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Literature summary extracted from

  • Sobajima, H.; Takeda, M.; Sugimori, M.; Kobashi, N.; Kiribuchi, K.; Cho, E.M.; Akimoto, C.; Yamaguchi, T.; Minami, E.; Shibuya, N.; Schaller, F.; Weiler, E.W.; Yoshihara, T.; Nishida, H.; Nojiri, H.; Omori, T.; Nishiyama, M.; Yamane, H.
    Cloning and characterization of a jasmonic acid-responsive gene encoding 12-oxophytodienoic acid reductase in suspension-cultured rice cells (2003), Planta, 216, 692-698.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.1.42 construction of a cDNA library, phylogenetic analysis, DNA and amino acid sequence determination and analysis, expression as His-tagged enzyme in Escherichia coli strain BL21(DE3) Oryza sativa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.3.1.42 41000
-
 x * 41000, recombinant His-tagged enzyme, SDS-PAGE Oryza sativa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.1.42 (15Z)-12-oxophyto-10,15-dienoate + NADPH Oryza sativa leads to jasmonate biosynthesis 8-[(1R,2R)-3-oxo-2-((Z)-pent-2-enyl)cyclopentyl]octanoate + NADP+
-
 ?
1.3.1.42 additional information Oryza sativa physiological role of the enzyme ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.42 Oryza sativa Q84QK0 cv. BL-1, enzyme OsOPR1
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.1.42 recombinant His-tagged enzyme from Escherichia coli by nickel affinity chromatography Oryza sativa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.3.1.42 cell suspension culture
-
 Oryza sativa
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.42 (15Z)-12-oxophyto-10,15-dienoate + NADPH leads to jasmonate biosynthesis Oryza sativa 8-[(1R,2R)-3-oxo-2-((Z)-pent-2-enyl)cyclopentyl]octanoate + NADP+
-
 ?
1.3.1.42 (15Z)-12-oxophyto-10,15-dienoate + NADPH preference of (-)-cis isomer over the (+)-cis-isomer Oryza sativa 8-[(1R,2R)-3-oxo-2-((Z)-pent-2-enyl)cyclopentyl]octanoate + NADP+
-
 ?
1.3.1.42 additional information physiological role of the enzyme Oryza sativa ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.3.1.42 ? x * 41000, recombinant His-tagged enzyme, SDS-PAGE Oryza sativa

Synonyms

EC Number Synonyms Comment Organism
1.3.1.42 OPR
-
 Oryza sativa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.3.1.42 25
-
 assay at Oryza sativa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.3.1.42 7.5
-
 assay at Oryza sativa

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.1.42 NADPH
-
 Oryza sativa