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Electrochemical studies of arsenite oxidase: an unusual example of a highly cooperative two-electron molybdenum center

Hoke, K.R.; Cobb, N.; Armstrong, F.A.; Hille, R.; Biochemistry 43, 1667-1674 (2004)

Data extracted from this reference:

KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.20.99.1
additional information
-
additional information
electrochemical enzyme properties determination by noncatalytic and catalytic voltrammetry, temperature and pH-dependeny
Alcaligenes faecalis
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
1.20.99.1
Iron
dimeric enzyme contains a reduced molybdenum bis(molybdopterin) center in the larger subunit and a [3Fe4S] Rieske cluster in the smaller subunit
Alcaligenes faecalis
1.20.99.1
Molybdenum
dimeric enzyme contains a reduced molybdenum bis(molybdopterin) center in the larger subunit and a [3Fe4S] Rieske cluster in the smaller subunit, the molybdenoenzyme does not exhibit a Mo(V) EPR signal during oxidative-reductive titation, reversible reduction of the Mo(VI) center to Mo(IV), reduction potential and pH dependence determination
Alcaligenes faecalis
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.20.99.1
arsenite + acceptor
Alcaligenes faecalis
detoxification of arsenite to the less toxic arsenate
arsenate + reduced acceptor
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.20.99.1
Alcaligenes faecalis
-
-
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.20.99.1
arsenite + acceptor
-
658052
Alcaligenes faecalis
arsenate + reduced acceptor
-
-
-
?
1.20.99.1
arsenite + acceptor
detoxification of arsenite to the less toxic arsenate
658052
Alcaligenes faecalis
arsenate + reduced acceptor
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
1.20.99.1
dimer
alphabeta
Alcaligenes faecalis
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.20.99.1
Pterin molybdenum cofactor
dimeric enzyme contains a reduced molybdenum bis(molybdopterin) center in the larger subunit
Alcaligenes faecalis
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.20.99.1
Pterin molybdenum cofactor
dimeric enzyme contains a reduced molybdenum bis(molybdopterin) center in the larger subunit
Alcaligenes faecalis
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.20.99.1
additional information
-
additional information
electrochemical enzyme properties determination by noncatalytic and catalytic voltrammetry, temperature and pH-dependeny
Alcaligenes faecalis
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
1.20.99.1
Iron
dimeric enzyme contains a reduced molybdenum bis(molybdopterin) center in the larger subunit and a [3Fe4S] Rieske cluster in the smaller subunit
Alcaligenes faecalis
1.20.99.1
Molybdenum
dimeric enzyme contains a reduced molybdenum bis(molybdopterin) center in the larger subunit and a [3Fe4S] Rieske cluster in the smaller subunit, the molybdenoenzyme does not exhibit a Mo(V) EPR signal during oxidative-reductive titation, reversible reduction of the Mo(VI) center to Mo(IV), reduction potential and pH dependence determination
Alcaligenes faecalis
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.20.99.1
arsenite + acceptor
Alcaligenes faecalis
detoxification of arsenite to the less toxic arsenate
arsenate + reduced acceptor
-
-
?
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.20.99.1
arsenite + acceptor
-
658052
Alcaligenes faecalis
arsenate + reduced acceptor
-
-
-
?
1.20.99.1
arsenite + acceptor
detoxification of arsenite to the less toxic arsenate
658052
Alcaligenes faecalis
arsenate + reduced acceptor
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.20.99.1
dimer
alphabeta
Alcaligenes faecalis