Literature summary extracted from
Barney, B.M.; Schaab, M.R.; LoBrutto, R.; Francisco, W.A.
Evidence for a new metal in a known active site: purification and characterization of an iron-containing quercetin 2,3-dioxygenase from Bacillus subtilis (2004), Protein Expr. Purif., 35, 131-141.
Activating Compound
EC Number |
Activating Compound |
Comment |
Organism |
Structure |
---|
1.13.11.24 |
Tiron |
0.8 mM, 25% activation |
Bacillus subtilis |
|
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
1.13.11.24 |
- |
Bacillus subtilis |
General Stability
EC Number |
General Stability |
Organism |
---|
1.13.11.24 |
storage in buffer, loss of 25% of activity during 48 h, high concentrations of ammonium chloride stabilize, ascorbic acid and cysteine do not |
Bacillus subtilis |
Inhibitors
EC Number |
Inhibitors |
Comment |
Organism |
Structure |
---|
1.13.11.24 |
1,10-phenanthroline |
0.21 mM, 46% residual activity |
Bacillus subtilis |
|
1.13.11.24 |
2,2'-dipyridyl |
0.18 mM, 57% residual activity |
Bacillus subtilis |
|
1.13.11.24 |
diethyldithiocarbamate |
89 mM, 62% residual activity |
Bacillus subtilis |
|
1.13.11.24 |
EDTA |
94 mM, 94% residual activity |
Bacillus subtilis |
|
1.13.11.24 |
kojic acid |
5 mM, 50% residual activity |
Bacillus subtilis |
|
1.13.11.24 |
O-ethylxanthate |
6 mM, 51% residual activity |
Bacillus subtilis |
|
KM Value [mM]
EC Number |
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Comment |
Organism |
Structure |
---|
1.13.11.24 |
0.0008 |
- |
quercetin |
pH 7.5, 25°C |
Bacillus subtilis |
|
Molecular Weight [Da]
EC Number |
Molecular Weight [Da] |
Molecular Weight Maximum [Da] |
Comment |
Organism |
---|
1.13.11.24 |
37640 |
- |
x * 37640, MALDI-TOF, SDS-PAGE |
Bacillus subtilis |
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
1.13.11.24 |
Bacillus subtilis |
- |
expression in Escherichia coli |
- |
Specific Activity [micromol/min/mg]
EC Number |
Specific Activity Minimum [µmol/min/mg] |
Specific Activity Maximum [µmol/min/mg] |
Comment |
Organism |
---|
1.13.11.24 |
1.5 |
- |
25°C, pH 7.5 |
Bacillus subtilis |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
1.13.11.24 |
quercetin + O2 |
- |
Bacillus subtilis |
2-protocatechoylphloroglucinolcarboxylate + CO |
- |
? |
|
Subunits
EC Number |
Subunits |
Comment |
Organism |
---|
1.13.11.24 |
? |
x * 37640, MALDI-TOF, SDS-PAGE |
Bacillus subtilis |
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
1.13.11.24 |
YxaG |
- |
Bacillus subtilis |
pH Optimum
EC Number |
pH Optimum Minimum |
pH Optimum Maximum |
Comment |
Organism |
---|
1.13.11.24 |
7.5 |
- |
- |
Bacillus subtilis |
pH Stability
EC Number |
pH Stability |
pH Stability Maximum |
Comment |
Organism |
---|
1.13.11.24 |
5 |
- |
precipitates below |
Bacillus subtilis |
pI Value
EC Number |
Organism |
Comment |
pI Value Maximum |
pI Value |
---|
1.13.11.24 |
Bacillus subtilis |
calculated |
- |
4.9 |