Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Lei, B.; Ding, Q.; Tu, S.C.
    Identity of the emitter in the bacterial luciferase luminescence reaction: binding and fluorescence quantum yield studies of 5-decyl-4a-hydroxy-4a,5-dihydroriboflavin-5'-phosphate as a model (2004), Biochemistry, 43, 15975-15982.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.14.3 expression of wild-type and mutant C106A enzymes in Escherichia coli strain JM101 Vibrio harveyi

Protein Variants

EC Number Protein Variants Comment Organism
1.14.14.3 C106A site-directed mutagenesis, catalytic properties are similar to the wild-type enzyme, mutant shows 60% of wild-type quantum yield Vibrio harveyi

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.14.3 5-decyl-4a-hydroxy-4a,5-dihydroriboflavin-5'-phosphate binding and fluorescence quantum yield studies of the substance as a model, complexed with the enzyme in a 1:1 molcular ratio leading to 80% and 90% inhibition of wild-type and mutant C106A at 0.01 mM, respectively, binds to the active site Vibrio harveyi

Organism

EC Number Organism UniProt Comment Textmining
1.14.14.3 Vibrio harveyi
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.14.3 recombinant wild-type and mutant C106A enzymes from Escherichia coli strain JM101 to over 95% purity Vibrio harveyi

Reaction

EC Number Reaction Comment Organism Reaction ID
1.14.14.3 a long-chain aldehyde + FMNH2 + O2 = a long-chain fatty acid + FMN + H2O + hnu reaction mechanism and scheme, cofactor binding Vibrio harveyi
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.14.3 decanal + FMNH2 + O2
-
 Vibrio harveyi decanoate + FMN + H2O + hn
-
 ir

Synonyms

EC Number Synonyms Comment Organism
1.14.14.3 bacterial luciferase
-
 Vibrio harveyi

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.14.3 23
-
 assay at Vibrio harveyi

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.14.3 7
-
 assay at Vibrio harveyi

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.14.3 4a-hydroxy-4a,5-dihydroriboflavin-5'-phosphate model bioluminescence emitter molecule, binding and fluorescence quantum yield studies, complexed with the enzyme in a 1:1 molcular ratio Vibrio harveyi
1.14.14.3 FMNH2 physiological cofactor Vibrio harveyi