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Literature summary extracted from

  • Fukuchi-Mizutani, M.; Okuhara, H.; Fukui, Y.; Nakao, M.; Katsumoto, Y.; Yonekura-Sakakibara, K.; Kusumi, T.; Hase, T.; Tanaka, Y.
    Biochemical and molecular characterization of a novel UDP-glucose:anthocyanin 3'-O-glucosyltransferase, a key enzyme for blue anthocyanin biosynthesis, from gentian (2003), Plant Physiol., 132, 1652-1663.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
2.4.1.238 agriculture 3'GT cDNA is a useful molecular tool for modification of flower color using genetic engineering, production of blue anthocyanins Gentiana triflora

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.238 expression in transgenic Petunia hybrida and in Escherichia coli JM105, sequencing Gentiana triflora

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.238 Al3+ 80% inhibition Gentiana triflora
2.4.1.238 Co2+ 69% inhibition Gentiana triflora
2.4.1.238 additional information not inhibited by Mg2+, Ca2+, Mn2+, EDTA or EGTA Gentiana triflora
2.4.1.238 UDP competitive inhibition Gentiana triflora
2.4.1.238 Zn2+ 26% inhibition Gentiana triflora

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.238 0.12
-
 delphinidin 3,5-diglucoside pH 8.5, 30°C, recombinant 3'GT Gentiana triflora

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.238 soluble recombinant 3'GT expressed in Escherichia coli JM105 Gentiana triflora
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.238 54037
-
 x * 55000, SDS-PAGE, x * 54037, sequence calculation Gentiana triflora
2.4.1.238 55000
-
 x * 55000, SDS-PAGE, x * 54037, sequence calculation Gentiana triflora

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.238 additional information Gentiana triflora key enzyme for blue anthocyanin biosynthesis, biosynthesis of gentiodelphin ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.238 Gentiana triflora Q8H0F2 var. japonica
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.238 native 3'GT from petals, recombinant 3'GT expressed in Escherichia coli JM105 Gentiana triflora

Reaction

EC Number Reaction Comment Organism Reaction ID
2.4.1.238 UDP-alpha-D-glucose + delphinidin 3,5-di-O-beta-D-glucoside = UDP + delphinidin 3,3',5-tri-O-beta-D-glucoside this enzyme specifically glucosylates the 3'-hydroxy group of delphinidin-3-O-glucosyl-5-O-(6-O-caffeoylglucosyl)-3'-O-(6-O-caffeoylglucoside)-type anthocyanins containing glucose groups at the 3 and 5 positions, acts on delphinidin 3,5-di-O-glucoside in gentian, Gentiana triflora Gentiana triflora
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.238 additional information 3'GT mRNA is barely detectable in leaves Gentiana triflora
-
2.4.1.238 petal blue, from flowers at a pre-anthesis stage, 3'GT mRNA is abundant in petals at the first three developmental stages, but dramatically decreased in fully opened flowers Gentiana triflora
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.238 additional information key enzyme for blue anthocyanin biosynthesis, biosynthesis of gentiodelphin Gentiana triflora ?
-
 ?
2.4.1.238 additional information no sugar donors: UDP-galactose, UDP-GlcUA, UDP-N-acetylgalactosamine, not: pelargonidin 3,5-diglucoside, cyanidin 3,5-diglucoside, dihydroflavonols, dihydrokaempferol, dihydroquercetin, dihydromyricetin, flavone, tricetin Gentiana triflora ?
-
 ?
2.4.1.238 UDP-alpha-D-glucose + delphinidin 3-O-glucosyl-5-O-(6-O-caffeoylglucoside) in vitro, 50.9% of the activity with delphinidin 3,5-diglucoside Gentiana triflora UDP + delphinidin 3-O,3'-O-di-beta-D-glucosyl-5-O-(6-O-caffeoyl-beta-D-glucoside)
-
 ?
2.4.1.238 UDP-glucose + an anthocyanidin strict substrate specificity, 3'GT specifically glucosylates the 3'-hydroxy group of delphinidin-type anthocyanins containing glucose groups at the 3 and 5 positions and specifically uses UDP-glucose as the sugar donor, 3’GT strictly requires three hydroxy groups on the B-ring for its substrates Gentiana triflora UDP + an anthocyanidin 3'-O-beta-D-glucoside
-
 ?
2.4.1.238 UDP-glucose + delphinidin poor substrate, 3.3% of the activity with delphinidin 3,5-diglucoside Gentiana triflora UDP + delphinidin 3'-glucoside
-
 ?
2.4.1.238 UDP-glucose + delphinidin 3,5-di-O-beta-D-glucoside 3'GT glucosylates the 3'-hydroxy group of delphinidin 3,5-diglucoside, 2fold higher activity than with delphinidin 3-O-glucosyl-5-O-(6-O-caffeoylglucoside) as substrate Gentiana triflora UDP + delphinidin 3,5,7-tri-O-beta-D-glucoside
-
 ?
2.4.1.238 UDP-glucose + delphinidin 3-glucoside poor substrate, 2.9% of the activity with delphinidin 3,5-diglucoside Gentiana triflora UDP + delphinidin 3,3'-diglucoside
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.1.238 ? x * 55000, SDS-PAGE, x * 54037, sequence calculation Gentiana triflora

Synonyms

EC Number Synonyms Comment Organism
2.4.1.238 3'GT
-
 Gentiana triflora
2.4.1.238 UDP-glucose:anthocyanin 3'-O-glucosyltransferase
-
 Gentiana triflora

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.238 30
-
 assay at Gentiana triflora

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.238 8.5
-
 assay at Gentiana triflora

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.4.1.238 0.76
-
 UDP pH 8.5, 30°C, recombinant 3'GT Gentiana triflora