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Literature summary extracted from

  • Schulte, D.; Hengstenberg, W.
    Engineering the active center of the 6-phospho-beta-galactosidase from Lactococcus lactis (2000), Protein Eng., 13, 515-518.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.85 expressed in Escherichia coli K12 TG1 Lactococcus lactis

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.85 K435V/Y437F significantly reduced substrate binding and turnover Lactococcus lactis
3.2.1.85 S428D/K435V/Y437F no activity Lactococcus lactis
3.2.1.85 W429A improved specificity for glucosides Lactococcus lactis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.85 0.32
-
 o-nitrophenyl-beta-D-galactoside wild type enzyme, pH 7.5, room temperature Lactococcus lactis
3.2.1.85 0.39
-
 p-nitrophenyl-beta-D-galactopyranoside 6-phosphate wild type enzyme, pH 7.5, room temperature Lactococcus lactis
3.2.1.85 0.54
-
 o-nitrophenyl-beta-D-galactopyranoside 6-phosphate wild type enzyme, pH 7.5, room temperature Lactococcus lactis
3.2.1.85 0.57
-
 o-nitrophenyl-beta-D-galactoside K435V/Y437F mutant enzyme, pH 7.5, room temperature Lactococcus lactis
3.2.1.85 1.56
-
 o-nitrophenyl-beta-D-glucopyranoside 6-phosphate wild type enzyme, pH 7.5, room temperature Lactococcus lactis
3.2.1.85 2.86
-
 o-nitrophenyl-beta-D-galactopyranoside 6-phosphate W429A mutant enzyme, pH 7.5, room temperature Lactococcus lactis
3.2.1.85 2.99
-
 p-nitrophenyl-beta-D-glucopyranoside 6-phosphate wild type enzyme, pH 7.5, room temperature Lactococcus lactis
3.2.1.85 3.1
-
 p-nitrophenyl-beta-D-galactopyranoside 6-phosphate W429A mutant enzyme, pH 7.5, room temperature Lactococcus lactis
3.2.1.85 4.89
-
 o-nitrophenyl-beta-D-galactopyranoside 6-phosphate K435V/Y437F mutant enzyme, pH 7.5, room temperature Lactococcus lactis
3.2.1.85 9.83
-
 o-nitrophenyl-beta-D-glucopyranoside 6-phosphate W429A mutant enzyme, pH 7.5, room temperature Lactococcus lactis
3.2.1.85 21.53
-
 p-nitrophenyl-beta-D-glucopyranoside 6-phosphate W429A mutant enzyme, pH 7.5, room temperature Lactococcus lactis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.85 lactose-6-phosphate + H2O Lactococcus lactis involved in lactose assimilation D-glucose + beta-D-galactose-6-phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.85 Lactococcus lactis P11546
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.85
-
 Lactococcus lactis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.85 lactose-6-phosphate + H2O
-
 Lactococcus lactis D-glucose + beta-D-galactose-6-phosphate
-
 ?
3.2.1.85 lactose-6-phosphate + H2O involved in lactose assimilation Lactococcus lactis D-glucose + beta-D-galactose-6-phosphate
-
 ?
3.2.1.85 o-nitrophenyl-beta-D-galactopyranoside 6-phosphate + H2O
-
 Lactococcus lactis o-nitrophenol + beta-D-galactose-6-phosphate
-
 ?
3.2.1.85 o-nitrophenyl-beta-D-galactoside + H2O
-
 Lactococcus lactis o-nitrophenol + beta-D-galactose
-
 ?
3.2.1.85 o-nitrophenyl-beta-D-glucopyranoside 6-phosphate + H2O
-
 Lactococcus lactis o-nitrophenol + beta-D-glucose-6-phosphate
-
 ?
3.2.1.85 p-nitrophenyl-beta-D-galactopyranoside 6-phosphate + H2O less effective than ortho-form Lactococcus lactis p-nitrophenol + beta-D-galactose-6-phosphate
-
 ?
3.2.1.85 p-nitrophenyl-beta-D-glucopyranoside 6-phosphate + H2O less effective than ortho-form Lactococcus lactis p-nitrophenol + beta-D-glucose-6-phosphate
-
 ?