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Literature summary extracted from

  • Menting, J.G.T.; Scopes, R.K.; Stevenson, T.W.
    Characterization of flavonoid 3',5'-hydroxylase in microsomal membrane fraction of Petunia hybrida flowers (1994), Plant Physiol., 106, 633-642.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.14.14.81 2-mercaptoethanol maximal activation at 20 mM Petunia x hybrida

General Stability

EC Number General Stability Organism
1.14.14.81 activity declines when microsomal suspensions are subjected to freezing and thawing Petunia x hybrida

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.14.81 1-aminobenzotriazole
-
 Petunia x hybrida
1.14.14.81 CO
-
 Petunia x hybrida
1.14.14.81 cytochrome c 0.05 mM, complete inhibition Petunia x hybrida
1.14.14.81 decyl-beta-D-glucopyranoside dose-dependent inhibition Petunia x hybrida
1.14.14.81 diethyldithiocarbamate
-
 Petunia x hybrida
1.14.14.81 Digitonin dose-dependent inhibition Petunia x hybrida
1.14.14.81 dodecyl maltoside dose-dependent inhibition Petunia x hybrida
1.14.14.81 Emulgen 911 dose-dependent inhibition Petunia x hybrida
1.14.14.81 Emulgen 913 dose-dependent inhibition Petunia x hybrida
1.14.14.81 heptyl-beta-D-glucopyranoside dose-dependent inhibition Petunia x hybrida
1.14.14.81 hexyl-beta-D-glucopyranoside dose-dependent inhibition Petunia x hybrida
1.14.14.81 Lubrol Px dose-dependent inhibition Petunia x hybrida
1.14.14.81 Mega-10 dose-dependent inhibition Petunia x hybrida
1.14.14.81 Mega-8 dose-dependent inhibition Petunia x hybrida
1.14.14.81 Mega-9 dose-dependent inhibition Petunia x hybrida
1.14.14.81 additional information a polyclonal antibody that inhibits higher plant NADPH-cytochrome p450 reductase inhibits the flavonoid 3',5'-hydroxylase. No effect by diethylpyrocarbonate or phenylmethylsulfonyl fluoride Petunia x hybrida
1.14.14.81 NEM
-
 Petunia x hybrida
1.14.14.81 Nonidet P40 dose-dependent inhibition Petunia x hybrida
1.14.14.81 nonyl-beta-D-glucopyranoside dose-dependent inhibition Petunia x hybrida
1.14.14.81 octyl-beta-D-glucopyranoside dose-dependent inhibition Petunia x hybrida
1.14.14.81 sodium cholate dose-dependent inhibition Petunia x hybrida
1.14.14.81 sodium deoxycholate dose-dependent inhibition Petunia x hybrida
1.14.14.81 Tetcyclacis
-
 Petunia x hybrida
1.14.14.81 Triton X-100 dose-dependent inhibition Petunia x hybrida
1.14.14.81 Triton X-114 dose-dependent inhibition Petunia x hybrida
1.14.14.81 Tween 20 dose-dependent inhibition Petunia x hybrida
1.14.14.81 Tween 80 dose-dependent inhibition Petunia x hybrida
1.14.14.81 Zwittergent dose-dependent inhibition Petunia x hybrida
1.14.14.81 Zwittergent 3-08 dose-dependent inhibition Petunia x hybrida
1.14.14.81 Zwittergent 3-10 dose-dependent inhibition Petunia x hybrida
1.14.14.81 Zwittergent 3-12 dose-dependent inhibition Petunia x hybrida
1.14.14.81 Zwittergent 3-14 dose-dependent inhibition Petunia x hybrida
1.14.14.81 Zwittergent 3-16 dose-dependent inhibition Petunia x hybrida

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.14.81 microsome microsomal membrane Petunia x hybrida
-
-

Organism

EC Number Organism UniProt Comment Textmining
1.14.14.81 Petunia x hybrida
-
 cv. Old Glory Blue
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.14.81 flower bud activity varies with the development of flowers, peaking immediately prior to and during anthesis, but is absent in mature flowers Petunia x hybrida
-

Storage Stability

EC Number Storage Stability Organism
1.14.14.81 4°C, stable overnight in presence of 20% glycerol or sucrose at pH 7.5 Petunia x hybrida
1.14.14.81 75°C, storage in presence of 20% sucrose for periods of greater than 1 month results in significant loss of enzyme activity Petunia x hybrida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.14.81 5,7,3',4'-tetrahydroxyflavanone + [reduced NADPH-hemoprotein reductase] + O2
-
 Petunia x hybrida 5,7,3',4',5'-pentahydroxyflavanone + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.81 5,7,4'-trihydroxyflavanone + [reduced NADPH-hemoprotein reductase] + O2
-
 Petunia x hybrida 5,7,3',4'-tetrahydroxyflavanone + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.81 dihydroquercetin + [reduced NADPH-hemoprotein reductase] + O2
-
 Petunia x hybrida 5'-hydroxydihydroquercetin + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.81 naringenin + 2 [reduced NADPH-hemoprotein reductase] + 2 O2
-
 Petunia x hybrida (2S)-5,7,3',4',5'-pentahydroxyflavanone + 2 [oxidized NADPH-hemoprotein reductase] + 2 H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.14.81 F3',5'H
-
 Petunia x hybrida

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.14.81 8
-
-
 Petunia x hybrida

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.14.81 cytochrome P450 inhibition by a polyclonal antibody that inhibits higher plant NADPH-cytochrome p450 reductase is consistent with the suggestion that flavonoid 3',5'-hydroxylase is a monooxygenase consisting of cytochrome p450 and a NADPH-cytochrome P-450 reductase Petunia x hybrida