EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.5.1.58 | K294A | slightly higher peptide affinity than wild-type enzyme, 7fold decrease in affinity for farnesyl diphosphate, mutation decreases the positive charge in the diphosphate binding pocket and also decrease the value of Km(Mg2+), compared to wild-type, decrease in rate constant for farnesylation in absence of Mg2+ | Rattus norvegicus |
2.5.1.58 | K294Q | little alteration in peptide affinity, 2fold decrease in affinity for farnesyl diphosphate, mutation decreases the positive charge in the diphosphate binding pocket and also decrease the value of Km(Mg2+), compared to wild-type, decrease in rate constant for farnesylation in absence of Mg2+ | Rattus norvegicus |
2.5.1.58 | R291A | 2fold lower peptide affinity than wild-type enzyme, 3-4fold decrease in affinity for farnesyl diphosphate, mutation decreases the positive charge in the diphosphate binding pocket and also decrease the value of Km(Mg2+), compared to wild-type, decrease in rate constant for farnesylation in absence of Mg2+ | Rattus norvegicus |
2.5.1.58 | R291G | little alteration in peptide affinity, 3-4fold decrease in affinity for farnesyl diphosphate, mutation decreases the positive charge in the diphosphate binding pocket and also decrease the value of Km(Mg2+), compared to wild-type,decrease in rate constant for farnesylation in absence of Mg2+ | Rattus norvegicus |
2.5.1.58 | R291K | slightly higher peptide affinity than wild-type enzyme, mutation decreases the positive charge in the diphosphate binding pocket and also decrease the value of Km(Mg2+), compared to wild-type, decrease in rate constant for farnesylation in absence of Mg2+ | Rattus norvegicus |
2.5.1.58 | R291Q | little alteration in peptide affinity, mutation decreases the positive charge in the diphosphate binding pocket and also decrease the value of Km(Mg2+), compared to wild-type, decrease in rate constant for farnesylation in absence of Mg2+ | Rattus norvegicus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.5.1.58 | Rattus norvegicus | - |
recombinant enzyme | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.5.1.58 | farnesyl diphosphate + protein-cysteine | - |
Rattus norvegicus | S-farnesyl protein + diphosphate | - |
? |