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Literature summary extracted from

  • Sharma, P.; Dubey, R.S.
    Ascorbate peroxidase from rice seedlings: properties of enzyme isoforms, effects of stresses and protective roles of osmolytes (2004), Plant Sci., 167, 541-550.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.11.1.11 3,3'-dithiobis(6-nitrobenzoic acid) 5 mM, 80% residual activity, APX 1, 24% residual activity, APX 2 Oryza sativa
1.11.1.11 Al3+ inhibition in the range of 0.01-0.5 mM Oryza sativa
1.11.1.11 KCN 1 mM, 69% residual activity, APX 1, 20% residual activity, APX 2 Oryza sativa
1.11.1.11 additional information not inhibitory: alpha,alphaย’-dipyridyl, EDTA Oryza sativa
1.11.1.11 Na2HAsO4 inhibition in the range of 0.01-0.5 mM Oryza sativa
1.11.1.11 Ni2+ below 0.01 mM, activation, inhibition above Oryza sativa
1.11.1.11 Sodium azide 1 mM, 72% residual activity, APX 1, 55% residual activity, APX 2 Oryza sativa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.11.1.11 0.033
-
 H2O2 pH 6.5, APX 1 Oryza sativa
1.11.1.11 0.076
-
 H2O2 pH 7.0, APX 2 Oryza sativa
1.11.1.11 0.2
-
 ascorbate pH 7.0, APX 2 Oryza sativa
1.11.1.11 0.4
-
 L-ascorbate pH 6.5, APX 1 Oryza sativa

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.11.1.11 Ni2+ below 0.01 mM, activation, inhibition above Oryza sativa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.11.1.11 28500
-
 x * 28500, APX 1, x * 34000, APX 2, SDS-PAGE Oryza sativa
1.11.1.11 34000
-
 x * 28500, APX 1, x * 34000, APX 2, SDS-PAGE Oryza sativa

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
1.11.1.11 Ethylene glycol PEG 6000, 40% in assay medium, 50% inhibition of aPX 1, 65% inhibition of APX 2 with increase in enzyme Km values, incorporation of 1 M proline, glycine betaine or sucrose in presence of PEG in the assay medium restores Oryza sativa

Organism

EC Number Organism UniProt Comment Textmining
1.11.1.11 Oryza sativa
-
 isoforms APX 1, APX 2
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.11.1.11 both APX 1 and APX 2 Oryza sativa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.11.1.11 leaf
-
 Oryza sativa
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.11.1.11 glutathione + H2O2 30% of the activity with ascorbate, APX 1, 13% of the activity with ascorbate, APX 2 Oryza sativa ? + H2O
-
 ?
1.11.1.11 guaiacol + H2O2 45% of the activity with ascorbate, APX 1, 15% of the activity with ascorbate, APX 2 Oryza sativa ? + H2O
-
 ?
1.11.1.11 L-ascorbate + H2O2
-
 Oryza sativa dehydroascorbate + H2O
-
 ?
1.11.1.11 NADH + H+ + H2O2 10% of the activity with ascorbate, APX 1, 1% of the activity with ascorbate, APX 2 Oryza sativa NAD+ + H2O
-
 ?
1.11.1.11 NADPH + H+ + H2O2 27% of the activity with ascorbate, APX 1, 21% of the activity with ascorbate, APX 2 Oryza sativa NADP+ + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.11.1.11 ? x * 28500, APX 1, x * 34000, APX 2, SDS-PAGE Oryza sativa

Synonyms

EC Number Synonyms Comment Organism
1.11.1.11 APX 1
-
 Oryza sativa
1.11.1.11 APX 2
-
 Oryza sativa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.11.1.11 6.5
-
 APX 1 Oryza sativa
1.11.1.11 7
-
 APX 2 Oryza sativa