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Literature summary extracted from

  • Vothknecht, U.C.; Kannangara, C.G.; von Wettstein, D.
    Expression of catalytically active barley glutamyl tRNAGlu reductase in Escherichia coli as a fusion protein with glutathione S-transferase (1996), Proc. Natl. Acad. Sci. USA, 93, 9287-9291.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.2.1.70 expression in Escherichia coli as a fusion protein with glutathione S-transferase Hordeum vulgare

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.2.1.70 hemin 50% inhibition at 0.0015 mM Hordeum vulgare

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.1.70 60000
-
 4 * 60000, SDS-PAGE Hordeum vulgare
1.2.1.70 250000
-
 gel filtration Hordeum vulgare

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.1.70 L-glutamyl-tRNAGlu + NADPH + H+ Hordeum vulgare the enzyme catalyzes the initial step of tetrapyrrole biosynthesis L-glutamate 1-semialdehyde + NADP+ + tRNAGlu
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.70 Hordeum vulgare Q42843
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.70 fusion protein with glutathione S-transferase Hordeum vulgare

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2.1.70 0.12
-
 fusion protein with glutathione S-transferase Hordeum vulgare

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.70 L-glutamyl-tRNAGlu + NADPH + H+ the enzyme catalyzes the initial step of tetrapyrrole biosynthesis Hordeum vulgare L-glutamate 1-semialdehyde + NADP+ + tRNAGlu
-
 ?
1.2.1.70 L-glutamyl-tRNAGlu + NADPH + H+ the fusion protein with glutathione S-transferase uses tRNAGlu from Hordeum vulgare chloroplast preferentially to Escherichia coli tRNAGlu Hordeum vulgare L-glutamate 1-semialdehyde + NADP+ + tRNAGlu
-
 ?

Subunits

EC Number Subunits Comment Organism
1.2.1.70 tetramer 4 * 60000, SDS-PAGE Hordeum vulgare

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.70 heme the fusion protein with glutathione S-transferase contains heme, which can be reduced by NADPH and oxidized by air Hordeum vulgare
1.2.1.70 NADPH
-
 Hordeum vulgare