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Literature summary extracted from

  • Iwamoto, K.; Kawanobe, H.; Ikawa, T.; Shiraiwa, Y.
    Characterization of salt-regulated mannitol-1-phosphate dehydrogenase in the red alga Caloglossa continua (2003), Plant Physiol., 133, 893-900.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.17 fructose-6-phosphate substrate inhibition above 0.5 mM Caloglossa continua
1.1.1.17 N-ethylmaleimide 1 mM, 80% inhibition of reduction and oxidation reaction Caloglossa continua
1.1.1.17 p-hydroxymercuribenzoate 0.5 mM, complete inhibition of reduction and oxidation reaction, 2-mercaptoethanol protects Caloglossa continua

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.17 3.5
-
 NADH pH 7.0, 25°C Caloglossa continua
1.1.1.17 5.4
-
 NADH pH 7.0, 25°C, presence of 200 mM NaCl Caloglossa continua
1.1.1.17 32
-
 NAD+ pH 7.0, 25°C Caloglossa continua
1.1.1.17 48.9
-
 D-mannitol-1-phosphate pH 7.0, 25°C Caloglossa continua
1.1.1.17 56.9
-
 NADH pH 7.0, 25°C, presence of 200 mM NaCl Caloglossa continua
1.1.1.17 138
-
 D-mannitol-1-phosphate pH 7.0, 25°C, presence of 200 mM NaCl Caloglossa continua
1.1.1.17 188.8
-
 D-fructose-6-phosphate pH 7.0, 25°C Caloglossa continua
1.1.1.17 1458
-
 D-fructose-6-phosphate pH 7.0, 25°C, presence of 200 mM NaCl Caloglossa continua

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.17 53000
-
 gel filtration Caloglossa continua

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.17 Caloglossa continua
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.17
-
 Caloglossa continua

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.17 228.6
-
 reduction, pH 7.0, 25°C, presence of 100 mM NaCl Caloglossa continua

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.17 D-fructose-6-phosphate + NADH
-
 Caloglossa continua D-mannitol-1-phosphate + NAD+
-
 r
1.1.1.17 D-mannitol-1-phosphate + NAD+
-
 Caloglossa continua D-fructose-6-phosphate + NADH
-
 r
1.1.1.17 additional information high specificity for substrates fructose-6-phosphate/NADH and mannitol-1-phosphate/NAD+, no substrate: fructose-1-phosphate, fructose-1,6-bisphosphate, glucose-1-phosphate, NADPH, NADP+ Caloglossa continua ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.17 monomer 1 * 53000, SDS-PAGE Caloglossa continua

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.17 6
-
 reduction, optimum may be below pH 6.0 Caloglossa continua
1.1.1.17 7.2
-
 reduction, in presence of 200 mM NaCl Caloglossa continua
1.1.1.17 9
-
 oxidation, assay at Caloglossa continua

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.1.1.17 Caloglossa continua isoelectric focusing
-
 5.5