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Literature summary extracted from

  • Empadinhas, N.; Albuquerque, L.; Henne, A.; Santos, H.; da Costa, M.S.
    The bacterium Thermus thermophilus, like hyperthermophilic archaea, uses a two-step pathway for the synthesis of mannosylglycerate (2003), Appl. Environ. Microbiol., 69, 3272-3279.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.217 DNA and amino acid sequence determination and analysis, genetic organization of genes involved in mannosylglycerate biosynthesis, functional overexpression in Escherichia coli strain BL21 Thermus thermophilus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.217 KCl inhibitory at 50-300 mM Thermus thermophilus
2.4.1.217 NaCl inhibitory at 50-300 mM Thermus thermophilus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.217 0.13
-
3-phospho-D-glycerate pH 8.0, 80°C, recombinant enzyme Thermus thermophilus
2.4.1.217 0.33
-
GDP-mannose pH 8.0, 80°C, recombinant enzyme Thermus thermophilus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.217 Co2+ activates, best at 2 mM Thermus thermophilus
2.4.1.217 Mg2+ activates, best at 20 mM Thermus thermophilus
2.4.1.217 Mn2+ Mn2+ is most effective in enzyme activation, best at 1 mM Thermus thermophilus
2.4.1.217 additional information strictly dependent on divalent cations, no activation by Ca2+, Sr2+, Cu2+, Ba2+, and Zn2+ Thermus thermophilus
2.4.1.217 Ni2+ activates, best at 1.5 mM Thermus thermophilus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.217 44000
-
x * 44000, recombinant enzyme, SDS-PAGE Thermus thermophilus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.217 GDP-mannose + 3-phospho-D-glycerate Thermus thermophilus
-
GDP + 2-(alpha-D-mannosyl)-3-phosphoglycerate
-
?
2.4.1.217 GDP-mannose + 3-phospho-D-glycerate Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039
-
GDP + 2-(alpha-D-mannosyl)-3-phosphoglycerate
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.217 Thermus thermophilus Q84B24 AY193871, GenBank sequence encoding mannosyl-3-phosphoglycerate synthase and mannosyl-3-phosphoglycerate phosphatase
-
2.4.1.217 Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039 Q84B24 AY193871, GenBank sequence encoding mannosyl-3-phosphoglycerate synthase and mannosyl-3-phosphoglycerate phosphatase
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.217 recombinant enzyme from Escherichia coli strain BL21 Thermus thermophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.217 GDP-mannose + 3-phospho-D-glycerate
-
Thermus thermophilus GDP + 2-(alpha-D-mannosyl)-3-phosphoglycerate
-
?
2.4.1.217 GDP-mannose + 3-phospho-D-glycerate
-
Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039 GDP + 2-(alpha-D-mannosyl)-3-phosphoglycerate
-
?

Subunits

EC Number Subunits Comment Organism
2.4.1.217 ? x * 44000, recombinant enzyme, SDS-PAGE Thermus thermophilus

Synonyms

EC Number Synonyms Comment Organism
2.4.1.217 MPGS
-
Thermus thermophilus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.217 80 90 recombinant enzyme Thermus thermophilus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.4.1.217 50 100 temperature-profile, 10% of maximal activity at 25°C, 55% of maximal activity at 103°C Thermus thermophilus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.1.217 80
-
half-life 189 min Thermus thermophilus
2.4.1.217 90
-
half-life 22 min Thermus thermophilus
2.4.1.217 100
-
half-life 10 min Thermus thermophilus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.217 7
-
about, recombinant enzyme Thermus thermophilus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.4.1.217 5 9 pH-profile, 30-40% of maximal activity at pH 5.0 and pH 9.0 Thermus thermophilus