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Literature summary extracted from

  • Vergis, J.M.; Beardsley, G.P.
    Catalytic mechanism of the cyclohydrolase activity of human aminoimidazole carboxamide ribonucleotide formyltransferase/inosine monophosphate cyclohydrolase (2004), Biochemistry, 43, 1184-1192.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.2.3 native and mutant proteins Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
2.1.2.3 additional information several site directed mutants created with mutations in the inosine monophosphate cyclohydrolase domain of the enzyme Homo sapiens
3.5.4.10 D125A 10fold increase in KM-value Homo sapiens
3.5.4.10 D125E 5fold increase in Km-value Homo sapiens
3.5.4.10 D125N 3fold increase in Km-value Homo sapiens
3.5.4.10 K137A 30fold increase in KM-value, 30fold decrease in kcat-value Homo sapiens
3.5.4.10 K137R 3fold increase in KM-value15fold decrease in kcat-value Homo sapiens
3.5.4.10 K66A more than 100fold increase in KM-value Homo sapiens
3.5.4.10 Y104A decrease in kcat-value by one orders of magnitude Homo sapiens
3.5.4.10 Y104A/D125A no enzymic activity Homo sapiens
3.5.4.10 Y104F decrease in kcat-value by two orders of magnitude Homo sapiens
3.5.4.10 Y104F/D125A no enzymice activity Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.4.10 0.0009
-
 5-Formamido-1-(5-phosphoribosyl)imidazole-4-carboxamide pH 7.5, 22°C, wild-type Homo sapiens
3.5.4.10 0.0027
-
 5-Formamido-1-(5-phosphoribosyl)imidazole-4-carboxamide pH 7.5, 22°C, mutant D125N Homo sapiens
3.5.4.10 0.0027
-
 5-Formamido-1-(5-phosphoribosyl)imidazole-4-carboxamide pH 7.5, 22°C, mutant K137R Homo sapiens
3.5.4.10 0.003
-
 5-Formamido-1-(5-phosphoribosyl)imidazole-4-carboxamide pH 7.5, 22°C, mutant Y104A Homo sapiens
3.5.4.10 0.0036
-
 5-Formamido-1-(5-phosphoribosyl)imidazole-4-carboxamide pH 7.5, 22°C, mutant Y104F Homo sapiens
3.5.4.10 0.0052
-
 5-Formamido-1-(5-phosphoribosyl)imidazole-4-carboxamide pH 7.5, 22°C, mutant D125E Homo sapiens
3.5.4.10 0.0091
-
 5-Formamido-1-(5-phosphoribosyl)imidazole-4-carboxamide pH 7.5, 22°C, mutant D125A Homo sapiens
3.5.4.10 0.031
-
 5-Formamido-1-(5-phosphoribosyl)imidazole-4-carboxamide pH 7.5, 22°C, mutant K137A Homo sapiens
3.5.4.10 0.115
-
 5-Formamido-1-(5-phosphoribosyl)imidazole-4-carboxamide pH 7.5, 22°C, mutant K66A Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.2.3 10-formyltetrahydrofolate + 5'-phosphoribosyl-5-amino-imidazole-4-carboxamide Homo sapiens penultimate step of the purine de novo synthesis pathway tetrahdrofolic acid + 5'-phosphoribosyl-5-formamido-4-imidazole-4-carboxamide
-
 ?
2.1.2.3 10-formyltetrahydrofolate + 5'-phosphoribosyl-5-amino-imidazole-4-carboxamide aves penultimate step of the purine de novo synthesis pathway tetrahydrofolic acid + 5'-phosphoribosyl-5-formamido-4-imidazole-4-carboxamide
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.2.3 aves
-
-
-
2.1.2.3 Homo sapiens
-
-
-
3.5.4.10 Homo sapiens
-
 bifunctional aminoimidazole carboxamide ribonucleotide formyltransferase/inosine monophosphate cyclohydrolase
-

Reaction

EC Number Reaction Comment Organism Reaction ID
2.1.2.3 10-formyltetrahydrofolate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide = tetrahydrofolate + 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide mechanism Homo sapiens
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.2.3 10-formyltetrahydrofolate + 5'-phosphoribosyl-5-amino-imidazole-4-carboxamide
-
 Homo sapiens tetrahdrofolic acid + 5'-phosphoribosyl-5-formamido-4-imidazole-4-carboxamide
-
 ?
2.1.2.3 10-formyltetrahydrofolate + 5'-phosphoribosyl-5-amino-imidazole-4-carboxamide penultimate step of the purine de novo synthesis pathway Homo sapiens tetrahdrofolic acid + 5'-phosphoribosyl-5-formamido-4-imidazole-4-carboxamide
-
 ?
2.1.2.3 10-formyltetrahydrofolate + 5'-phosphoribosyl-5-amino-imidazole-4-carboxamide
-
 aves tetrahydrofolic acid + 5'-phosphoribosyl-5-formamido-4-imidazole-4-carboxamide
-
 ?
2.1.2.3 10-formyltetrahydrofolate + 5'-phosphoribosyl-5-amino-imidazole-4-carboxamide penultimate step of the purine de novo synthesis pathway aves tetrahydrofolic acid + 5'-phosphoribosyl-5-formamido-4-imidazole-4-carboxamide
-
 ?
3.5.4.10 5-formamido-1-(5-phosphoribosyl)imidazole-4-carboxamide
-
 Homo sapiens IMP + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.2.3 dimer monomer-dimer equilibrium in solution, dimeric form is required for transformylase activity Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
2.1.2.3 AICAR transformylase/inosine monophosphate cyclohydrolase bifunctional enzyme catalyzing the last two steps of the purine de novo synthesis pathway Homo sapiens
2.1.2.3 AICAR transformylase/inosine monophosphate cyclohydrolase bifunctional enzyme catalyzing the last two steps of the purine de novo synthesis pathway aves
2.1.2.3 ATIC
-
 Homo sapiens
2.1.2.3 ATIC
-
 aves

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.5.4.10 0.014
-
 5-Formamido-1-(5-phosphoribosyl)imidazole-4-carboxamide pH 7.5, 22°C, mutantD125A Homo sapiens
3.5.4.10 0.036
-
 5-Formamido-1-(5-phosphoribosyl)imidazole-4-carboxamide pH 7.5, 22°C, mutantY104F Homo sapiens
3.5.4.10 0.062
-
 5-Formamido-1-(5-phosphoribosyl)imidazole-4-carboxamide pH 7.5, 22°C, mutantK66A Homo sapiens
3.5.4.10 0.136
-
 5-Formamido-1-(5-phosphoribosyl)imidazole-4-carboxamide pH 7.5, 22°C, mutantY104A Homo sapiens
3.5.4.10 0.147
-
 5-Formamido-1-(5-phosphoribosyl)imidazole-4-carboxamide pH 7.5, 22°C, mutant D125E Homo sapiens
3.5.4.10 0.276
-
 5-Formamido-1-(5-phosphoribosyl)imidazole-4-carboxamide pH 7.5, 22°C, mutantK137A Homo sapiens
3.5.4.10 0.507
-
 5-Formamido-1-(5-phosphoribosyl)imidazole-4-carboxamide pH 7.5, 22°C, mutant K137R Homo sapiens
3.5.4.10 0.603
-
 5-Formamido-1-(5-phosphoribosyl)imidazole-4-carboxamide pH 7.5, 22°C, mutant D125N Homo sapiens
3.5.4.10 8.6
-
 5-Formamido-1-(5-phosphoribosyl)imidazole-4-carboxamide pH 7.5, 22°C, wild-type Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
2.1.2.3 10-formyltetrahydrofolate
-
 Homo sapiens
2.1.2.3 10-formyltetrahydrofolate
-
 aves
2.1.2.3 additional information contains selenomethionine aves