Literature summary extracted from

Sakasegawa, S.I.; Hagemeier, C.H.; Thauer, R.K.; Essen, L.O.; Shima, S.
Structural and functional analysis of the gpsA gene product of Archaeoglobus fulgidus: A glycerol-3-phosphate dehydrogenase with an unusual NADP+ preference (2004), Protein Sci., 13, 3161-3171.

Crystallization (Commentary)

EC Number	Crystallization (Comment)	Organism
1.1.1.177	-	Archaeoglobus fulgidus

Inhibitors

EC Number	Inhibitors	Comment	Organism	Structure
1.1.1.177	D-glyceraldehyde 3-phosphate	-	Archaeoglobus fulgidus
1.1.1.177	L-alpha-glycerol 3-phosphate	-	Archaeoglobus fulgidus
1.1.1.177	NADP+	-	Archaeoglobus fulgidus
1.1.1.177	NADPH	-	Archaeoglobus fulgidus

KM Value [mM]

EC Number	KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
1.1.1.177	0.04	-	NADPH	pH 6.6, 70°C	Archaeoglobus fulgidus
1.1.1.177	0.1	-	L-alpha-glycerol 3-phosphate	pH 6.6, 70°C	Archaeoglobus fulgidus
1.1.1.177	0.8	-	NADP+	pH 6.6, 70°C	Archaeoglobus fulgidus
1.1.1.177	1	-	D-glyceraldehyde 3-phosphate	pH 6.6, 70°C	Archaeoglobus fulgidus

Localization

EC Number	Localization	Comment	Organism	GeneOntology No.	Textmining

Organism

EC Number	Organism	UniProt	Comment	Textmining
1.1.1.177	Archaeoglobus fulgidus	-	recombinant enzyme	-

Specific Activity [micromol/min/mg]

EC Number	Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
1.1.1.177	4	-	pH 6.6, 70°C, substrate L-alpha-glycerol 3-phosphate	Archaeoglobus fulgidus
1.1.1.177	44	-	pH 6.6, 70°C, substrate D-glyceraldehyde 3-phosphate	Archaeoglobus fulgidus

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
1.1.1.177	D-glyceraldehyde 3-phosphate + NADPH	-	Archaeoglobus fulgidus	L-alpha-glycerol 3-phosphate + NADP+	-	r
1.1.1.177	L-alpha-glycerol 3-phosphate + NADP+	-	Archaeoglobus fulgidus	D-glyceraldehyde 3-phosphate + NADPH	-	r
1.1.1.177	additional information	no enzymic activity with dihydroxyacetone, glycerol, glycerol-2-phosphate, D-glyceraldehyde-3-phosphate, DL-glyceraldehyde, D-erythrose-4-phosphate, D-fructose-4-phosphate	Archaeoglobus fulgidus	?	-	?

Subunits

EC Number	Subunits	Comment	Organism
1.1.1.177	dimer	crystallization data	Archaeoglobus fulgidus

Temperature Optimum [°C]

EC Number	Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
1.1.1.177	70	-	-	Archaeoglobus fulgidus

Temperature Stability [°C]

EC Number	Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
1.1.1.177	85	-	30 min, in presence of 200 mM potassium phosphate or 100 mM NaCl or 50 mM ammonium sulfate, completely stable	Archaeoglobus fulgidus

pH Optimum

EC Number	pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
1.1.1.177	5	-	or below, reduction reaction	Archaeoglobus fulgidus
1.1.1.177	9.5	-	or above, oxidation reaction	Archaeoglobus fulgidus

Cofactor

EC Number	Cofactor	Comment	Organism	Structure
1.1.1.177	NADP+	-	Archaeoglobus fulgidus
1.1.1.177	NADPH	-	Archaeoglobus fulgidus

Ki Value [mM]

EC Number	Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
1.1.1.177	additional information	-	additional information	KI-value for NADPH is below 0.005 mM	Archaeoglobus fulgidus
1.1.1.177	0.005	-	NADP+	pH 6.6, 70°C	Archaeoglobus fulgidus
1.1.1.177	0.1	-	D-glyceraldehyde 3-phosphate	pH 6.6, 70°C	Archaeoglobus fulgidus
1.1.1.177	17	-	L-alpha-glycerol 3-phosphate	pH 6.6, 70°C	Archaeoglobus fulgidus

pI Value

EC Number	Organism	Comment	pI Value Maximum	pI Value
1.1.1.177	Archaeoglobus fulgidus	deduced from gene sequence	-	5

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Release 2023.1 (January 2023)