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Literature summary extracted from

  • Beharry, Z.M.; Eby, D.M.; Coulter, E.D.; Viswanathan, R.; Neidle, E.L.; Phillips, R.S.; Kurtz, D.M., Jr.
    Histidine ligand protonation and redox potential in the Rieske dioxygenases: Role of a conserved aspartate in anthranilate 1,2-Dioxygenase (2003), Biochemistry, 42, 13625-13636.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.12.1 expression of mutant enzymes in Escherichia coli BL21-Codon Plus Acinetobacter sp.

Protein Variants

EC Number Protein Variants Comment Organism
1.14.12.1 D218A no activity Acinetobacter sp.
1.14.12.1 D218E no activity Acinetobacter sp.
1.14.12.1 D218N no activity Acinetobacter sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.12.1 24000
-
apha, beta, 3 * 52000 + 3 * 24000, SDS-PAGE, native mass by gel filtration Acinetobacter sp.
1.14.12.1 52000
-
apha, beta, 3 * 52000 + 3 * 24000, SDS-PAGE, native mass by gel filtration Acinetobacter sp.
1.14.12.1 220000
-
gel filtration, wild type and mutant enzymes Acinetobacter sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.12.1 anthranilic acid + NAD(P)H + O2 + H2O Acinetobacter sp. initial step in aerobic degradation of aromatic compounds, enables growth on anthranilate as sole carbon source catechol + CO2 + NAD(P)+ + NH3
-
?
1.14.12.1 anthranilic acid + NAD(P)H + O2 + H2O Acinetobacter sp. ADP1 initial step in aerobic degradation of aromatic compounds, enables growth on anthranilate as sole carbon source catechol + CO2 + NAD(P)+ + NH3
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.14.12.1 Acinetobacter sp.
-
-
-
1.14.12.1 Acinetobacter sp. ADP1
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.12.1 wild type and mutant enzymes Acinetobacter sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.12.1 anthranilic acid + NAD(P)H + O2 + H2O initial step in aerobic degradation of aromatic compounds, enables growth on anthranilate as sole carbon source Acinetobacter sp. catechol + CO2 + NAD(P)+ + NH3
-
?
1.14.12.1 anthranilic acid + NAD(P)H + O2 + H2O initial step in aerobic degradation of aromatic compounds, enables growth on anthranilate as sole carbon source Acinetobacter sp. ADP1 catechol + CO2 + NAD(P)+ + NH3
-
?
1.14.12.1 anthranilic acid + NADH + O2 + H2O
-
Acinetobacter sp. catechol + CO2 + NAD+ + NH3
-
?
1.14.12.1 anthranilic acid + NADH + O2 + H2O
-
Acinetobacter sp. ADP1 catechol + CO2 + NAD+ + NH3
-
?

Subunits

EC Number Subunits Comment Organism
1.14.12.1 hexamer apha, beta, 3 * 52000 + 3 * 24000, SDS-PAGE, native mass by gel filtration Acinetobacter sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.14.12.1 additional information
-
oxygenase component completely inactive at pH 9 Acinetobacter sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.12.1 iron-sulfur centre proximal Rieske [2Fe-2S] center Acinetobacter sp.
1.14.12.1 NADH
-
Acinetobacter sp.
1.14.12.1 NADPH
-
Acinetobacter sp.