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Literature summary extracted from

  • Bok, D.; Ruiz, A.; Yaron, O.; Jahng, W.J.; Ray, A.; Xue, L.; Rando, R.R.
    Purification and characterization of a transmembrane domain-deleted form of lecithin retinol acyltransferase (2003), Biochemistry, 42, 6090-6098.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.135 expression of a truncated enzyme form lacking the transmembrane N- and C-termini in Escherichia coli as His-tagged protein, which is insoluble in absence of detergents Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.135 additional information
-
 additional information steady-state kinetics Homo sapiens
2.3.1.135 0.00024
-
 all-trans-retinol
-
 Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.135 membrane integral Homo sapiens 16020
-
2.3.1.135 smooth endoplasmic reticulum
-
 Homo sapiens 5790
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.135 20000
-
 1 * 25000, full-length wild-type enzyme, SDS-PAGE, 1 * 20000, recombinant truncated enzyme, SDS-PAGE Homo sapiens
2.3.1.135 20000
-
 2 * 25000, full-length wild-type enzyme, SDS-PAGE, 2 * 20000, recombinant truncated enzyme, SDS-PAGE Homo sapiens
2.3.1.135 25000
-
 1 * 25000, full-length wild-type enzyme, SDS-PAGE, 1 * 20000, recombinant truncated enzyme, SDS-PAGE Homo sapiens
2.3.1.135 25000
-
 2 * 25000, full-length wild-type enzyme, SDS-PAGE, 2 * 20000, recombinant truncated enzyme, SDS-PAGE Homo sapiens
2.3.1.135 40000
-
 nonreducing PAGE, higher aggregation, up to pentamers, occurs in absence of denaturing agents Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.135 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.135 recombinant truncated enzyme from Escherichia coli in presence of 1% SDS, by nickel affinity chromatography to homogeneity Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.135 liver
-
 Homo sapiens
-
2.3.1.135 retina
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.135 phosphatidylcholine + 11-cis-retinol low activity Homo sapiens 2-acylglycerophosphocholine + 11-cis-retinyl acyl ester
-
 ?
2.3.1.135 phosphatidylcholine + all-trans-retinol
-
 Homo sapiens 2-acylglycerophosphocholine + all-trans-retinyl acylester
-
 ?
2.3.1.135 phosphatidylcholine + retinol-[cellular-retinol-binding-protein]
-
 Homo sapiens 2-acylglycerophosphocholine + retinyl-ester-[cellular-retinol-binding-protein]
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.135 dimer 2 * 25000, full-length wild-type enzyme, SDS-PAGE, 2 * 20000, recombinant truncated enzyme, SDS-PAGE Homo sapiens
2.3.1.135 monomer 1 * 25000, full-length wild-type enzyme, SDS-PAGE, 1 * 20000, recombinant truncated enzyme, SDS-PAGE Homo sapiens
2.3.1.135 More the enzyme exists as a mixture of monomer and dimer, determined by sedimentation equilibrium analysis and mass spectrometry, higher aggregation, up to pentamers, occurs in absence of denaturing agents Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
2.3.1.135 lecithin retinol acyltransferase
-
 Homo sapiens
2.3.1.135 LRAT
-
 Homo sapiens