + 2,6-dichlorophenolindophenol
-
658195
Hydrogenophaga sp. NT-14
arsenate + reduced 2,6-dichlorophenolindophenol
-
-
-
?
1.20.99.1
arsenite + cytochrome c-551
the bacterium obtains energy from the reaction, required for chemolithoautotrophic growth
658195
Hydrogenophaga sp. NT14
arsenate + reduced cytochrome c-551
-
-
-
?
1.20.99.1
arsenite + cytochrome c-551
the bacterium obtains energy from the reaction, required for chemolithoautotrophic growth
658195
Hydrogenophaga sp. NT-26
arsenate + reduced cytochrome c-551
-
-
-
?
1.20.99.1
arsenite + cytochrome c-551
electron transport chain
658195
Hydrogenophaga sp. NT14
arsenate + cytochrome c-551
-
-
-
?
1.20.99.1
arsenite + cytochrome c-551
electron transport chain, overview
658195
Hydrogenophaga sp. NT-26
arsenate + cytochrome c-551
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.20.2.1
hexamer
3 * 86000, AroA, + 3 * 16000, AroB, alpha3beta3, SDS-PAGE
Hydrogenophaga sp.
1.20.99.1
hexamer
3 * 86000, AroA, + 3 * 16000, AroB, alpha3beta3, SDS-PAGE
Hydrogenophaga sp. NT14
1.20.99.1
tetramer
2 * 98000, AroA, + 2 * 14000, AroB, alpha2beta2, SDS-PAGE
Hydrogenophaga sp. NT-26
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.20.2.1
30.4
-
arsenite
pH 5.5, temperature not specified in the publication
Hydrogenophaga sp.
1.20.99.1
30.4
-
arsenite
pH 5.5, calculated with an Mr of 309000 Da
Hydrogenophaga sp. NT14
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.20.2.1
5.5
-
assay at
Hydrogenophaga sp.
1.20.99.1
5.5
-
-
Hydrogenophaga sp. NT14