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Literature summary extracted from

  • Kanugula, S.; Pegg, A.E.
    Alkylation damage repair protein O6-alkylguanine-DNA alkyltransferase from the hyperthermophiles Aquifex aeolicus and Archaeoglobus fulgidus (2003), Biochem. J., 375, 449-455.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.63 expressed at high levels in Escherichia coli fused to an N-terminal polyhistidine tag that allows a single-step isolation and purification by metal-affinity chromatography Aquifex aeolicus
2.1.1.63 expressed at high levels in Escherichia coli fused to an N-terminal polyhistidine tag that allows a single-step isolation and purification by metal-affinity chromatography Archaeoglobus fulgidus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.1.63 O6-benzylguanine
-
 Aquifex aeolicus
2.1.1.63 O6-benzylguanine
-
 Archaeoglobus fulgidus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.63 16718
-
 x * 16718, calculation from nucleotide sequence Archaeoglobus fulgidus
2.1.1.63 23000
-
-
 Aquifex aeolicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.63 DNA containing 6-O-methylguanine + [protein]-L-cysteine Aquifex aeolicus DNA-repair protein that protects cells from killing and mutagenesis by alkylating agents DNA lacking 6-O-methylguanine + [protein]-S-methyl-L-cysteine
-
 ?
2.1.1.63 DNA containing 6-O-methylguanine + [protein]-L-cysteine Archaeoglobus fulgidus DNA-repair protein that protects cells from killing and mutagenesis by alkylating agents DNA lacking 6-O-methylguanine + [protein]-S-methyl-L-cysteine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.63 Aquifex aeolicus O67467
-
-
2.1.1.63 Archaeoglobus fulgidus O27970
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.63 expressed at high levels in Escherichia coli fused to an N-terminal polyhistidine tag that allows a single-step isolation and purification by metal-affinity chromatography Aquifex aeolicus
2.1.1.63 expressed at high levels in Escherichia coli fused to an N-terminal polyhistidine tag that allows a single-step isolation and purification by metal-affinity chromatography Archaeoglobus fulgidus

Storage Stability

EC Number Storage Stability Organism
2.1.1.63 4°C, stored at, storage at -20°C or at -80°C results in protein precipitation Aquifex aeolicus
2.1.1.63 4°C, stored at, storage at -20°C or at -80°C results in protein precipitation Archaeoglobus fulgidus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.63 DNA containing 6-O-methylguanine + [protein]-L-cysteine
-
 Aquifex aeolicus DNA lacking 6-O-methylguanine + [protein]-S-methyl-L-cysteine
-
 ?
2.1.1.63 DNA containing 6-O-methylguanine + [protein]-L-cysteine
-
 Archaeoglobus fulgidus DNA lacking 6-O-methylguanine + [protein]-S-methyl-L-cysteine
-
 ?
2.1.1.63 DNA containing 6-O-methylguanine + [protein]-L-cysteine DNA-repair protein that protects cells from killing and mutagenesis by alkylating agents Aquifex aeolicus DNA lacking 6-O-methylguanine + [protein]-S-methyl-L-cysteine
-
 ?
2.1.1.63 DNA containing 6-O-methylguanine + [protein]-L-cysteine DNA-repair protein that protects cells from killing and mutagenesis by alkylating agents Archaeoglobus fulgidus DNA lacking 6-O-methylguanine + [protein]-S-methyl-L-cysteine
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.1.63 ? x * 16718, calculation from nucleotide sequence Archaeoglobus fulgidus

Synonyms

EC Number Synonyms Comment Organism
2.1.1.63 O6-alkylguanine-DNA alkyltransferase
-
 Aquifex aeolicus
2.1.1.63 O6-alkylguanine-DNA alkyltransferase
-
 Archaeoglobus fulgidus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.1.1.63 70
-
-
 Aquifex aeolicus
2.1.1.63 80
-
-
 Archaeoglobus fulgidus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.1.1.63 80 100 80°C: optimum, 100°C: substantial repair activity at Archaeoglobus fulgidus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.1.1.63 60
-
 half-life: 12 h Aquifex aeolicus
2.1.1.63 60
-
 half-life: 24 h Archaeoglobus fulgidus