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Literature summary extracted from

  • van Veldhoven, P.P.; van Rompuy, P.; Vanhooren, J.C.; Mannaerts, G.P.
    Purification and further characterization of peroxisomal trihydroxycoprostanoyl-CoA oxidase from rat liver (1994), Biochem. J., 304, 195-200.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.17.99.3 1,2-Butanediol activates Rattus norvegicus
1.17.99.3 1,2-propanediol activates Rattus norvegicus
1.17.99.3 1,3-Propanediol activates Rattus norvegicus
1.17.99.3 2,3-Butanediol activates Rattus norvegicus
1.17.99.3 albumin required Rattus norvegicus

General Stability

EC Number General Stability Organism
1.17.99.3 in presence of dithiothreitol, the enzyme is very stable in high concentrations of ethylene glycol,1,2-propanediol, 1,3-propanediol, 1,2-butanediol, 2,3-butanediol and 1,3-butanediol, 40-60 v/v Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.17.99.3 peroxisome
-
 Rattus norvegicus 5777
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.17.99.3 68800
-
 2 * 68800, SDS-PAGE Rattus norvegicus
1.17.99.3 120000
-
 gel filtration Rattus norvegicus
1.17.99.3 175000
-
 non-denaturing PAGE Rattus norvegicus

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
1.17.99.3 1,2-butanediol 40-60 v/v, in presence of dithiothreitol, the enzyme is very stable Rattus norvegicus
1.17.99.3 1,2-propanediol 40-60 v/v, in presence of dithiothreitol, the enzyme is very stable Rattus norvegicus
1.17.99.3 1,3-butanediol 40-60 v/v, in presence of dithiothreitol, the enzyme is very stable Rattus norvegicus
1.17.99.3 1,3-propanediol 40-60 v/v, in presence of dithiothreitol, the enzyme is very stable Rattus norvegicus
1.17.99.3 2,3-butanediol 40-60 v/v, in presence of dithiothreitol, the enzyme is very stable Rattus norvegicus
1.17.99.3 Ethylene glycol 40-60 v/v, in presence of dithiothreitol, the enzyme is very stable Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
1.17.99.3 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.17.99.3
-
 Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.17.99.3 liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.17.99.3 additional information
-
-
 Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.17.99.3 2-methylhexanoyl-CoA + H2O + acceptor
-
 Rattus norvegicus 3-hydroxy-2-methylhexanoyl-CoA + reduced acceptor
-
 ?
1.17.99.3 2-methylpalmitoyl-CoA + H2O + acceptor
-
 Rattus norvegicus 3-hydroxy-2-methylhexandecanoyl-CoA + reduced acceptor
-
 ?
1.17.99.3 pristanoyl-CoA + H2O + acceptor
-
 Rattus norvegicus 3-hydroxy-1,6,10,14-tetramethylpentadecanoyl-CoA + reduced acceptor
-
 ?
1.17.99.3 trihydroxycoprostanoyl-CoA + H2O + acceptor
-
 Rattus norvegicus 3alpha,7alpha,12alpha,24-tetrahydroxycoprostanoyl-CoA + reduced acceptor
-
 ?

Subunits

EC Number Subunits Comment Organism
1.17.99.3 dimer 2 * 68800, SDS-PAGE Rattus norvegicus

Synonyms

EC Number Synonyms Comment Organism
1.17.99.3 THCCox
-
 Rattus norvegicus
1.17.99.3 Trihydroxycoprostanoyl-CoA oxidase
-
 Rattus norvegicus

Cofactor

EC Number Cofactor Comment Organism Structure
1.17.99.3 FAD contains most likely 2 mol of loosely bound FAD per mol of enzyme Rattus norvegicus