BRENDA - Enzyme Database

Mutagenesis analysis of a conserved region involved in acetyl coenzyme A binding in the aminoglycoside 6'-N-acetyltransferase type Ib encoded by plasmid pJHCMW1

Pourreza, A.; Witherspoon, M.; Fox, J.; Newmark, J.; Bui, D.; Tolmasky, M.E.; Antimicrob. Agents Chemother. 49, 2979-2982 (2005)

Data extracted from this reference:

Engineering
EC Number
Protein Variants
Commentary
Organism
2.3.1.82
D117A
complete loss of the resistance phenotype against kanamycin and amikacin
Escherichia coli
2.3.1.82
D120A
complete loss of the resistance phenotype against kanamycin and amikacin
Escherichia coli
Organism
EC Number
Organism
UniProt
Commentary
Textmining
2.3.1.82
Escherichia coli
-
encoded by plasmid pJHCMW1
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
2.3.1.82
acetyl-CoA + amikacin
-
657563
Escherichia coli
?
-
-
-
?
2.3.1.82
acetyl-CoA + kanamycin
-
657563
Escherichia coli
CoA + N6'-acetylkanamycin
-
-
-
?
Synonyms
EC Number
Synonyms
Commentary
Organism
2.3.1.82
aminoglycoside 6'-N-acetyltransferase type Ib
-
Escherichia coli
Engineering (protein specific)
EC Number
Protein Variants
Commentary
Organism
2.3.1.82
D117A
complete loss of the resistance phenotype against kanamycin and amikacin
Escherichia coli
2.3.1.82
D120A
complete loss of the resistance phenotype against kanamycin and amikacin
Escherichia coli
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
2.3.1.82
acetyl-CoA + amikacin
-
657563
Escherichia coli
?
-
-
-
?
2.3.1.82
acetyl-CoA + kanamycin
-
657563
Escherichia coli
CoA + N6'-acetylkanamycin
-
-
-
?