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Literature summary extracted from

  • Masuda, M.; Tsunoda, M.; Imai, K.
    High-performance liquid chromatography-fluorescent assay of catechol-O-methyltransferase activity in rat brain (2003), Anal. Bioanal. Chem., 376, 1069-1073.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.6 0.0055
-
 norepinephrine corebral cortex, membrane-bound form Rattus norvegicus
2.1.1.6 0.00587
-
 norepinephrine cerebellum, membrane-bound form Rattus norvegicus
2.1.1.6 0.00697
-
 S-adenosyl-L-methionine soluble enzyme Rattus norvegicus
2.1.1.6 0.00837
-
 norepinephrine brain stem, membrane-bound form Rattus norvegicus
2.1.1.6 0.00912
-
 norepinephrine hippocampus, membrane-bound form Rattus norvegicus
2.1.1.6 0.0105
-
 norepinephrine hypophysis, membrane-bound form Rattus norvegicus
2.1.1.6 0.0113
-
 S-adenosyl-L-methionine membrane-bound enzyme Rattus norvegicus
2.1.1.6 0.0114
-
 norepinephrine hypothalamus, membrane-bound form Rattus norvegicus
2.1.1.6 0.304
-
 norepinephrine corebral cortex, soluble enzyme Rattus norvegicus
2.1.1.6 0.305
-
 norepinephrine hippocampus, soluble enzyme Rattus norvegicus
2.1.1.6 0.313
-
 norepinephrine cerebellum, soluble enzyme Rattus norvegicus
2.1.1.6 0.39
-
 norepinephrine brain stem, soluble enzyme Rattus norvegicus
2.1.1.6 0.443
-
 norepinephrine hypothalamus, soluble enzyme Rattus norvegicus
2.1.1.6 0.465
-
 norepinephrine hypophysis, soluble enzyme Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.1.1.6 membrane bound to.In all brain areas membrane-bound enzyme activity is lower than soluble enzyme activity Rattus norvegicus 16020
-
2.1.1.6 soluble in all brain areas membrane-bound enzyme activity is lower than soluble enzyme activity Rattus norvegicus
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.1.1.6 Mg2+ Km: 0.22 mM for soluble enzyme, 0.17 mM for membrane-bound enzyme Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.6 Rattus norvegicus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.1.6 brain highest activity in hippocampus. In all brain areas membrane-bound enzyme activity is lower than soluble enzyme activity Rattus norvegicus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.6 S-adenosyl-L-methionine + 3,4-dihydroxybenzoic acid
-
 Rattus norvegicus S-adenosyl-L-homocysteine + ?
-
 ?
2.1.1.6 S-adenosyl-L-methionine + norepinephrine
-
 Rattus norvegicus S-adenosyl-L-homocysteine + ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.1.1.6 COMT
-
 Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.6 7
-
 membrane-bound enzyme Rattus norvegicus
2.1.1.6 7.8
-
 soluble enzyme Rattus norvegicus