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Literature summary extracted from
- Novel heme-containing lyase, phenylacetaldoxime dehydratase from Bacillus sp. strain OxB-1: purification, characterization, and molecular cloning of the gene (2000), Biochemistry, 39, 800-809.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.8.1.4 | additional information | up to 5fold enhancement of activity with FMN under anaerobic conditions | Bacillus sp. (in: Bacteria) | |
| 4.8.1.4 | NaN3 | activation | Bacillus sp. (in: Bacteria) |  |
| 4.8.1.4 | SO32- | activation | Bacillus sp. (in: Bacteria) | |
| 4.8.1.4 | sulfite | substitutes for FMN, to a low degree | Bacillus sp. (in: Bacteria) | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 4.8.1.4 | 0.03 | - |
(Z)-phenylacetaldehyde oxime | pH.0, 30°C, aerobic conditions | Bacillus sp. (in: Bacteria) | |
| 4.8.1.4 | 0.031 | - |
(Z)-phenylacetaldehyde oxime | pH.0, 30°C, anaerobic conditions | Bacillus sp. (in: Bacteria) | |
| 4.8.1.4 | 0.31 | - |
(Z)-phenylacetaldehyde oxime | pH.0, 30°C, aerobic conditions, Na2SO3 substitutes for FMN | Bacillus sp. (in: Bacteria) | |
| 4.8.1.4 | 0.846 | - |
(Z)-naphthoacetaldoxime | pH 7.0, 30°C | Bacillus sp. (in: Bacteria) | |
| 4.8.1.4 | 0.872 | - |
(Z)-phenylacetaldehyde oxime | pH 7.0, 30°C | Bacillus sp. (in: Bacteria) | |
| 4.8.1.4 | 1.24 | - |
(Z)-p-chlorophenylacetaldoxime | pH 7.0, 30°C | Bacillus sp. (in: Bacteria) | |
| 4.8.1.4 | 1.36 | - |
(Z)-3-phenylpropionaldoxime | pH 7.0, 30°C | Bacillus sp. (in: Bacteria) | |
| 4.8.1.4 | 2.4 | - |
(E/Z)-indoleacetaldoxime | pH 7.0, 30°C | Bacillus sp. (in: Bacteria) | |
| 4.8.1.4 | 2.42 | - |
(E/Z)-n-valeraldoxime | pH 7.0, 30°C | Bacillus sp. (in: Bacteria) | |
| 4.8.1.4 | 2.98 | - |
(E/Z)-isocapronaldoxime | pH 7.0, 30°C | Bacillus sp. (in: Bacteria) | |
| 4.8.1.4 | 3.08 | - |
(Z)-p-methoxyphenylacetaldoxime | pH 7.0, 30°C | Bacillus sp. (in: Bacteria) | |
| 4.8.1.4 | 3.58 | - |
(E/Z)-isovaleraldoxime | pH 7.0, 30°C | Bacillus sp. (in: Bacteria) | |
| 4.8.1.4 | 4.32 | - |
(E/Z)-propionaldoxime | pH 7.0, 30°C | Bacillus sp. (in: Bacteria) | |
| 4.8.1.4 | 5.24 | - |
(E/Z)-4-phenylbutyraldoxime | pH 7.0, 30°C | Bacillus sp. (in: Bacteria) | |
| 4.8.1.4 | 6.12 | - |
(E/Z)-n-capronaldoxime | pH 7.0, 30°C | Bacillus sp. (in: Bacteria) | |
| 4.8.1.4 | 11.1 | - |
(E/Z)-n-butyraldoxime | pH 7.0, 30°C | Bacillus sp. (in: Bacteria) | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 4.8.1.4 | soluble | - |
Bacillus sp. (in: Bacteria) | - |
- |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.8.1.4 | Fe2+ | activation | Bacillus sp. (in: Bacteria) | |
| 4.8.1.4 | Sn2+ | activation | Bacillus sp. (in: Bacteria) | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 4.8.1.4 | 40000 | - |
native PAGE | Bacillus sp. (in: Bacteria) |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 4.8.1.4 | Bacillus sp. (in: Bacteria) | - |
- |
- |
| 4.8.1.4 | Bacillus sp. (in: Bacteria) OxB-1 | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 4.8.1.4 | - |
Bacillus sp. (in: Bacteria) |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.8.1.4 | (E/Z)-4-phenylbutyraldoxime | 6.8% of activity with (Z)-phenylacetaldehyde oxime | Bacillus sp. (in: Bacteria) | phenylbutyronitrile + H2O | - |
? | |
| 4.8.1.4 | (E/Z)-4-phenylbutyraldoxime | 6.8% of activity with (Z)-phenylacetaldehyde oxime | Bacillus sp. (in: Bacteria) OxB-1 | phenylbutyronitrile + H2O | - |
? | |
| 4.8.1.4 | (E/Z)-indoleacetaldoxime | 18.1% of activity with (Z)-phenylacetaldehyde oxime | Bacillus sp. (in: Bacteria) | indoleacetonitrile + H2O | - |
? | |
| 4.8.1.4 | (E/Z)-indoleacetaldoxime | 18.1% of activity with (Z)-phenylacetaldehyde oxime | Bacillus sp. (in: Bacteria) OxB-1 | indoleacetonitrile + H2O | - |
? | |
| 4.8.1.4 | (E/Z)-isocapronaldoxime | 29.4% of activity with (Z)-phenylacetaldehyde oxime | Bacillus sp. (in: Bacteria) | isocapronitrile + H2O | - |
? | |
| 4.8.1.4 | (E/Z)-isovaleraldoxime | 20.2% of activity with (Z)-phenylacetaldehyde oxime | Bacillus sp. (in: Bacteria) | isovaleronitrile + H2O | - |
? | |
| 4.8.1.4 | (E/Z)-isovaleraldoxime | 20.2% of activity with (Z)-phenylacetaldehyde oxime | Bacillus sp. (in: Bacteria) OxB-1 | isovaleronitrile + H2O | - |
? | |
| 4.8.1.4 | (E/Z)-n-butyraldoxime | 11% of activity with (Z)-phenylacetaldehyde oxime | Bacillus sp. (in: Bacteria) | n-butyronitrile + H2O | - |
? | |
| 4.8.1.4 | (E/Z)-n-butyraldoxime | 11% of activity with (Z)-phenylacetaldehyde oxime | Bacillus sp. (in: Bacteria) OxB-1 | n-butyronitrile + H2O | - |
? | |
| 4.8.1.4 | (E/Z)-n-capronaldoxime | 57.1% of activity with (Z)-phenylacetaldehyde oxime | Bacillus sp. (in: Bacteria) | n-capronitrile + H2O | - |
? | |
| 4.8.1.4 | (E/Z)-n-valeraldoxime | 39.3% of activity with (Z)-phenylacetaldehyde oxime | Bacillus sp. (in: Bacteria) | n-valeronitrile + H2O | - |
? | |
| 4.8.1.4 | (E/Z)-n-valeraldoxime | 39.3% of activity with (Z)-phenylacetaldehyde oxime | Bacillus sp. (in: Bacteria) OxB-1 | n-valeronitrile + H2O | - |
? | |
| 4.8.1.4 | (E/Z)-propionaldoxime | 8.2% of activity with (Z)-phenylacetaldehyde oxime | Bacillus sp. (in: Bacteria) | propionitrile + H2O | - |
? | |
| 4.8.1.4 | (Z)-3-phenylpropionaldoxime | 63% of activity with (Z)-phenylacetaldehyde oxime | Bacillus sp. (in: Bacteria) | phenylpropionitrile + H2O | - |
? | |
| 4.8.1.4 | (Z)-naphthoacetaldoxime | 4.5% of activity with (Z)-phenylacetaldehyde oxime | Bacillus sp. (in: Bacteria) | naphthoacetonitrile + H2O | - |
? | |
| 4.8.1.4 | (Z)-p-chlorophenylacetaldoxime | 7.3% of activity with (Z)-phenylacetaldehyde oxime | Bacillus sp. (in: Bacteria) | p-chlorophenylacetonitrile + H2O | - |
? | |
| 4.8.1.4 | (Z)-p-methoxyphenylacetaldoxime | 6.8% of activity with (Z)-phenylacetaldehyde oxime | Bacillus sp. (in: Bacteria) | p-methoxyphenylacetonitrile + H2O | - |
? | |
| 4.8.1.4 | (Z)-phenylacetaldehyde oxime | - |
Bacillus sp. (in: Bacteria) | phenylacetonitrile + H2O | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 4.8.1.4 | monomer | 1 * 40000, SDS-PAGE | Bacillus sp. (in: Bacteria) |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 4.8.1.4 | 30 | - |
- |
Bacillus sp. (in: Bacteria) |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 4.8.1.4 | 45 | - |
stable up to | Bacillus sp. (in: Bacteria) |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 4.8.1.4 | 7 | - |
- |
Bacillus sp. (in: Bacteria) |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.8.1.4 | FMN | absolute requirement | Bacillus sp. (in: Bacteria) | |
| 4.8.1.4 | heme | protoheme IX | Bacillus sp. (in: Bacteria) | |
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