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Literature summary extracted from

  • Hirasawa, M.; Rubio, L.M.; Griffin, J.L.; Flores, E.; Herrero, A.; Li, J.; Kim, S.K.; Hurley, J.K.; Tollin, G.; Knaff, D.B.
    Complex formation between ferredoxin and Synechococcus ferredoxin: nitrate oxidoreductase (2004), Biochim. Biophys. Acta, 1608, 155-162.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.7.7.2 expression of native and His-tagged nitrate reductase in Escherichia coli Synechococcus sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.7.7.2 KCl inhibition of nitrate reductase-ferredoxin complex formation at high ionic strength Synechococcus sp.
1.7.7.2 MgSO4 inhibition of nitrate reductase-ferredoxin complex formation at high ionic strength Synechococcus sp.
1.7.7.2 N-acetylsuccinimide 115fold excess, loss of more than 80% and 5-10% loss of ferredoxin-dependent and methylviologen dependent activity after 1 h, respectively Synechococcus sp.
1.7.7.2 NaCl more than 80% inhibition above 200 mM, inhibition of nitrate reductase-ferredoxin complex formation at high ionic strength Synechococcus sp.
1.7.7.2 Phenylglyoxal 76fold excess, approx. 60% and 20% loss of ferredoxin dependent and methylviologen dependent activity after 2 h, respectively Synechococcus sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.7.7.2 0.05
-
 oxidized ferredoxin spinach ferredoxin Synechococcus sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.7.7.2 Iron enzyme contains one iron sulfur cluster Synechococcus sp.
1.7.7.2 Molybdenum enzyme probably contains a bis-molybdopterin guanine dinucleotide Synechococcus sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.7.7.2 76000
-
 x * 76000, deduced from nucleotide sequence Synechococcus sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.7.7.2 nitrate + reduced ferredoxin Synechococcus sp.
-
 nitrite + H2O + oxidized ferredoxin
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.7.7.2 Synechococcus sp.
-
 PCC 7942
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.7.7.2 recombinant His-tagged nitrate reductase, Ni2+-affinity column Synechococcus sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.7.7.2 nitrate + reduced ferredoxin
-
 Synechococcus sp. nitrite + H2O + oxidized ferredoxin
-
 ?
1.7.7.2 nitrate + reduced ferredoxin spinach ferredoxin or Anabaena ferredoxin Synechococcus sp. nitrite + H2O + oxidized ferredoxin
-
 ?
1.7.7.2 nitrate + reduced methylviologen
-
 Synechococcus sp. nitrite + H2O + oxidized methylviologen
-
 ?
1.7.7.2 nitrite + H2O + oxidized ferredoxin
-
 Synechococcus sp. nitrate + reduced ferredoxin + H+
-
 r

Subunits

EC Number Subunits Comment Organism
1.7.7.2 ? x * 76000, deduced from nucleotide sequence Synechococcus sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.7.7.2 molybdopterin
-
 Synechococcus sp.