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Literature summary extracted from

  • Webby, C.J.; Patchett, M.L.; Parker, E.J.
    Characterization of a recombinant type II 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase from Helicobacter pylori (2005), Biochem. J., 390, 223-230.
    View publication on PubMedView publication on EuropePMC

General Stability

EC Number General Stability Organism
2.5.1.54 -70°C, presence of 1 mM dithiothreitol, stable Helicobacter pylori
2.5.1.54 4°C, presence of 1 mM dithiothreitol, stabel for at least 24 h Helicobacter pylori

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.54 3-deoxy-D-erythro-hept-2-ulosonate 7-phosphate
-
 Helicobacter pylori
2.5.1.54 EDTA inactivation, reactivation by divalent metyl ions, in decreasing order: Co2+, Mn2+, Ca2+, Mg2+, Cu2+, Zn2+ Helicobacter pylori
2.5.1.54 additional information not inhibitory: phenylalanine, tyrosine, tryptophan, chorismate Helicobacter pylori

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.54 0.003
-
 phosphoenolpyruvate pH 7.5, 30°C Helicobacter pylori
2.5.1.54 0.006
-
 D-erythrose 4-phosphate pH 7.5, 30°C Helicobacter pylori

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.5.1.54 additional information reactivation of EDTA-treated enzyme by divalent metyl ions, in decreasing order: Co2+, Mn2+, Ca2+, Mg2+, Cu2+, Zn2+ Helicobacter pylori

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.54 51000
-
 x * 51000, SDS-PAGE Helicobacter pylori
2.5.1.54 100000
-
 gel fitlration Helicobacter pylori

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.54 Helicobacter pylori
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.54
-
 Helicobacter pylori

Reaction

EC Number Reaction Comment Organism Reaction ID
2.5.1.54 phosphoenolpyruvate + D-erythrose 4-phosphate + H2O = 3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate + phosphate ordered sequential mechanism Helicobacter pylori
a

Renatured (Commentary)

EC Number Renatured (Comment) Organism
2.5.1.54 reactivation of EDTA-treated enzyme by divalent metyl ions, in decreasing order: Co2+, Mn2+, Ca2+, Mg2+, Cu2+, Zn2+ Helicobacter pylori

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.54 additional information reaction involves interaction of si face of phosphoenolpyruvate with re face of eryxthrose 4-phosphate Helicobacter pylori ?
-
 ?
2.5.1.54 phosphoenolpyruvate + 2-deoxy-D-ribose 5-phosphate + H2O
-
 Helicobacter pylori 3,5-dideoxy-D-gluco-octulosonate 8-phosphate + 3,5-dideoxy-D-manno-octulosonate 8-phosphate + phosphate
-
 ?
2.5.1.54 phosphoenolpyruvate + D-erythrose 4-phosphate + H2O
-
 Helicobacter pylori 3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate + phosphate
-
 ?
2.5.1.54 phosphoenolpyruvate + D-ribose 5-phosphate + H2O
-
 Helicobacter pylori 3-deoxy-D-altro-octulosonate 8-phosphate + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.5.1.54 dimer x * 51000, SDS-PAGE Helicobacter pylori

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.5.1.54 3.3
-
 phosphoenolpyruvate pH 7.5, 30°C Helicobacter pylori

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.5.1.54 0.27
-
 3-deoxy-D-erythro-hept-2-ulosonate 7-phosphate pH 7.5, 30°C, cosubstrate phosphoenolpyruvate Helicobacter pylori
2.5.1.54 1.5
-
 3-deoxy-D-erythro-hept-2-ulosonate 7-phosphate pH 7.5, 30°C, cosubstrate erythrose 4-phosphate Helicobacter pylori