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Literature summary extracted from

  • Padyana, A.K.; Burley, S.K.
    Crystal structure of shikimate 5-dehydrogenase (SDH) bound to NADP: insights into function and evolution (2003), Structure, 11, 1005-1013.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.25 gene aroE, expression of GST-tagged SeMet-labeled enzyme in Escherichia coli strain BL21 Methanocaldococcus jannaschii

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.1.1.25 in complex with NADP+, compact alpha/beta sandwich with two domains for binding substrate and cofactor, resp. Methanocaldococcus jannaschii
1.1.1.25 purified recombinant AroE bound to cofactor NADP+, hanging drop vapour diffusion method at 25°C, 10 mg/ml protein in 20 mM HEPES, pH 7.1, and 0.1 M KCl, is mixed with reservoir solution containing 20 mM HEPES, pH 7.1, 20% PEG 3350, and 0.2 M ammonium fluoride, cryoprotection with 15% glycerol, X-ray diffraction structure determination and analysis at 2.35 A resolution Methanocaldococcus jannaschii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.25 31690
-
 recombinant AroE, mass spectrometry Methanocaldococcus jannaschii
1.1.1.25 31691
-
 x * 31691, recombinant AroE, mass spectrometry Methanocaldococcus jannaschii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.25 shikimate + NADP+ Methanocaldococcus jannaschii
-
 3-dehydroshikimate + NADPH + H+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.25 Methanocaldococcus jannaschii Q58484
-
-
1.1.1.25 Methanocaldococcus jannaschii Q58484 gene aroE
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.25 recombinant GST-tagged SeMet-labeled enzyme from Escherichia coli strain BL21 by glutathione affinity and ion exchange chromatography, proteolytic removal of the GST-tag Methanocaldococcus jannaschii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.25 shikimate + NADP+
-
 Methanocaldococcus jannaschii 3-dehydroshikimate + NADPH + H+
-
 r

Subunits

EC Number Subunits Comment Organism
1.1.1.25 ? x * 31691, recombinant AroE, mass spectrometry Methanocaldococcus jannaschii
1.1.1.25 More the enzyme shows an alpha/beta sandwich with two distinct domains, responsible for binding substrate and the NADP cofactor, respectively, a phylogenetically conserved deep cleft on the protein surface corresponds to the enzyme active site, the structure reveals a topologically unique domain fold within the N-terminal segment of the polypeptide chain, which binds substrate and supports dimerization, homology modeling, overview Methanocaldococcus jannaschii

Synonyms

EC Number Synonyms Comment Organism
1.1.1.25 SDH
-
 Methanocaldococcus jannaschii
1.1.1.25 shikimate 5-dehydrogenase
-
 Methanocaldococcus jannaschii

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.25 NADP+
-
 Methanocaldococcus jannaschii
1.1.1.25 NADPH
-
 Methanocaldococcus jannaschii