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Literature summary extracted from

  • Valenzuela-Soto, E.M.; Marquez-Escalante, J.A.; Iturriaga, G.; Figueroa-Soto, C.G.
    Trehalose 6-phosphate synthase from Selaginella lepidophylla: purification and properties (2004), Biochem. Biophys. Res. Commun., 313, 314-319.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.1.15 D-fructose
-
Selaginella lepidophylla
2.4.1.15 D-fructose 6-phosphate
-
Selaginella lepidophylla
2.4.1.15 D-glucose
-
Selaginella lepidophylla
2.4.1.15 additional information no effect by sucrose and trehalose up tp 0.4 M Selaginella lepidophylla

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.15 additional information no effect by sucrose and trehalose up to 0.4 M, no substrate and product inhibition Selaginella lepidophylla
2.4.1.15 proline
-
Selaginella lepidophylla

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.15 Ca2+ activates at physiological concentrations Selaginella lepidophylla
2.4.1.15 K+ activates maximal at 50 mM by 176%, 160% at 400 mM Selaginella lepidophylla
2.4.1.15 Mg2+ activates at low concentration up to 5 mM Selaginella lepidophylla
2.4.1.15 additional information no effect by Na+ Selaginella lepidophylla

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.15 additional information
-
3 aggregation forms of 230, 440, and 660 kDa contain polypeptides of 50, 67, and 115 kDa polypeptides, only the 440 kDa form is catalytically active, native PAGE Selaginella lepidophylla
2.4.1.15 115000
-
4 * 115000, SDS-PAGE Selaginella lepidophylla
2.4.1.15 440000
-
native PAGE Selaginella lepidophylla

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.15 UDP-alpha-D-glucose + D-glucose 6-phosphate Selaginella lepidophylla step in trehalose biosynthesis UDP + alpha,alpha-trehalose 6-phosphate
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.15 Selaginella lepidophylla
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.4.1.15 no glycoprotein
-
Selaginella lepidophylla

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.15 native enzyme 50fold by ammonium sulfate fractionation, gel filtration, and nickel-resin affinity chromatography Selaginella lepidophylla

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.15 plant
-
Selaginella lepidophylla
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.15 7.05
-
purified enzyme Selaginella lepidophylla

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.15 additional information substrate specificity, no activity with trehalose, maltose, or fructose as acceptor substrates Selaginella lepidophylla ?
-
?
2.4.1.15 UDP-alpha-D-glucose + D-glucose 6-phosphate step in trehalose biosynthesis Selaginella lepidophylla UDP + alpha,alpha-trehalose 6-phosphate
-
?
2.4.1.15 UDP-alpha-D-glucose + D-glucose 6-phosphate the enzyme is highly specific for UDP-glucose and D-glucose 6-phosphate Selaginella lepidophylla UDP + alpha,alpha-trehalose 6-phosphate
-
?
2.4.1.15 UDP-glucose + D-fructose 6-phosphate 16% of the activity with D-glucose 6-phosphate Selaginella lepidophylla UDP + ?
-
?
2.4.1.15 UDP-glucose + D-galactose 17% of the activity with D-glucose 6-phosphate Selaginella lepidophylla UDP + ?
-
?
2.4.1.15 UDP-glucose + D-glucose 28% of the activity with D-glucose 6-phosphate Selaginella lepidophylla UDP + ?
-
?
2.4.1.15 UDP-glucose + lactose 10% of the activity with D-glucose 6-phosphate Selaginella lepidophylla UDP + ?
-
?
2.4.1.15 UDP-glucose + sucrose 19% of the activity with D-glucose 6-phosphate Selaginella lepidophylla UDP + ?
-
?

Subunits

EC Number Subunits Comment Organism
2.4.1.15 tetramer 4 * 115000, SDS-PAGE Selaginella lepidophylla

Synonyms

EC Number Synonyms Comment Organism
2.4.1.15 TPS
-
Selaginella lepidophylla
2.4.1.15 trehalose 6-phosphate synthase
-
Selaginella lepidophylla

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.15 35
-
assay at Selaginella lepidophylla

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.15 7
-
-
Selaginella lepidophylla

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.4.1.15 5 10 stable Selaginella lepidophylla

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.4.1.15 Selaginella lepidophylla 115 kDa enzyme polypeptide, isoelectric focusing
-
4.55
2.4.1.15 Selaginella lepidophylla 67 kDa enzyme polypeptide, isoelectric focusing
-
4.69
2.4.1.15 Selaginella lepidophylla 50 kDa enzyme polypeptide, isoelectric focusing
-
4.83