EC Number | Cloned (Comment) | Organism |
---|---|---|
2.4.1.1 | expression of wild-type and mutant enzymes in Escherichia coli | Corynebacterium callunae |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.4.1.1 | H334A | site-directed mutagenesis, catalytic efficiency in the phosphorolysis reaction is similar to the wild-type enzyme, 50fold reduced binding of alpha-D-glucose 1-phosphate compared to the wild-type enzyme | Corynebacterium callunae |
2.4.1.1 | H334N | site-directed mutagenesis, 50fold reduced catalytic efficiency in the phosphorolysis reaction and highly reduced selectivity for alpha-D-glucose 1-phosphate, versus alpha-D-xylose 1-phosphate, in the synthesis reaction with 10fold reduced binding of alpha-D-glucose 1-phosphate compared to the wild-type enzyme | Corynebacterium callunae |
2.4.1.1 | H334Q | site-directed mutagenesis, 150fold reduced catalytic efficiency in the phosphorolysis reaction and highly reduced selectivity for alpha-D-glucose 1-phosphate, versus alpha-D-xylose 1-phosphate, in the synthesis reaction with 10fold reduced binding of alpha-D-glucose 1-phosphate compared to the wild-type enzyme | Corynebacterium callunae |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.4.1.1 | D-gluconic acid 1,5-lactone | - |
Corynebacterium callunae |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.4.1.1 | additional information | - |
additional information | stopped-flow and steady-state kinetics, wild-type and mutant enzymes | Corynebacterium callunae | |
2.4.1.1 | 0.23 | - |
starch | pH 7.0, 30°C, recombinant wild-type enzyme, phosphorolysis | Corynebacterium callunae | |
2.4.1.1 | 0.25 | - |
starch | pH 7.0, 30°C, recombinant mutant H334A, phosphorolysis | Corynebacterium callunae | |
2.4.1.1 | 0.32 | - |
starch | pH 7.0, 30°C, recombinant mutant H334Q, phosphorolysis | Corynebacterium callunae | |
2.4.1.1 | 0.85 | - |
alpha-D-glucose 1-phosphate | pH 7.0, 30°C, recombinant wild-type enzyme, synthesis | Corynebacterium callunae | |
2.4.1.1 | 1 | - |
starch | pH 7.0, 30°C, recombinant mutant H334N, phosphorolysis | Corynebacterium callunae | |
2.4.1.1 | 1.1 | - |
alpha-D-glucose 1-phosphate | pH 7.0, 30°C, recombinant mutant H334Q, synthesis | Corynebacterium callunae | |
2.4.1.1 | 1.3 | - |
alpha-D-glucose 1-phosphate | pH 7.0, 30°C, recombinant mutant H334N, synthesis | Corynebacterium callunae | |
2.4.1.1 | 1.6 | - |
glycogen | pH 7.0, 30°C, recombinant wild-type enzyme and mutant H334A, phosphorolysis | Corynebacterium callunae | |
2.4.1.1 | 1.7 | - |
glycogen | pH 7.0, 30°C, recombinant mutant H334Q, phosphorolysis | Corynebacterium callunae | |
2.4.1.1 | 1.8 | - |
alpha-D-glucose 1-phosphate | pH 7.0, 30°C, recombinant mutant H334A, synthesis | Corynebacterium callunae | |
2.4.1.1 | 3.5 | - |
maltopentaose | pH 7.0, 30°C, recombinant mutant H334A, phosphorolysis | Corynebacterium callunae | |
2.4.1.1 | 3.8 | - |
maltopentaose | pH 7.0, 30°C, recombinant wild-type enzyme, phosphorolysis | Corynebacterium callunae | |
2.4.1.1 | 4.1 | - |
maltopentaose | pH 7.0, 30°C, recombinant mutant H334Q, phosphorolysis | Corynebacterium callunae | |
2.4.1.1 | 6.8 | - |
phosphate | pH 7.0, 30°C, recombinant wild-type enzyme, phosphorolysis | Corynebacterium callunae | |
2.4.1.1 | 12 | - |
phosphate | pH 7.0, 30°C, recombinant mutants H334A and H334Q, phosphorolysis | Corynebacterium callunae | |
2.4.1.1 | 13 | - |
phosphate | pH 7.0, 30°C, recombinant mutant H334N, phosphorolysis | Corynebacterium callunae | |
2.4.1.1 | 16 | - |
glycogen | pH 7.0, 30°C, recombinant mutant H334N, phosphorolysis | Corynebacterium callunae | |
2.4.1.1 | 103 | - |
maltopentaose | pH 7.0, 30°C, recombinant mutant H334N, phosphorolysis | Corynebacterium callunae |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.4.1.1 | Corynebacterium callunae | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.4.1.1 | recombinant wild-type and mutant enzymes from Escherichia coli | Corynebacterium callunae |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
2.4.1.1 | [(1->4)-alpha-D-glucosyl]n + phosphate = [(1->4)-alpha-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate | catalytic mechanism of alpha-retaining glucosyl transfer, His334 is involved in selectively stabilizing the oxycarbenium ion-like transition state of the reaction, ligand binding | Corynebacterium callunae |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.1.1 | (1,4-alpha-D-glucosyl)n + phosphate | polysaccharide substrates are starch and glycogen, phosphorolysis is the preferred reaction, maltotetraose is the smallest substrate accepted | Corynebacterium callunae | (1,4-alpha-D-glucosyl)n-1 + alpha-D-glucose 1-phosphate | - |
r | |
2.4.1.1 | (1,4-alpha-D-glucosyl)n-1 + alpha-D-xylose 1-phosphate | polysaccharide substrate is starch, very low activity | Corynebacterium callunae | alpha-D-xylose-(1,4-alpha-D-glucosyl)n + phosphate | - |
r | |
2.4.1.1 | glycogen + glucose 1-phosphate | - |
Corynebacterium callunae | glycogen + phosphate | - |
? | |
2.4.1.1 | maltopentaose + phosphate | - |
Corynebacterium callunae | maltotetraose + alpha-D-glucose 1-phosphate | - |
r | |
2.4.1.1 | starch + glucose 1-phosphate | - |
Corynebacterium callunae | starch + phosphate | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.4.1.1 | starch phosphorylase | - |
Corynebacterium callunae |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.4.1.1 | 30 | - |
assay at | Corynebacterium callunae |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.4.1.1 | 0.00048 | - |
alpha-D-xylose 1-phosphate | pH 7.0, 30°C, recombinant mutant H334N, synthesis | Corynebacterium callunae | |
2.4.1.1 | 0.0026 | - |
alpha-D-xylose 1-phosphate | pH 7.0, 30°C, recombinant wild-type enzyme, synthesis | Corynebacterium callunae | |
2.4.1.1 | 0.0028 | - |
alpha-D-xylose 1-phosphate | pH 7.0, 30°C, recombinant mutant H334A, synthesis | Corynebacterium callunae | |
2.4.1.1 | 0.0066 | - |
alpha-D-xylose 1-phosphate | pH 7.0, 30°C, recombinant mutant H334Q, synthesis | Corynebacterium callunae | |
2.4.1.1 | 0.52 | - |
starch | pH 7.0, 30°C, recombinant mutant H334Q, phosphorolysis | Corynebacterium callunae | |
2.4.1.1 | 0.68 | - |
alpha-D-glucose 1-phosphate | pH 7.0, 30°C, recombinant mutant H334Q, synthesis | Corynebacterium callunae | |
2.4.1.1 | 1.27 | - |
alpha-D-glucose 1-phosphate | pH 7.0, 30°C, recombinant mutant H334N, synthesis | Corynebacterium callunae | |
2.4.1.1 | 1.5 | - |
starch | pH 7.0, 30°C, recombinant mutant H334N, phosphorolysis | Corynebacterium callunae | |
2.4.1.1 | 49 | - |
alpha-D-glucose 1-phosphate | pH 7.0, 30°C, recombinant mutant H334A, synthesis | Corynebacterium callunae | |
2.4.1.1 | 52 | - |
alpha-D-glucose 1-phosphate | pH 7.0, 30°C, recombinant wild-type enzyme, synthesis | Corynebacterium callunae | |
2.4.1.1 | 74 | - |
starch | pH 7.0, 30°C, recombinant wild-type enzyme, phosphorolysis | Corynebacterium callunae | |
2.4.1.1 | 80 | - |
starch | pH 7.0, 30°C, recombinant mutant H334A, phosphorolysis | Corynebacterium callunae |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.4.1.1 | 7 | - |
assay at | Corynebacterium callunae |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
2.4.1.1 | pyridoxal 5'-phosphate | - |
Corynebacterium callunae |