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Literature summary extracted from

  • Guy, J.L.; Jackson, R.M.; Acharya, K.R.; Sturrock, E.D.; Hooper, N.M.; Turner, A.J.
    Angiotensin-converting enzyme-2 (ACE2): comparative modeling of the active site, specificity requirements, and chloride dependence (2003), Biochemistry, 42, 13185-13192.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.4.17.23 medicine potential important target in cardio-renal disease Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.17.23 expression of the mutant enzyme in CHO cells Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
3.4.17.23 additional information construction of a soluble truncated mutant enzyme lacking the transmembrane and cytosolic domains Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.17.23 Cl- inhibition is substrate dependent, inhibitory with substrate angiotensin II Homo sapiens
3.4.17.23 additional information no inhibition by lisinopril Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.17.23 0.005
-
 angiotensin II pH 7.4, 37°C Homo sapiens
3.4.17.23 0.0091
-
 angiotensin 3-8 pH 7.4, 37°C Homo sapiens
3.4.17.23 0.0126
-
 angiotensin 4-8 pH 7.4, 37°C Homo sapiens
3.4.17.23 0.0245
-
 angiotensin 5-8 pH 7.4, 37°C Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.17.23 Cl- binding ligands are Tyr207 and Arg514, possible model for chloride activation, effect is substrate dependent: activation with angiotensin I and (7-methoxycoumarin-4-yl)acetyl-APK(2,4-dinitrophenyl)-OH, inhibition with angiotensin II Homo sapiens
3.4.17.23 Zn2+ zinc-binding motif HEXXH and third zinc ligand glutamate402, zinc-binding protease Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.17.23 angiotensin I + H2O Homo sapiens
-
 angiotensin-(1-9) + Leu
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.17.23 Homo sapiens Q9BYF1
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.17.23 (7-methoxycoumarin-4-yl)acetyl-APK(2,4-dinitrophenyl)-OH + H2O synthetic fluorogenic substrate Homo sapiens (7-methoxycoumarin-4-yl)acetyl-AP + N6-(2,4-dinitrophenyl)-L-lysine
-
 ?
3.4.17.23 angiotensin I + H2O
-
 Homo sapiens angiotensin-(1-9) + Leu
-
 ?
3.4.17.23 angiotensin II + H2O
-
 Homo sapiens angiotensin-(1-7) + Phe
-
 ir
3.4.17.23 angiotensin-(3-8) + H2O
-
 Homo sapiens angiotensin-(3-7) + Phe
-
 ir
3.4.17.23 angiotensin-(4-8) + H2O
-
 Homo sapiens angiotensin-(4-7) + Phe
-
 ir
3.4.17.23 angiotensin-(5-8) + H2O
-
 Homo sapiens angiotensin-(5-7) + Phe
-
 ir

Synonyms

EC Number Synonyms Comment Organism
3.4.17.23 ACE2
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.17.23 37
-
 assay at Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.17.23 84
-
 angiotensin 4-8 pH 7.4, 37°C Homo sapiens
3.4.17.23 162
-
 angiotensin 3-8 pH 7.4, 37°C Homo sapiens
3.4.17.23 1110
-
 angiotensin II pH 7.4, 37°C Homo sapiens
3.4.17.23 1518
-
 angiotensin 5-8 pH 7.4, 37°C Homo sapiens