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Literature summary extracted from

  • Schofield, L.R.; Anderson, B.F.; Patchett, M.L.; Norris, G.E.; Jameson, G.B.; Parker, E.J.
    Substrate ambiguity and crystal structure of Pyrococcus furiosus 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase: an ancestral 3-deoxyald-2-ulosonate-phosphate synthase? (2005), Biochemistry, 44, 11950-11962.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.5.1.54 recombinant enzyme, in complex with phosphoenolpyruvate Pyrococcus furiosus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.54 additional information not inhibitory: phenylalanine, tyrosine, tryptophane Pyrococcus furiosus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.54 0.006
-
 2-deoxy-D-erythrose 4-phosphate pH 6.8, 60°C Pyrococcus furiosus
2.5.1.54 0.009
-
 D-erythrose 4-phosphate pH 6.8, 60°C Pyrococcus furiosus
2.5.1.54 0.033
-
 phosphoenolpyruvate pH 6.8, 60°C, cosubstrate 2-deoxy-D-erythrose 4-phosphate Pyrococcus furiosus
2.5.1.54 0.035
-
 phosphoenolpyruvate pH 6.8, 60°C, cosubstrate 2-deoxy-D-ribose 5-phosphate Pyrococcus furiosus
2.5.1.54 0.036
-
 phosphoenolpyruvate pH 6.8, 60°C, cosubstrate D-ribose 5-phosphate Pyrococcus furiosus
2.5.1.54 0.062
-
 phosphoenolpyruvate pH 6.8, 60°C, cosubstrate D-arabinose 5-phosphate Pyrococcus furiosus
2.5.1.54 0.093
-
 phosphoenolpyruvate pH 6.8, 60°C, cosubstrate D-erythrose 4-phosphate Pyrococcus furiosus
2.5.1.54 1.58
-
 D-ribose 5-phosphate pH 6.8, 60°C Pyrococcus furiosus
2.5.1.54 2.5
-
 2-deoxy-D-ribose 5-phosphate pH 6.8, 60°C Pyrococcus furiosus
2.5.1.54 2.7
-
 D-arabinose 5-phosphate pH 6.8, 60°C Pyrococcus furiosus

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.54 Pyrococcus furiosus Q8U0A9
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.54 additional information no substrate: D-glyceraldehyde 3-phosphate, D-glucose 6-phosphate Pyrococcus furiosus ?
-
 ?
2.5.1.54 phosphoenolpyruvate + 2-deoxy-D-erythrose 4-phosphate + H2O
-
 Pyrococcus furiosus 3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate + phosphate
-
 ?
2.5.1.54 phosphoenolpyruvate + 2-deoxy-D-ribose 5-phosphate + H2O
-
 Pyrococcus furiosus 3,5-dideoxy-D-gluco-octulosonate 8-phosphate + 3,5-dideoxy-D-manno-octulosonate 8-phosphate + phosphate
-
 ?
2.5.1.54 phosphoenolpyruvate + D-arabinose 5-phosphate
-
 Pyrococcus furiosus 3-deoxy-D-manno-octulosonate 8-phosphate + phosphate
-
 ?
2.5.1.54 phosphoenolpyruvate + D-erythrose 4-phosphate + H2O
-
 Pyrococcus furiosus 3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate + phosphate
-
 ?
2.5.1.54 phosphoenolpyruvate + D-ribose 5-phosphate + H2O
-
 Pyrococcus furiosus 3-deoxy-D-altro-octulosonate 8-phosphate + phosphate
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.5.1.54 1.12
-
 phosphoenolpyruvate pH 6.8, 60°C, cosubstrate D-arabinose 5-phosphate Pyrococcus furiosus
2.5.1.54 1.4
-
 phosphoenolpyruvate pH 6.8, 60°C, cosubstrate D-erythrose 4-phosphate Pyrococcus furiosus
2.5.1.54 1.7
-
 phosphoenolpyruvate pH 6.8, 60°C, cosubstrate 2-deoxy-D-ribose 5-phosphate Pyrococcus furiosus
2.5.1.54 2.5
-
 phosphoenolpyruvate pH 6.8, 60°C, cosubstrate D-ribose 5-phosphate Pyrococcus furiosus
2.5.1.54 3
-
 phosphoenolpyruvate pH 6.8, 60°C, cosubstrate 2-deoxy-D-erythrose 4-phosphate Pyrococcus furiosus