EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.6.1.78 | 3,4-dihydroxyphenyllactic acid | metabolite of rosmarinic acid | Anchusa officinalis | |
2.6.1.78 | additional information | enzyme shows substrate inhibition, but no feedback inhibition by L-phenylalanine and L-tyrosine | Anchusa officinalis |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.6.1.78 | 0.08 | - |
L-aspartate | pH 9.0, 40°C | Anchusa officinalis | |
2.6.1.78 | 0.08 | - |
prephenate | pH 9.0, 40°C | Anchusa officinalis |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.6.1.78 | 44000 | - |
2 * 44000 + 2 * 57000, alpha2beta2-structure, SDS-PAGE | Anchusa officinalis |
2.6.1.78 | 57000 | - |
2 * 44000 + 2 * 57000, alpha2beta2-structure, SDS-PAGE | Anchusa officinalis |
2.6.1.78 | 220000 | - |
gel filtration | Anchusa officinalis |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.6.1.78 | prephenate + L-aspartate | Anchusa officinalis | transamination | L-arogenate + oxaloacetate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.6.1.78 | Anchusa officinalis | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.6.1.78 | native enzyme 41fold to homogeneity by anion exchange chromatography, chromatofocusing using the major active fraction with an isoelectric point of 5.4, and gel filtration | Anchusa officinalis |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
2.6.1.78 | cell culture | rosmarinic acid-producing cells | Anchusa officinalis | - |
2.6.1.78 | additional information | activity during cell cycle | Anchusa officinalis | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.6.1.78 | 0.34 | - |
purified enzyme | Anchusa officinalis |
EC Number | Storage Stability | Organism |
---|---|---|
2.6.1.78 | -70°C, purified enzyme, 50 mM Tris-HCl, pH 8.2, 5 mM 2-mercaptoethanol, 150 mM sucrose, 1 mM EDTA, stable for at least 5 months | Anchusa officinalis |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.6.1.78 | 2-oxoglutarate + L-aspartate | - |
Anchusa officinalis | L-glutamate + oxaloacetate | - |
? | |
2.6.1.78 | 4-hydroxyphenylpyruvate + L-aspartate | - |
Anchusa officinalis | L-tyrosine + oxaloacetate | - |
? | |
2.6.1.78 | glyoxylate + L-aspartate | - |
Anchusa officinalis | glycine + oxaloacetate | - |
? | |
2.6.1.78 | indolepyruvate + L-aspartate | - |
Anchusa officinalis | L-tryptophan + oxaloacetate | - |
? | |
2.6.1.78 | additional information | substrate specificity, highest activity with 2-oxoglutarate and L-aspartate as substrates, low activity with indolepyruvate and glyoxylate | Anchusa officinalis | ? | - |
? | |
2.6.1.78 | phenylpyruvate + L-aspartate | - |
Anchusa officinalis | L-phenylalanine + oxaloacetate | - |
? | |
2.6.1.78 | prephenate + L-aspartate | transamination | Anchusa officinalis | L-arogenate + oxaloacetate | - |
? | |
2.6.1.78 | prephenate + L-glutamate | transamination, L-glutamate is about 3fold less effective than L-aspartate | Anchusa officinalis | L-arogenate + 2-oxoglutarate | - |
? | |
2.6.1.78 | pyruvate + L-aspartate | - |
Anchusa officinalis | L-alanine + oxaloacetate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.6.1.78 | tetramer | 2 * 44000 + 2 * 57000, alpha2beta2-structure, SDS-PAGE | Anchusa officinalis |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.6.1.78 | Pat | - |
Anchusa officinalis |
2.6.1.78 | prephenate aminotransferase | - |
Anchusa officinalis |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.6.1.78 | 60 | 70 | - |
Anchusa officinalis |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.6.1.78 | 9 | - |
- |
Anchusa officinalis |
EC Number | Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|---|
2.6.1.78 | Anchusa officinalis | minor fraction, chromatofocussing | - |
5 |
2.6.1.78 | Anchusa officinalis | major fraction, chromatofocussing | - |
5.4 |