EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.18.1.2 | Ferredoxin | inhibition of electron transfer at higher electron acceptor rate | Escherichia coli | |
1.18.1.2 | flavodoxinI/II | inhibition of electron transfer at higher electron acceptor rate | Escherichia coli |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.18.1.2 | additional information | - |
additional information | thermodynamics and kinetics, measurement of direct electron transfer by stopped-flow spectrophotometry | Escherichia coli | |
1.18.1.2 | 0.012 | - |
Ferredoxin | pH 8.0, 25°C | Escherichia coli | |
1.18.1.2 | 0.012 | - |
reduced ferredoxin | pH 8.0, 25°C | Escherichia coli | |
1.19.1.1 | 0.004 | - |
reduced flavodoxin II | pH 8.0, 25°C | Escherichia coli | |
1.19.1.1 | 0.0076 | - |
reduced flavodoxin I | pH 8.0, 25°C | Escherichia coli |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.18.1.2 | FMNH2 + NADP+ | Escherichia coli | - |
FMN + NADPH + H+ | - |
? | |
1.18.1.2 | additional information | Escherichia coli | the enzyme reduces flavodoxin I and flavodoxin II, reaction of EC 1.19.1.1, and ferredoxin, ferredoxin being the kinetically and thermodynamically preferred partner. Ferredoxin binds to FNR with high affinity (Kd below 0.5 microM) and is reduced under single-turnover conditions. Flavodoxin I and flavodoxin II show affinities about 4- to 7fold weaker and reduction rates that are 10- to 100fold slower than those for ferredoxin | ? | - |
? | |
1.18.1.2 | oxidized ferredoxin + NADPH | Escherichia coli | - |
reduced ferredoxin + NADP+ | - |
r | |
1.18.1.2 | reduced ferredoxin + NADP+ | Escherichia coli | - |
oxidized ferredoxin + NADPH + H+ | - |
? | |
1.19.1.1 | FMNH2 + NADP+ | Escherichia coli | - |
FMN + NADPH + H+ | - |
? | |
1.19.1.1 | additional information | Escherichia coli | the enzyme reduces flavodoxin I, flavodoxin II and ferredoxin, ferredoxin being the kinetically and thermodynamically preferred partner, i.e. reaction of EC 1.18.2.1. Flavodoxin I and flavodoxin II behave similarly with respect to FNR, with affinities about 4- to 7fold weaker and reduction rates that are 10- to 100fold slower than those for ferredoxin. Flavodoxin I and flavodoxin II can obtain electrons from reduced Fd at rates that are comparable to those obtained with reduced FNR | ? | - |
? | |
1.19.1.1 | reduced flavodoxin I + NADP+ | Escherichia coli | - |
oxidized flavodoxin I + NADPH + H+ | - |
? | |
1.19.1.1 | reduced flavodoxin II + NADP+ | Escherichia coli | - |
oxidized flavodoxin II + NADPH + H+ | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.18.1.2 | Escherichia coli | - |
- |
- |
1.18.1.2 | Escherichia coli | P28861 | enzyme catalyzes reactions of both EC 1.18.1.2 and EC 1.19.1.1 | - |
1.19.1.1 | Escherichia coli | P28861 | enzyme catalyzes reactions of both EC 1.18.1.2 and EC 1.19.1.1 | - |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
1.18.1.2 | 2 reduced ferredoxin + NADP+ + H+ = 2 oxidized ferredoxin + NADPH | reaction mechanism | Escherichia coli |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.18.1.2 | FMNH2 + NADP+ | - |
Escherichia coli | FMN + NADPH + H+ | - |
? | |
1.18.1.2 | additional information | specificity for tightly bound electron acceptors, overview | Escherichia coli | ? | - |
? | |
1.18.1.2 | additional information | the enzyme reduces flavodoxin I and flavodoxin II, reaction of EC 1.19.1.1, and ferredoxin, ferredoxin being the kinetically and thermodynamically preferred partner. Ferredoxin binds to FNR with high affinity (Kd below 0.5 microM) and is reduced under single-turnover conditions. Flavodoxin I and flavodoxin II show affinities about 4- to 7fold weaker and reduction rates that are 10- to 100fold slower than those for ferredoxin | Escherichia coli | ? | - |
? | |
1.18.1.2 | oxidized ferredoxin + NADPH | - |
Escherichia coli | reduced ferredoxin + NADP+ | - |
r | |
1.18.1.2 | oxidized ferredoxin + NADPH | ferredoxin is the preferred electron acceptor | Escherichia coli | reduced ferredoxin + NADP+ | - |
r | |
1.18.1.2 | reduced ferredoxin + NADP+ | - |
Escherichia coli | oxidized ferredoxin + NADPH + H+ | - |
r | |
1.18.1.2 | reduced ferredoxin + NADP+ | - |
Escherichia coli | oxidized ferredoxin + NADPH + H+ | - |
? | |
1.18.1.2 | reduced flavodoxin I + NADP+ | - |
Escherichia coli | oxidized flavodoxin I + NADPH + H+ | - |
r | |
1.18.1.2 | reduced flavodoxin II + NADP+ | - |
Escherichia coli | oxidized flavodoxin II + NADPH + H+ | - |
r | |
1.19.1.1 | FMNH2 + NADP+ | - |
Escherichia coli | FMN + NADPH + H+ | - |
? | |
1.19.1.1 | additional information | the enzyme reduces flavodoxin I, flavodoxin II and ferredoxin, ferredoxin being the kinetically and thermodynamically preferred partner, i.e. reaction of EC 1.18.2.1. Flavodoxin I and flavodoxin II behave similarly with respect to FNR, with affinities about 4- to 7fold weaker and reduction rates that are 10- to 100fold slower than those for ferredoxin. Flavodoxin I and flavodoxin II can obtain electrons from reduced Fd at rates that are comparable to those obtained with reduced FNR | Escherichia coli | ? | - |
? | |
1.19.1.1 | reduced flavodoxin I + NADP+ | - |
Escherichia coli | oxidized flavodoxin I + NADPH + H+ | - |
? | |
1.19.1.1 | reduced flavodoxin II + NADP+ | - |
Escherichia coli | oxidized flavodoxin II + NADPH + H+ | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.18.1.2 | ferredoxin (flavodoxin):NADP+ oxidoreductase | - |
Escherichia coli |
1.18.1.2 | FNR | - |
Escherichia coli |
1.19.1.1 | ferredoxin (flavodoxin):NADP+ oxidoreductase | - |
Escherichia coli |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.18.1.2 | 25 | - |
assay at | Escherichia coli |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.18.1.2 | 0.0039 | - |
reduced flavodoxin II | pH 8.0, 25°C | Escherichia coli | |
1.18.1.2 | 0.0042 | - |
reduced flavodoxin I | pH 8.0, 25°C | Escherichia coli | |
1.18.1.2 | 0.15 | - |
reduced ferredoxin | pH 8.0, 25°C | Escherichia coli | |
1.19.1.1 | 0.0039 | - |
reduced flavodoxin II | pH 8.0, 25°C | Escherichia coli | |
1.19.1.1 | 0.0042 | - |
reduced flavodoxin I | pH 8.0, 25°C | Escherichia coli |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.18.1.2 | 8 | - |
assay at, steady-state kinetics | Escherichia coli |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.18.1.2 | FAD | involved in electron transfer in the reaction | Escherichia coli | |
1.18.1.2 | NADP+ | - |
Escherichia coli | |
1.18.1.2 | NADPH | - |
Escherichia coli | |
1.19.1.1 | NADP+ | - |
Escherichia coli |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.18.1.2 | 0.05 | 0.1 | Ferredoxin | pH 8.0, 25°C | Escherichia coli | |
1.18.1.2 | 0.05 | 0.1 | flavodoxin I | pH 8.0, 25°C | Escherichia coli | |
1.18.1.2 | 0.05 | 0.1 | flavodoxin II | pH 8.0, 25°C | Escherichia coli |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.18.1.2 | 12.5 | - |
reduced ferredoxin | pH 8.0, 25°C | Escherichia coli | |
1.18.1.2 | 19 | - |
FMNH2 | pH 8.0, 25°C | Escherichia coli | |
1.19.1.1 | 550 | - |
reduced flavodoxin I | pH 8.0, 25°C | Escherichia coli | |
1.19.1.1 | 980 | - |
reduced flavodoxin II | pH 8.0, 25°C | Escherichia coli | |
1.19.1.1 | 19000 | - |
FMNH2 | pH 8.0, 25°C | Escherichia coli |