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Literature summary extracted from
- A non-dechlorinating strain of Dehalospirillum multivorans: evidence for a key role of the corrinoid cofactor in the synthesis of an active tetrachloroethene dehalogenase (2002), Arch. Microbiol., 178, 443-449.
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.21.99.5 | Iron | iron-sulfur centre | Sulfurospirillum multivorans |  |
| 1.21.99.5 | sulfur | iron-sulfur centre | Sulfurospirillum multivorans | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.21.99.5 | 53000 | - |
SDS-PAGE, processed enzyme | Sulfurospirillum multivorans |
| 1.21.99.5 | 56000 | - |
SDS-PAGE, unprocessed enzyme | Sulfurospirillum multivorans |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.21.99.5 | tetrachloroethene + 2 reduced acceptor | Sulfurospirillum multivorans | via trichloroethene, enables growth on tetrachloroethene as sole energy source | cis-1,2-dichloroethene + 2 chloride + 2 acceptor + 2 H+ | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.21.99.5 | Sulfurospirillum multivorans | - |
strain N, DSM 15119, non-dechlorinating, strain K, DSM 12446, dechlorinating | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.21.99.5 | partial | Sulfurospirillum multivorans |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.21.99.5 | tetrachloroethene + 2 reduced acceptor | via trichloroethene, enables growth on tetrachloroethene as sole energy source | Sulfurospirillum multivorans | cis-1,2-dichloroethene + 2 chloride + 2 acceptor + 2 H+ | - |
? | |
| 1.21.99.5 | tetrachloroethene + reduced methyl viologen | via trichloroethene | Sulfurospirillum multivorans | cis-1,2-dichloroethene + 2 chloride + methyl viologen | - |
? | |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.21.99.5 | corrinoid | cofactor is absent in strain N and therefore the strain is unable to dechlorinate tetrachloroethene | Sulfurospirillum multivorans | |
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Release 2023.1 (January 2023)
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