Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • van den Broek, L.A.; van Boxtel, E.L.; Kievit, R.P.; Verhoef, R.; Beldman, G.; Voragen, A.G.
    Physico-chemical and transglucosylation properties of recombinant sucrose phosphorylase from Bifidobacterium adolescentis DSM20083 (2004), Appl. Microbiol. Biotechnol., 65, 219-227.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.7 gene sucP, cloning from genomic library, DNA and amino acid sequence determination and analysis, determination of promotor region, expression in Escherichia coli Bifidobacterium adolescentis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.7 Mg2+
-
 Bifidobacterium adolescentis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.7 56189
-
 2 * 58000, SDS-PAGE, 2 * 56189, sequence calculation Bifidobacterium adolescentis
2.4.1.7 58000
-
 2 * 58000, SDS-PAGE, 2 * 56189, sequence calculation Bifidobacterium adolescentis
2.4.1.7 129000
-
 gel filtration Bifidobacterium adolescentis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.7 sucrose + phosphate Bifidobacterium adolescentis
-
 beta-D-fructose + alpha-D-glucose 1-phosphate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.7 Bifidobacterium adolescentis Q84HQ2 DSM20083, gene sucP
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.7 recombinant enzyme from Escherichia coli Bifidobacterium adolescentis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.7 109
-
 purified recombinant enzyme, substrate sucrose Bifidobacterium adolescentis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.7 D-arabinose + alpha-D-glucose 1-phosphate
-
 Bifidobacterium adolescentis alpha-D-Glc(1-1)-beta-D-Ara + phosphate
-
 ?
2.4.1.7 D-arabitol + alpha-D-glucose 1-phosphate
-
 Bifidobacterium adolescentis ? + phosphate
-
 ?
2.4.1.7 D-fructose + alpha-D-glucose 1-phosphate low activity Bifidobacterium adolescentis ? + phosphate
-
 ?
2.4.1.7 D-fucose + alpha-D-glucose 1-phosphate low activity Bifidobacterium adolescentis ? + phosphate
-
 ?
2.4.1.7 D-galactose + alpha-D-glucose 1-phosphate low activity Bifidobacterium adolescentis ? + phosphate
-
 ?
2.4.1.7 D-glucose + alpha-D-glucose 1-phosphate low activity Bifidobacterium adolescentis ? + phosphate
-
 ?
2.4.1.7 D-sorbitol + alpha-D-glucose 1-phosphate
-
 Bifidobacterium adolescentis ? + phosphate
-
 ?
2.4.1.7 D-xylitol + alpha-D-glucose 1-phosphate high activity Bifidobacterium adolescentis ? + phosphate
-
 ?
2.4.1.7 D-xylose + alpha-D-glucose 1-phosphate low activity Bifidobacterium adolescentis ? + phosphate
-
 ?
2.4.1.7 L-arabinose + alpha-D-glucose 1-phosphate high activity Bifidobacterium adolescentis ? + phosphate
-
 ?
2.4.1.7 L-arabitol + alpha-D-glucose 1-phosphate high activity Bifidobacterium adolescentis ? + phosphate
-
 ?
2.4.1.7 L-fucose + alpha-D-glucose 1-phosphate
-
 Bifidobacterium adolescentis ? + phosphate
-
 ?
2.4.1.7 L-sorbose + alpha-D-glucose 1-phosphate
-
 Bifidobacterium adolescentis ? + phosphate
-
 ?
2.4.1.7 L-xylose + alpha-D-glucose 1-phosphate low activity Bifidobacterium adolescentis ? + phosphate
-
 ?
2.4.1.7 additional information substrate specificity, di- and trisaccharides, including sucrose, are no acceptor substrate, overview Bifidobacterium adolescentis ?
-
 ?
2.4.1.7 sucrose + phosphate
-
 Bifidobacterium adolescentis beta-D-fructose + alpha-D-glucose 1-phosphate
-
 r
2.4.1.7 sucrose + phosphate enzyme prefers the forward reaction Bifidobacterium adolescentis beta-D-fructose + alpha-D-glucose 1-phosphate
-
 r

Subunits

EC Number Subunits Comment Organism
2.4.1.7 dimer 2 * 58000, SDS-PAGE, 2 * 56189, sequence calculation Bifidobacterium adolescentis

Synonyms

EC Number Synonyms Comment Organism
2.4.1.7 More the enzyme belongs to the glycoside hydrolase family 13 Bifidobacterium adolescentis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.7 48
-
-
 Bifidobacterium adolescentis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.7 6 6.5
-
 Bifidobacterium adolescentis