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Literature summary extracted from

  • McLean, K.J.; Scrutton, N.S.; Munro, A.W.
    Kinetic, spectroscopic and thermodynamic characterization of the Mycobacterium tuberculosis adrenodoxin reductase homologue FprA (2003), Biochem. J., 372, 317-327.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.18.1.6 gene fprA, DNA and amino acid sequence determination and analysis, expression in Escherichia coli Mycobacterium tuberculosis

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.18.1.6 additional information the enzyme contains a second, kinetically distinct and inhibitory pyridine nucleotide-binding site Mycobacterium tuberculosis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.18.1.6 additional information
-
 additional information transient and steady-state kinetics of diaphorase and flavin reductase activities, determination of reduction potential Mycobacterium tuberculosis
1.18.1.6 0.0041
-
 NADPH diaphorase activity with ferricyanide, pH 7.2, 30°C Mycobacterium tuberculosis
1.18.1.6 0.051
-
 NADH diaphorase activity with ferricyanide, pH 7.2, 30°C Mycobacterium tuberculosis
1.18.1.6 0.16
-
 ferricyanide pH 7.2, 30°C Mycobacterium tuberculosis
1.18.1.6 2.5
-
 oxidized 2,6-dichloroindophenol pH 7.2, 30°C Mycobacterium tuberculosis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.18.1.6 49357
-
 x * 49357, recombinant enzyme, mass spectrometry Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.18.1.6 reduced ferredoxin + NAD(P)+ Mycobacterium tuberculosis
-
 oxidized ferredoxin + NAD(P)H
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.18.1.6 Mycobacterium tuberculosis P9WIQ3 gene fprA
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.18.1.6 recombinant enzyme from Escherichia coli to homogeneity Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.18.1.6 2 ferricyanide + NADPH diaphorase activity Mycobacterium tuberculosis 2 ferrocyanide + NADP+ + H+
-
 ?
1.18.1.6 ferricyanide + NADH
-
 Mycobacterium tuberculosis ferrocyanide + NAD+ + H+
-
 r
1.18.1.6 additional information the enzyme contains a second, kinetically distinct and inhibitory pyridine nucleotide-binding site Mycobacterium tuberculosis ?
-
 ?
1.18.1.6 NADPH + H+ + oxidized 2,6-dichlorophenolindophenol diaphorase activity Mycobacterium tuberculosis NADP+ + reduced 2,6-dichlorophenolindophenol
-
 ?
1.18.1.6 oxidized 2,6-dichlorophenolindophenol + NADPH
-
 Mycobacterium tuberculosis reduced 2,6-dichlorophenolindophenol + NADP+ + H+
-
 r
1.18.1.6 reduced ferredoxin + NAD(P)+
-
 Mycobacterium tuberculosis oxidized ferredoxin + NAD(P)H
-
 r

Subunits

EC Number Subunits Comment Organism
1.18.1.6 ? x * 49357, recombinant enzyme, mass spectrometry Mycobacterium tuberculosis

Synonyms

EC Number Synonyms Comment Organism
1.18.1.6 ferredoxin reductase
-
 Mycobacterium tuberculosis
1.18.1.6 FprA
-
 Mycobacterium tuberculosis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.18.1.6 30
-
 assay at Mycobacterium tuberculosis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.18.1.6 0.011
-
 oxidized 2,6-dichlorophenolindophenol pH 7.2, 30°C Mycobacterium tuberculosis
1.18.1.6 25.6
-
 NADH flavin reduction, pH 7.2, 30°C Mycobacterium tuberculosis
1.18.1.6 33.7
-
 ferricyanide pH 7.2, 30°C Mycobacterium tuberculosis
1.18.1.6 50.6
-
 NADPH flavin reduction, pH 7.2, 30°C Mycobacterium tuberculosis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.18.1.6 7.2
-
 assay at Mycobacterium tuberculosis

Cofactor

EC Number Cofactor Comment Organism Structure
1.18.1.6 FAD flavoenzyme, determination of reduction potential Mycobacterium tuberculosis
1.18.1.6 additional information the enzyme contains a second, kinetically distinct and inhibitory pyridine nucleotide-binding site Mycobacterium tuberculosis
1.18.1.6 NAD(P)+ preference for NADP+ Mycobacterium tuberculosis