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Literature summary extracted from
- Purification and characterization of methionine sulfoxide reductases from mouse and Staphylococcus aureus and their substrate stereospecificity (2002), Biochem. Biophys. Res. Commun., 290, 62-65.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.8.4.11 | mrsA1, and msrA2 are transcribed as polycistronic transcript, overexpression of MsrA1, and MsrA2 as His-tagged proteins in Escherichia coliBL21(DE3) | Staphylococcus aureus |
| 1.8.4.12 | gene msrB with the codon for selenomehinonine is exchanged for methionine, overexpression in Escherichia coli | Mus musculus |
| 1.8.4.12 | pilB is transcribed as polycistronic transcript, overexpression of PilB as His-tagged protein in Escherichia coliBL21(DE3) | Staphylococcus aureus |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.8.4.11 | Staphylococcus aureus | - |
MsrA, which appears in 2 enzyme forms | - |
| 1.8.4.12 | Mus musculus | - |
MsrB is a selenoprotein | - |
| 1.8.4.12 | Staphylococcus aureus | - |
PilB | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.8.4.11 | recombinant His-tagged MsrA1, and MsrA2 from Escherichia coli by nickel affinity chromatography | Staphylococcus aureus |
| 1.8.4.12 | partial purification of native MsrB from liver, recombinant modified MsrB from Escherichia coli | Mus musculus |
| 1.8.4.12 | recombinant His-tagged PilB from Escherichia coli by nickel affinity chromatography | Staphylococcus aureus |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 1.8.4.12 | brain | - |
Mus musculus | - |
| 1.8.4.12 | cerebellum | - |
Mus musculus | - |
| 1.8.4.12 | kidney | - |
Mus musculus | - |
| 1.8.4.12 | liver | highest activity | Mus musculus | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.8.4.11 | 0.063 | - |
purified recombinant MsrA1, substrate L-methionine (S)-sulfoxide | Staphylococcus aureus |
| 1.8.4.11 | 0.24 | - |
purified recombinant MsrA2, substrate L-methionine (S)-sulfoxide | Staphylococcus aureus |
| 1.8.4.12 | additional information | - |
tissue specific activity, activity in tissues of MsrA-deficient mutant mice | Mus musculus |
| 1.8.4.12 | 0.0007 | - |
kidney, substrate L-methionine (R)-sulfoxide | Mus musculus |
| 1.8.4.12 | 0.0019 | - |
liver, substrate L-methionine (R)-sulfoxide | Mus musculus |
| 1.8.4.12 | 1.78 | - |
purified recombinant PilB, substrate L-methionine (R)-sulfoxide | Staphylococcus aureus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.8.4.11 | L-methionine (S)-sulfoxide + thioredoxin | the 2 MsrA enzymes are absolutely specific for the S-form of the substrate | Staphylococcus aureus | L-methionine + thioredoxin disulfide + H2O | - |
? |  |
| 1.8.4.12 | dabsyl-L-methionine (R)-sulfoxide + thioredoxin | the native MsrB as well as the recombinant modified MsrB show absolute specificity for the R-form of free and protein-bound methionine sulfoxide, no activity with the S-form | Mus musculus | dabsyl-L-methionine + thioredoxin disulfide + H2O | - |
? | |
| 1.8.4.12 | L-methionine (R)-sulfoxide + thioredoxin | PilB shows absolute specificity for the R-form of free and protein-bound methionine sulfoxide | Staphylococcus aureus | L-methionine + thioredoxin disulfide + H2O | - |
? | |
| 1.8.4.12 | L-methionine (R)-sulfoxide + thioredoxin | the native MsrB as well as the recombinant modified MsrB show absolute specificity for the R-form of free and protein-bound methionine sulfoxide, no activity with the S-form | Mus musculus | L-methionine + thioredoxin disulfide + H2O | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.8.4.11 | methionine sulfoxide reductase | - |
Staphylococcus aureus |
| 1.8.4.11 | MsrA | - |
Staphylococcus aureus |
| 1.8.4.12 | methionine sulfoxide reductase | - |
Staphylococcus aureus |
| 1.8.4.12 | methionine sulfoxide reductase | - |
Mus musculus |
| 1.8.4.12 | MsrB | - |
Staphylococcus aureus |
| 1.8.4.12 | MsrB | - |
Mus musculus |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.8.4.11 | 37 | - |
assay at | Staphylococcus aureus |
| 1.8.4.12 | 37 | - |
assay at | Staphylococcus aureus |
| 1.8.4.12 | 37 | - |
assay at | Mus musculus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.8.4.11 | 7.4 | - |
assay at | Staphylococcus aureus |
| 1.8.4.12 | 7.4 | - |
assay at | Staphylococcus aureus |
| 1.8.4.12 | 7.4 | - |
assay at | Mus musculus |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.8.4.11 | thioredoxin | - |
Staphylococcus aureus | |
| 1.8.4.12 | thioredoxin | - |
Staphylococcus aureus | |
| 1.8.4.12 | thioredoxin | - |
Mus musculus | |
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Release 2023.1 (January 2023)
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