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Literature summary extracted from

  • Seidle, H.F.; Marten, I.; Shoseyov, O.; Huber, R.E.
    Physical and kinetic properties of the family 3 beta-glucosidase from Aspergillus niger which is important for cellulose breakdown (2004), Protein J., 23, 11-23.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.21 cellobiose
-
 Aspergillus niger
3.2.1.21 cellotriose
-
 Aspergillus niger
3.2.1.21 D-allose
-
 Aspergillus niger
3.2.1.21 D-galactose
-
 Aspergillus niger
3.2.1.21 D-gluconolactone
-
 Aspergillus niger
3.2.1.21 D-glucose
-
 Aspergillus niger
3.2.1.21 D-mannose
-
 Aspergillus niger
3.2.1.21 D-xylose
-
 Aspergillus niger
3.2.1.21 gentiobiose
-
 Aspergillus niger
3.2.1.21 maltose
-
 Aspergillus niger

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.21 0.3
-
 cellotetraose 25°C, pH 4.5 Aspergillus niger
3.2.1.21 0.4
-
 cellopentaose 25°C, pH 4.5 Aspergillus niger
3.2.1.21 0.5
-
 cellotriose 25°C, pH 4.5 Aspergillus niger
3.2.1.21 1
-
 p-nitrophenyl-beta-D-glucopyranoside 25°C, pH 4.5 Aspergillus niger
3.2.1.21 1.3
-
 gentiobiose 25°C, pH 4.5 Aspergillus niger
3.2.1.21 1.5
-
 laminaribose 25°C, pH 4.5 Aspergillus niger
3.2.1.21 1.5
-
 4-O-gentiobiosyl-glucose 25°C, pH 4.5 Aspergillus niger
3.2.1.21 2.7
-
 cellobiose 25°C, pH 4.5 Aspergillus niger
3.2.1.21 3.2
-
 o-nitrophenyl-beta-D-glucopyranoside 25°C, pH 4.5 Aspergillus niger
3.2.1.21 3.9
-
 sophorose 25°C, pH 4.5 Aspergillus niger
3.2.1.21 4.6
-
 isotrehalose 25°C, pH 4.5 Aspergillus niger

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.21 91400
-
 2 * 91400, deduced from nucleotide sequence Aspergillus niger
3.2.1.21 120000
-
 2 * 120000, SDS-PAGE Aspergillus niger
3.2.1.21 210000
-
 native-PAGE Aspergillus niger
3.2.1.21 223000
-
 gel filtration Aspergillus niger

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.21 Aspergillus niger
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.21 glycoprotein N-glycosylation Aspergillus niger

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.21 4-O-gentiobiosyl-glucose + H2O
-
 Aspergillus niger ?
-
 ?
3.2.1.21 cellobiose + H2O Bgs also has transglucosidic activity Aspergillus niger 2 beta-D-glucose
-
 ?
3.2.1.21 cellopentaose + H2O
-
 Aspergillus niger cellotetraose + beta-D-glucose
-
 ?
3.2.1.21 cellotetraose + H2O
-
 Aspergillus niger cellotriose + D-glucose
-
 ?
3.2.1.21 cellotriose + H2O
-
 Aspergillus niger cellobiose + beta-D-glucose
-
 ?
3.2.1.21 gentiobiose + H2O
-
 Aspergillus niger 2 beta-D-glucose
-
 ?
3.2.1.21 isotrehalose + H2O
-
 Aspergillus niger beta-D-glucose
-
 ?
3.2.1.21 laminaribiose + H2O
-
 Aspergillus niger 2 beta-D-glucose
-
 ?
3.2.1.21 o-nitrophenyl-beta-D-glucopyranose + H2O
-
 Aspergillus niger o-nitrophenol + beta-D-glucose
-
 ?
3.2.1.21 p-nitrophenyl-beta-D-glucopyranose + H2O
-
 Aspergillus niger p-nitrophenol + beta-D-glucose
-
 ?
3.2.1.21 sophorose + H2O
-
 Aspergillus niger 2 beta-D-glucose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.21 dimer 2 * 120000, SDS-PAGE Aspergillus niger
3.2.1.21 dimer 2 * 91400, deduced from nucleotide sequence Aspergillus niger

Synonyms

EC Number Synonyms Comment Organism
3.2.1.21 BGs
-
 Aspergillus niger

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.21 4
-
-
 Aspergillus niger

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.21 3 6 approx. 70% of maximal activity at pH 3.0, approx. 5% of maximal activity at pH 6.0 Aspergillus niger

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.2.1.21 0.4
-
 cellotriose 25°C, pH 4.5 Aspergillus niger
3.2.1.21 0.4
-
 D-gluconolactone 25°C, pH 4.5 Aspergillus niger
3.2.1.21 3
-
 D-glucose 25°C, pH 4.5 Aspergillus niger
3.2.1.21 3.3
-
 cellobiose 25°C, pH 4.5 Aspergillus niger
3.2.1.21 4.7
-
 gentiobiose 25°C, pH 4.5 Aspergillus niger
3.2.1.21 260
-
 D-mannose 25°C, pH 4.5 Aspergillus niger
3.2.1.21 260
-
 D-allose 25°C, pH 4.5 Aspergillus niger
3.2.1.21 320
-
 maltose 25°C, pH 4.5 Aspergillus niger
3.2.1.21 340
-
 D-xylose 25°C, pH 4.5 Aspergillus niger
3.2.1.21 670
-
 D-galactose 25°C, pH 4.5 Aspergillus niger