EC Number | Cloned (Comment) | Organism |
---|---|---|
3.6.1.23 | expressed in Escherichia coli | Leishmania major |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.6.1.23 | alpha-beta-imido-dUTP | competitive, strong inhibition | Leishmania major | |
3.6.1.23 | dUDP | competitive | Leishmania major | |
3.6.1.23 | dUMP | competitive | Leishmania major | |
3.6.1.23 | additional information | diphosphate has no effect on activity | Leishmania major |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.6.1.23 | 0.0021 | - |
dUTP | pH 8, 25°C | Leishmania major | |
3.6.1.23 | 0.0627 | - |
dUDP | pH 8, 25°C | Leishmania major |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.6.1.23 | Co2+ | can partially replace Mg2+, causes increase in Km | Leishmania major | |
3.6.1.23 | Mg2+ | required, no activity in the absence of Mg2+ | Leishmania major | |
3.6.1.23 | Mn2+ | can partially replace Mg2+, causes increase in Km | Leishmania major |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.6.1.23 | 30300 | - |
2 * 30300, calculated from amino acid sequence | Leishmania major |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.6.1.23 | dUTP + H2O | Leishmania major | removes dUTP from the nucleotide triphosphate pool and therefore prevents the incorporation of uracil into the DNA, essential for replication and cell survival | dUMP + diphosphate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.6.1.23 | Leishmania major | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.6.1.23 | - |
Leishmania major |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.6.1.23 | dATP + H2O | low activity | Leishmania major | dAMP + diphosphate | - |
? | |
3.6.1.23 | dCTP + H2O | low activity | Leishmania major | dCMP + diphosphate | - |
? | |
3.6.1.23 | dGTP + H2O | low activity | Leishmania major | dGMP + diphosphate | - |
? | |
3.6.1.23 | dTTP + H2O | low activity | Leishmania major | dTMP + diphosphate | - |
? | |
3.6.1.23 | dUDP + H2O | - |
Leishmania major | dUMP + phosphate | - |
? | |
3.6.1.23 | dUTP + H2O | - |
Leishmania major | dUMP + diphosphate | - |
? | |
3.6.1.23 | dUTP + H2O | removes dUTP from the nucleotide triphosphate pool and therefore prevents the incorporation of uracil into the DNA, essential for replication and cell survival | Leishmania major | dUMP + diphosphate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.6.1.23 | dimer | 2 * 30300, calculated from amino acid sequence | Leishmania major |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.6.1.23 | dUTPase-dUDPase | hydrolyzes dUTP and dUDP | Leishmania major |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.6.1.23 | 50 | - |
- |
Leishmania major |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.6.1.23 | 4 | 60 | very low activity above 60°C | Leishmania major |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.6.1.23 | 4 | 55 | stable against denaturation | Leishmania major |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.6.1.23 | 5.4 | - |
dATP | pH 8, 25°C | Leishmania major | |
3.6.1.23 | 6.4 | - |
dGTP | pH 8, 25°C | Leishmania major | |
3.6.1.23 | 8.6 | - |
dTTP | pH 8, 25°C | Leishmania major | |
3.6.1.23 | 13 | - |
dCTP | pH 8, 25°C | Leishmania major | |
3.6.1.23 | 49 | - |
dUTP | pH 8, 25°C | Leishmania major | |
3.6.1.23 | 65 | - |
dUDP | pH 8, 25°C | Leishmania major |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.6.1.23 | 8 | 10 | - |
Leishmania major |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
3.6.1.23 | 7 | 10 | strong increase of activity between pH 7 and 8 | Leishmania major |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.6.1.23 | 0.0009 | - |
alpha-beta-imido-dUTP | pH 8, 25°C | Leishmania major |