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Literature summary extracted from

  • Zhang, M.; White, T.A.; Schuermann, J.P.; Baban, B.A.; Becker, D.F.; Tanner, J.J.
    Structures of the Escherichia coli PutA proline dehydrogenase domain in complex with competitive inhibitors (2004), Biochemistry, 43, 12539-12548.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.5.5.2
-
 Escherichia coli

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.5.5.2 sitting-drop vaor diffusion method, structures of the PutA PRODH domain complexed with competitive inhibitors, acetate, L-lactate and L-tetrahydro-2-furoic acid Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
1.5.5.2 L432P mutant form of PutA86-669, 5fold decrease in turnover-number and a severe loss in thermostability Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.5.5.2 acetate competitive Escherichia coli
1.5.5.2 L-lactate competitive Escherichia coli
1.5.5.2 L-tetrahydro-2-furoic acid competitive Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.5.2 0.06
-
 L-proline 25°C, wild-type PutA86-669 Escherichia coli
1.5.5.2 0.13
-
 L-proline 25°C, L432P mutant form of PutA86-669 Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.5.5.2 76000
-
 gel electrophoresis Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.5.5.2 L-proline + acceptor + H2O Escherichia coli the enzyme catalyzes the first step of proline catabolism (S)-1-pyrroline-5-carboxylate + reduced acceptor
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.5.5.2 Escherichia coli P09546 PutA86-669
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.5.2
-
 Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.5.2 L-proline + acceptor + H2O the enzyme catalyzes the first step of proline catabolism Escherichia coli (S)-1-pyrroline-5-carboxylate + reduced acceptor
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.5.5.2 PRODH
-
 Escherichia coli
1.5.5.2 PutA proline dehydrogenase
-
 Escherichia coli

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.5.5.2 45
-
 50% loss of activity of wild-type PutA86-669 after 1 h, 50% loss of of L432P mutant form of PutA86-669 after 8 min Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.5.5.2 3
-
 L-proline 25°C, L432P mutant form of PutA86-669 Escherichia coli
1.5.5.2 17
-
 L-proline 25°C, wild-type PutA86-669 Escherichia coli

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.5.5.2 0.2
-
 L-tetrahydro-2-furoic acid
-
 Escherichia coli
1.5.5.2 1
-
 L-lactate
-
 Escherichia coli
1.5.5.2 30
-
 acetate
-
 Escherichia coli