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Literature summary extracted from
- Cloning and characterization of antioxidant enzyme methionine sulfoxide-S-reductase from Caenorhabditis elegans (2005), Arch. Biochem. Biophys., 434, 275-281.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.8.4.11 | gene msrA, expression in Escherichia coli strain BL21(DE3) as N-terminally His-tagged enzyme, the HIs-tag does not influence enzyme activity | Caenorhabditis elegans |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.8.4.11 | 1.18 | - |
dabsyl L-methionine-(S)-sulfoxide | pH 7.4, 37°C, recombinant enzyme | Caenorhabditis elegans |  |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.8.4.11 | 29700 | - |
x * 29700, recombinant MsrA, SDS-PAGE | Caenorhabditis elegans |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.8.4.11 | Caenorhabditis elegans | O02089 | gene msrA | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.8.4.11 | recombinant His-tagged MsrA from Escherichia coli strain BL21(DE3) by nickel affinity and ion exchange chromatography, 425fold | Caenorhabditis elegans |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.8.4.11 | 0.96 | - |
purified enzyme, substrate dabsyl L-methionine-(S)-sulfoxide with dithiothreitol | Caenorhabditis elegans |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.8.4.11 | dabsyl-L-methionine (S)-sulfoxide + dithiothreitol | synthetic substrate, MsrA is absolutely specific for the S-form | Caenorhabditis elegans | dabsyl-L-methionine + dithiothreitol disulfide + H2O | - |
? | |
| 1.8.4.11 | dabsyl-L-methionine (S)-sulfoxide + NADPH + H+ | synthetic substrate, MsrA is absolutely specific for the S-form, 7fold lower activity with NADPH compared to DTT | Caenorhabditis elegans | dabsyl-L-methionine + NADP+ + H2O | - |
? | |
| 1.8.4.11 | dabsyl-L-methionine (S)-sulfoxide + thioredoxin | synthetic substrate, MsrA is absolutely specific for the S-form | Caenorhabditis elegans | dabsyl-L-methionine + thioredoxin disulfide + H2O | - |
? | |
| 1.8.4.11 | additional information | enzyme converts free and protein-bound methionine | Caenorhabditis elegans | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.8.4.11 | ? | x * 29700, recombinant MsrA, SDS-PAGE | Caenorhabditis elegans |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.8.4.11 | methionine sulfoxide-S-reductase | - |
Caenorhabditis elegans |
| 1.8.4.11 | MsrA | - |
Caenorhabditis elegans |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.8.4.11 | 37 | - |
about | Caenorhabditis elegans |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.8.4.11 | 17 | 57 | activity rapidly decreases below 17°C and above 57°C | Caenorhabditis elegans |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.8.4.11 | 0.06 | - |
dabsyl L-methionine-(S)-sulfoxide | pH 7.4, 37°C, recombinant enzyme | Caenorhabditis elegans | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.8.4.11 | 7 | 7.5 | - |
Caenorhabditis elegans |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.8.4.11 | 6.5 | 8.5 | sharp decrease below pH 6.5 and above pH 8.5 | Caenorhabditis elegans |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.8.4.11 | dithiothreitol | preferred cofactor in vitro | Caenorhabditis elegans | |
| 1.8.4.11 | NADPH | - |
Caenorhabditis elegans | |
| 1.8.4.11 | thioredoxin | - |
Caenorhabditis elegans | |
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