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Literature summary extracted from
- Excision of misincorporated ribonucleotides in DNA by RNase H (type 2) and FEN-1 in cell-free extracts (2002), Proc. Natl. Acad. Sci. USA, 99, 16654-16659.
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 3.1.26.4 | additional information | construction of several deletion mutants | Saccharomyces cerevisiae |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.26.4 | KCl | - |
Escherichia coli |  |
| 3.1.26.4 | KCl | - |
Homo sapiens | |
| 3.1.26.4 | KCl | - |
Saccharomyces cerevisiae | |
| 3.1.26.4 | KCl | - |
Pyrococcus furiosus | |
| 3.1.26.4 | Mg2+ | - |
Escherichia coli | |
| 3.1.26.4 | Mg2+ | - |
Homo sapiens | |
| 3.1.26.4 | Mg2+ | - |
Saccharomyces cerevisiae | |
| 3.1.26.4 | Mg2+ | - |
Pyrococcus furiosus | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.26.4 | Escherichia coli | - |
gene rnhb, type 2 enzyme, wild-type DY330 and knock-out strain | - |
| 3.1.26.4 | Homo sapiens | - |
RNase H1 is a type 2 enzyme | - |
| 3.1.26.4 | Pyrococcus furiosus | - |
type 2 enzyme | - |
| 3.1.26.4 | Saccharomyces cerevisiae | - |
type 2 enzyme | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.1.26.4 | partially | Homo sapiens |
| 3.1.26.4 | partially | Saccharomyces cerevisiae |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.1.26.4 | HeLa cell | - |
Homo sapiens | - |
| 3.1.26.4 | JURKAT cell | - |
Homo sapiens | - |
| 3.1.26.4 | additional information | 90% purified recombinant enzyme | Pyrococcus furiosus | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.26.4 | dsDNA oligonucleotide with a single ribose + H2O | enzyme excises misincorporated ribonucleotides in DNA | Pyrococcus furiosus | dsDNA oligonucleotide with 1 nt gap + 5'-monophosphate ribonucleotide | - |
? | |
| 3.1.26.4 | dsDNA oligonucleotide with a single ribose + H2O | enzyme excises misincorporated ribonucleotides in DNA, enzyme places the first 5' nick, while the second 3' cut is made by FEN-1 protein | Homo sapiens | dsDNA oligonucleotide with 1 nt gap + 5'-monophosphate ribonucleotide | - |
? | |
| 3.1.26.4 | dsDNA oligonucleotide with a single ribose + H2O | preferred substrate, enzyme excises misincorporated ribonucleotides in DNA | Escherichia coli | dsDNA oligonucleotide with 1 nt gap + 5'-monophosphate ribonucleotide | - |
? | |
| 3.1.26.4 | dsDNA oligonucleotide with a stretch of ribonucleotides + H2O | enzyme excises misincorporated ribonucleotides in DNA | Saccharomyces cerevisiae | dsDNA oligonucleotide with 1 nt gap + 5'-monophosphate ribonucleotide | - |
? | |
| 3.1.26.4 | dsDNA oligonucleotides with a single ribose + H2O | preferred substrate, enzyme excises misincorporated ribonucleotides in DNA, enzyme places the first 5' nick, while the second 3' cut is made by Rad27p | Saccharomyces cerevisiae | dsDNA oligonucleotides with 1 nt gap + 5'-monophosphate ribonucleotide | - |
? | |
| 3.1.26.4 | additional information | substrate specificity | Escherichia coli | ? | - |
? | |
| 3.1.26.4 | additional information | substrate specificity | Homo sapiens | ? | - |
? | |
| 3.1.26.4 | additional information | substrate specificity, enzyme plays a role in the repair of misincorporated ribonucleotides rather than or in addition to processing RNA*DNA hybrid molecules | Saccharomyces cerevisiae | ? | - |
? | |
| 3.1.26.4 | RNA*DNA hybrid + H2O | very low activity | Escherichia coli | ? | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.1.26.4 | RNase H | - |
Escherichia coli |
| 3.1.26.4 | RNase H | - |
Homo sapiens |
| 3.1.26.4 | RNase H | - |
Saccharomyces cerevisiae |
| 3.1.26.4 | RNase H type 2 | - |
Saccharomyces cerevisiae |
| 3.1.26.4 | RNase H(35) | - |
Saccharomyces cerevisiae |
| 3.1.26.4 | RNase H1 | - |
Homo sapiens |
| 3.1.26.4 | RNase HII | - |
Escherichia coli |
| 3.1.26.4 | RNase HII | - |
Pyrococcus furiosus |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.26.4 | 37 | - |
assay at | Escherichia coli |
| 3.1.26.4 | 37 | - |
assay at | Homo sapiens |
| 3.1.26.4 | 37 | - |
assay at | Saccharomyces cerevisiae |
| 3.1.26.4 | 37 | - |
assay at | Pyrococcus furiosus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.26.4 | 7.5 | - |
assay at | Escherichia coli |
| 3.1.26.4 | 7.5 | - |
assay at | Homo sapiens |
| 3.1.26.4 | 7.5 | - |
assay at | Saccharomyces cerevisiae |
| 3.1.26.4 | 7.5 | - |
assay at | Pyrococcus furiosus |
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