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Literature summary extracted from

  • Guo, S.; Bhattacharjee, J.K.
    Posttranslational activation, site-directed mutation and phylogenetic analyses of the lysine biosynthesis enzymes alpha-aminoadipate reductase Lys1p (AAR) and the phosphopantetheinyl transferase Lys7p (PPTase) from Schizosaccharomyces pombe (2004), Yeast, 21, 1279-1288.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.2.1.31 ATP
-
 Schizosaccharomyces pombe

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.2.1.31 expression in Escherichia coli as an active enzyme. Exhibits significant alpha-aminoadipate reductase activity without the addition of CoA and Lys7p PPTase from Schizosaccharomyces pombe Schizosaccharomyces pombe
1.2.1.95 expression in Escherichia coli Schizosaccharomyces pombe

Protein Variants

EC Number Protein Variants Comment Organism
1.2.1.31 C125I mutant enzyme shows no alpha-aminoadipate reductase activity Schizosaccharomyces pombe
1.2.1.31 D126E mutant enzyme shows no alpha-aminoadipate reductase activity Schizosaccharomyces pombe
1.2.1.31 D94E mutant enzyme shows no alpha-aminoadipate reductase activity Schizosaccharomyces pombe
1.2.1.31 E173D mutant enzyme shows no alpha-aminoadipate reductase activity Schizosaccharomyces pombe
1.2.1.31 F93W mutant enzyme shows no alpha-aminoadipate reductase activity Schizosaccharomyces pombe
1.2.1.31 F95W mutant enzyme shows no alpha-aminoadipate reductase activity Schizosaccharomyces pombe
1.2.1.31 G124A mutant enzyme shows no alpha-aminoadipate reductase activity Schizosaccharomyces pombe
1.2.1.31 G79A mutant enzyme shows no alpha-aminoadipate reductase activity Schizosaccharomyces pombe
1.2.1.31 G910A mutant enzyme shows no alpha-aminoadipate reductase activity Schizosaccharomyces pombe
1.2.1.31 K172R mutant enzyme shows no alpha-aminoadipate reductase activity Schizosaccharomyces pombe
1.2.1.31 K177R mutant enzyme shows no alpha-aminoadipate reductase activity Schizosaccharomyces pombe
1.2.1.31 N96D mutant enzyme shows no alpha-aminoadipate reductase activity Schizosaccharomyces pombe
1.2.1.31 P81A mutant enzyme shows no alpha-aminoadipate reductase activity Schizosaccharomyces pombe
1.2.1.31 R80K mutant enzyme shows no alpha-aminoadipate reductase activity Schizosaccharomyces pombe
1.2.1.31 S913A mutant enzyme shows no alpha-aminoadipate reductase activity Schizosaccharomyces pombe
1.2.1.31 S913T mutant enzyme shows no alpha-aminoadipate reductase activity Schizosaccharomyces pombe
1.2.1.95 G910A mutation in the activation domain (IGGHSI), no activity Schizosaccharomyces pombe
1.2.1.95 additional information enzyme Lys1 is active when expressed in Escherichia coli and exhibits significant alpha-aminoadipate reductase activity without the addition of CoA or Schizosaccharomyces pombe phosphopantetheinyl transferase Schizosaccharomyces pombe
1.2.1.95 S913A mutation in the activation domain (IGGHSI), no activity Schizosaccharomyces pombe
1.2.1.95 S913T mutation in the activation domain (IGGHSI), no activity Schizosaccharomyces pombe

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.1.31 L-2-aminoadipate + NADPH + H+ Schizosaccharomyces pombe
-
 L-2-aminoadipate 6-semialdehyde + NADP+ + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.31 Schizosaccharomyces pombe
-
-
-
1.2.1.95 Schizosaccharomyces pombe P40976
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
1.2.1.95 side-chain modification enzyme Lys1 is active when expressed in Escherichia coli and exhibits significant alpha-aminoadipate reductase activity without the addition of CoA or Schizosaccharomyces pombe phosphopantetheinyl transferase Schizosaccharomyces pombe

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.31
-
 Schizosaccharomyces pombe

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.31 L-2-aminoadipate + NADPH + H+
-
 Schizosaccharomyces pombe L-2-aminoadipate 6-semialdehyde + NADP+ + H2O
-
 ?
1.2.1.31 L-2-aminoadipate + NADPH + H+ in presence of ATP Schizosaccharomyces pombe L-2-aminoadipate 6-semialdehyde + NADP+ + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.2.1.31 AAR
-
 Schizosaccharomyces pombe
1.2.1.31 alpha-aminoadipate reductase Lys1p
-
 Schizosaccharomyces pombe
1.2.1.31 Lys1p
-
 Schizosaccharomyces pombe

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.31 NADPH
-
 Schizosaccharomyces pombe