EC Number | Cloned (Comment) | Organism |
---|---|---|
1.14.14.3 | gene luxAB, expression of wild-type and mutant enzymes in Escherichia coli strain BL21 | Vibrio harveyi |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.14.14.3 | A75G/C106V/V173A | site-directed mutagenesis, alpha-subunit residues, reduced activity compared to the wild-type enzyme, further red shift of emission spectrum | Vibrio harveyi |
1.14.14.3 | A75G/C106V/V173C | site-directed mutagenesis, alpha-subunit residues, reduced activity compared to the wild-type enzyme, further red shift of emission spectrum | Vibrio harveyi |
1.14.14.3 | A75G/C106V/V173S | site-directed mutagenesis, alpha-subunit residues, reduced activity compared to the wild-type enzyme, further red shift of emission spectrum | Vibrio harveyi |
1.14.14.3 | A75G/C106V/V173T | site-directed mutagenesis, alpha-subunit residues, reduced activity compared to the wild-type enzyme, further red shift of emission spectrum | Vibrio harveyi |
1.14.14.3 | additional information | substrate specificities of mutant enzymes and wild-type enzyme, overview, changes in the kinetics and emission spectrum on mutation of the chromophore-binding platform | Vibrio harveyi |
1.14.14.3 | V173A | site-directed mutagenesis, alpha-subunit residue, reduced activity and decreased stability of the C4a-hydroperoxyflavin intermediate compared to the wild-type enzyme, red shift of emission spectrum | Vibrio harveyi |
1.14.14.3 | V173C | site-directed mutagenesis, alpha-subunit residue, reduced activity and decreased stability of the C4a-hydroperoxyflavin intermediate compared to the wild-type enzyme, red shift of emission spectrum | Vibrio harveyi |
1.14.14.3 | V173F | site-directed mutagenesis, alpha-subunit residue, reduced activity and decreased stability of the C4a-hydroperoxyflavin intermediate compared to the wild-type enzyme, red shift of emission spectrum | Vibrio harveyi |
1.14.14.3 | V173H | site-directed mutagenesis, alpha-subunit residue, reduced activity and decreased stability of the C4a-hydroperoxyflavin intermediate compared to the wild-type enzyme, red shift of emission spectrum | Vibrio harveyi |
1.14.14.3 | V173I | site-directed mutagenesis, alpha-subunit residue, reduced activity and decreased stability of the C4a-hydroperoxyflavin intermediate compared to the wild-type enzyme, red shift of emission spectrum | Vibrio harveyi |
1.14.14.3 | V173L | site-directed mutagenesis, alpha-subunit residue, reduced activity and decreased stability of the C4a-hydroperoxyflavin intermediate compared to the wild-type enzyme, red shift of emission spectrum | Vibrio harveyi |
1.14.14.3 | V173N | site-directed mutagenesis, alpha-subunit residue, reduced activity and decreased stability of the C4a-hydroperoxyflavin intermediate compared to the wild-type enzyme, red shift of emission spectrum | Vibrio harveyi |
1.14.14.3 | V173S | site-directed mutagenesis, alpha-subunit residue, reduced activity and decreased stability of the C4a-hydroperoxyflavin intermediate compared to the wild-type enzyme, red shift of emission spectrum | Vibrio harveyi |
1.14.14.3 | V173T | site-directed mutagenesis, alpha-subunit residue, reduced activity and decreased stability of the C4a-hydroperoxyflavin intermediate compared to the wild-type enzyme, red shift of emission spectrum | Vibrio harveyi |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.14.14.3 | additional information | - |
additional information | affinity and dissociation constants for FMNH2 of wild-type and mutants enzymes, kinetics | Vibrio harveyi |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.14.3 | fatty aldehyde + FMNH2 + O2 | Vibrio harveyi | - |
fatty acid + FMN + H2O + hn | - |
ir | |
1.14.14.3 | additional information | Vibrio harveyi | luminescence pathway, overview | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.14.3 | Vibrio harveyi | - |
gene luxAB | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.14.14.3 | recombinant wild-type and mutant enzymes from Escherichia coli strain BL21 | Vibrio harveyi |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
1.14.14.3 | a long-chain aldehyde + FMNH2 + O2 = a long-chain fatty acid + FMN + H2O + hnu | residue Val173 of the alpha-subunit is essential for flavin binding and located in the active site, formation of a C4a-hydroperoxyflavin intermediate | Vibrio harveyi |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.14.14.3 | additional information | - |
luminescence activity and decay of wild-type and mutants with different aldehyde substrates | Vibrio harveyi |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.14.3 | decanal + FMNH2 + O2 | - |
Vibrio harveyi | decanoate + FMN + H2O + hn | - |
ir | |
1.14.14.3 | dodecanal + FMNH2 + O2 | - |
Vibrio harveyi | dodecanoate + FMN + H2O + hn | - |
ir | |
1.14.14.3 | fatty aldehyde + FMNH2 + O2 | - |
Vibrio harveyi | fatty acid + FMN + H2O + hn | - |
ir | |
1.14.14.3 | additional information | luminescence pathway, overview | Vibrio harveyi | ? | - |
? | |
1.14.14.3 | additional information | substrate specificities of mutant enzymes and wild-type enzyme, overview | Vibrio harveyi | ? | - |
? | |
1.14.14.3 | nonanal + FMNH2 + O2 | - |
Vibrio harveyi | nonanoate + FMN + H2O + hn | - |
ir | |
1.14.14.3 | octanal + FMNH2 + O2 | - |
Vibrio harveyi | octanoate + FMN + H2O + hn | - |
ir | |
1.14.14.3 | RCHO + FMNH2 + O2 | formation of a C4a-hydroperoxyflavin intermediate | Vibrio harveyi | RCOOH + FMN + H2O + hn | - |
ir | |
1.14.14.3 | undecanal + FMNH2 + O2 | - |
Vibrio harveyi | undecanoate + FMN + H2O + hn | - |
ir |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.14.14.3 | dimer | heterodimer | Vibrio harveyi |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.14.14.3 | bacterial luciferase | - |
Vibrio harveyi |
1.14.14.3 | luciferase | - |
Vibrio harveyi |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.14.14.3 | 23 | - |
assay at | Vibrio harveyi |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.14.14.3 | 7 | - |
assay at | Vibrio harveyi |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.14.14.3 | FMNH2 | enzyme is complexed with flavin mononucleotide, enzyme possesses a binding platform for the isoalloxazine ring of flavin, a chromophore whose 7,8-dimethyl benzene plane interacts with the isopropyl chain of alphaVal173, structure-fuction analysis | Vibrio harveyi |