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Literature summary extracted from

  • Zhang, L.; Zhao, C.; Zhu, D.; Ohta, Y.; Wang, Y.
    Purification and characterization of inulinase from Aspergillus niger AF10 expressed in Pichia pastoris (2004), Protein Expr. Purif., 35, 272-275.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.80 DDT at 55ºC, pH 4.5, 5 mM, 121% of the relative activity without metal ions Aspergillus niger

Application

EC Number Application Comment Organism
3.2.1.80 nutrition production of ultra high fructose syrup Aspergillus niger

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.80 expression in Pichia pastoris Aspergillus niger

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.80 Al3+ at 55ºC, pH 4.5, 5 mM, 89% inhibition Aspergillus niger
3.2.1.80 Cu2+ at 55ºC, pH 4.5, 5 mM, 20% inhibition Aspergillus niger
3.2.1.80 D-glucose at 55ºC, pH 4.5, concentration higher than 2% Aspergillus niger
3.2.1.80 Fe3+ at 55ºC, pH 4.5, 5 mM, 45% inhibition Aspergillus niger
3.2.1.80 Hg2+ at 55ºC, pH 4.5, 5 mM, 91% inhibition Aspergillus niger
3.2.1.80 Mg2+ at 55ºC, pH 4.5, 5 mM, 41% inhibition Aspergillus niger
3.2.1.80 additional information no inhibited by EDTA and 2-mercaptoethanol Aspergillus niger
3.2.1.80 Zn2+ at 55ºC, pH 4.5, 5 mM, 8% inhibition Aspergillus niger

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.80 additional information
-
 additional information Michaelis-Menten kinetics Aspergillus niger
3.2.1.80 2.57
-
 inulin at 55ºC, pH 4.5 Aspergillus niger

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.80 Ca2+ at 55ºC, pH 4.5, 5 mM, 138% of the relative activity without metal ions Aspergillus niger
3.2.1.80 Fe2+ at 55ºC, pH 4.5, 5 mM, 123% of the relative activity without metal ions Aspergillus niger
3.2.1.80 Mn2+ at 55ºC, pH 4.5, 5 mM, 112% of the relative activity without metal ions Aspergillus niger

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.80 83000
-
 SDS-PAGE Aspergillus niger

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.80 inulin + 5 H2O Aspergillus niger
-
 5 beta-D-fructofuranose + alpha-D-glucopyranose
-
 ?
3.2.1.80 inulin + 5 H2O Aspergillus niger AF10
-
 5 beta-D-fructofuranose + alpha-D-glucopyranose
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.80 Aspergillus niger O74641 AF10
-
3.2.1.80 Aspergillus niger AF10 O74641 AF10
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.80 no modification
-
 Aspergillus niger

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.80 recombinant enzyme, by ammonium sulfate precipitation and anion exchange column, 4.8fold Aspergillus niger

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.80 156
-
 at 55ºC, pH 4.5 Aspergillus niger

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.80 inulin + 5 H2O
-
 Aspergillus niger 5 beta-D-fructofuranose + alpha-D-glucopyranose
-
 ?
3.2.1.80 inulin + 5 H2O
-
 Aspergillus niger AF10 5 beta-D-fructofuranose + alpha-D-glucopyranose
-
 ?
3.2.1.80 sucrose + H2O
-
 Aspergillus niger beta-D-fructofuranose + alpha-D-glucopyranose
-
 ?
3.2.1.80 sucrose + H2O
-
 Aspergillus niger AF10 beta-D-fructofuranose + alpha-D-glucopyranose
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.80 exo-beta-D-fructosidase
-
 Aspergillus niger
3.2.1.80 exoinulinase
-
 Aspergillus niger

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.80 55
-
 pH 5.5 Aspergillus niger

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.80 4.5
-
 at 55ºC Aspergillus niger