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Literature summary extracted from

  • Zadvorny, O.A.; Zorin, N.A.; Gogotov, I.N.; Gorlenko, V.M.
    Properties of stable hydrogenase from the purple sulfur bacterium Lamprobacter modestohalophilus (2004), Biochemistry, 69, 164-169.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.12.99.6 Cd2+ reversible inhibition Lamprobacter modestohalophilus
1.12.99.6 CO noncompetitive vs. oxidized methyl viologen, competitive vs. H2 Lamprobacter modestohalophilus
1.12.99.6 Mg2+ reversible inhibition Lamprobacter modestohalophilus
1.12.99.6 Ni2+ competitive vs. Oxidized methyl viologen Lamprobacter modestohalophilus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.12.99.6 soluble
-
Lamprobacter modestohalophilus
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.12.99.6 30000
-
1 * 64000 + 1 * 30000, SDS-PAGE Lamprobacter modestohalophilus
1.12.99.6 64000
-
1 * 64000 + 1 * 30000, SDS-PAGE Lamprobacter modestohalophilus

Organism

EC Number Organism UniProt Comment Textmining
1.12.99.6 Lamprobacter modestohalophilus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.12.99.6
-
Lamprobacter modestohalophilus

Storage Stability

EC Number Storage Stability Organism
1.12.99.6 20°C, 50 mM Tris-HCl, pH 7.0, 0.02% NaN3, storage under air, 50 d, approx. 80% loss of activity Lamprobacter modestohalophilus
1.12.99.6 20°C, 50 mM Tris-HCl, pH 7.0, 0.02% NaN3, storage under argon, 50 d, approx. 60% loss of activity Lamprobacter modestohalophilus
1.12.99.6 20°C, 50 mM Tris-HCl, pH 7.0, 0.02% NaN3, storage under H2, 50 d, approx. 70% loss of activity Lamprobacter modestohalophilus
1.12.99.6 4°C, 50 mM Tris-HCl, pH 7.0, 0.02% NaN3, storage under air, 60 d, approx. 40% loss of activity Lamprobacter modestohalophilus
1.12.99.6 4°C, 50 mM Tris-HCl, pH 7.0, 0.02% NaN3, storage under argon, 60 d, approx. 30% loss of activity Lamprobacter modestohalophilus
1.12.99.6 4°C, 50 mM Tris-HCl, pH 7.0, 0.02% NaN3, storage under H2, 60 d, approx. 50% loss of activity Lamprobacter modestohalophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.12.99.6 H2 + oxidized benzyl viologen
-
Lamprobacter modestohalophilus reduced benzyl viologen + H+
-
r
1.12.99.6 H2 + oxidized methyl viologen
-
Lamprobacter modestohalophilus reduced methyl viologen + H+
-
r

Subunits

EC Number Subunits Comment Organism
1.12.99.6 dimer 1 * 64000 + 1 * 30000, SDS-PAGE Lamprobacter modestohalophilus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.12.99.6 85
-
-
Lamprobacter modestohalophilus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.12.99.6 20 95 almost no activity at 20°C, approx. 20% of maximal activity at 95°C Lamprobacter modestohalophilus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.12.99.6 70
-
no loss of activity after 1.5 min at pH 7.0 Lamprobacter modestohalophilus
1.12.99.6 85
-
approx. 50% loss of activity after 1.5 min at pH 7.0 Lamprobacter modestohalophilus
1.12.99.6 100
-
almost complete loss of activity after 1.5 min at pH 7.0 Lamprobacter modestohalophilus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.12.99.6 4
-
H2 evolution Lamprobacter modestohalophilus
1.12.99.6 8.5 9.5 H2 oxidation Lamprobacter modestohalophilus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.12.99.6 0.007
-
CO 30°C, pH 7.0 Lamprobacter modestohalophilus
1.12.99.6 0.16
-
Ni2+ 30°C, pH 9.0 Lamprobacter modestohalophilus
1.12.99.6 1.35
-
Ni2+ 30°C, pH 7.0 Lamprobacter modestohalophilus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.12.99.6 Lamprobacter modestohalophilus isoelectric focusing
-
4.5