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Literature summary extracted from

  • Rognes, S.E.; Dewaele, E.; Aas, S.F.; Jacobs, M.; Frankard, V.
    Transcriptional and biochemical regulation of a novel Arabidopsis thaliana bifunctional aspartate kinase-homoserine dehydrogenase gene isolated by functional complementation of a yeast hom6 mutant (2003), Plant Mol. Biol., 51, 281-294.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.3 gene akthr2, DNA sequence determination and analysis, located on chromosome 4, subcloning in Escherichia coli strain DH5alpha, functional complementation of the Saccharomyces cerevisiae homoserine dehydrogenase-deficient hom6 mutant strain and of the aspartate kinase-deficient hom3 mutant strain, conferring L-threonine and L-methionine prototrophy to the yeast cells Arabidopsis thaliana
2.7.2.4 by functional complementation of a Saccharomyces cerevisiae strain mutated in its homoserine dehydrogenase gene (hom6), expression in Escherichia coli, two of the three isolated clones are also able to complement a mutant yeast aspartate kinase gene (hom3), expression of the AK-HSDH gene in Arabidopsis thaliana (meristematic cells, leaves and stamens) Arabidopsis thaliana

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.3 additional information construction of transgenic Arabidopsis thaliana plants by transformation with gene akthr2 via Agrobacterium tumefaciens infection, determination of expression patterns of the gene akthr1 ans akthr2 in the transgenic plants Arabidopsis thaliana
1.1.1.3 additional information mutant strain M20-20D is deficient in gene HOM6 and shows no activity, the defect can be complemented by recombinant expression of the Arabidopsis thaliana gene akthr2 in the mutant yeast cells Saccharomyces cerevisiae
2.7.2.4 S2207A phenotype Thr- Met- Ura- Arabidopsis thaliana
2.7.2.4 S2207A/pM1-1 mutant S2207A complemented with plasmid pM1-1, phenotype Thr+ Met+ Ura+ Arabidopsis thaliana

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.3 L-threonine
-
Arabidopsis thaliana
2.7.2.4 L-alanine no significant effect on AK activity at 5 and 20 mM Arabidopsis thaliana
2.7.2.4 L-isoleucine no significant effect on AK activity at 5 and 20 mM Arabidopsis thaliana
2.7.2.4 L-lysine no significant effect on AK activity at 5 and 20 mM Arabidopsis thaliana
2.7.2.4 L-threonine inhibition at 0.5-1.0 mM in complemented S2207A cells is variable, it does not exceed 67% Arabidopsis thaliana

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.2.4 chloroplast
-
Arabidopsis thaliana 9507
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.3 Mg2+ required for aspartate kinase activity Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.3 L-homoserine + NADP+ Saccharomyces cerevisiae
-
L-aspartate 4-semialdehyde + NADPH
-
?
1.1.1.3 L-homoserine + NADP+ Arabidopsis thaliana
-
L-aspartate 4-semialdehyde + NADPH
-
?
2.7.2.4 ATP + L-aspartate Arabidopsis thaliana
-
ADP + phospho-L-aspartate
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.3 Arabidopsis thaliana
-
var. Bensheim showing threonine and methionine prototrophy, bifunctional enzyme showing aspartate dehydrogenase and aspartate kinase activity, gene akthr2
-
1.1.1.3 Saccharomyces cerevisiae
-
mutant strain M20-20D and wild-type strain S2207A
-
2.7.2.4 Arabidopsis thaliana
-
-
-
2.7.2.4 Arabidopsis thaliana
-
Saccharomyces cerevisiae M20-20D cells are transformed with A. thaliana cDNA, protein expressed and purified from Saccharomyces cerevisiae, all data refer to the recombinant yeast protein
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.2.4
-
Arabidopsis thaliana

Reaction

EC Number Reaction Comment Organism Reaction ID
1.1.1.3 L-homoserine + NAD(P)+ = L-aspartate 4-semialdehyde + NAD(P)H + H+ bifunctional enzyme showing aspartate kinase, 2.7.2.4, and homoserine dehydrogenase activities Arabidopsis thaliana

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.3 leaf
-
Arabidopsis thaliana
-
1.1.1.3 meristem
-
Arabidopsis thaliana
-
1.1.1.3 additional information akthr2 is not or time-restricted expressed in stem, gynoecium, and during seed formation Arabidopsis thaliana
-
1.1.1.3 stamen
-
Arabidopsis thaliana
-
2.7.2.4 leaf
-
Arabidopsis thaliana
-
2.7.2.4 meristem
-
Arabidopsis thaliana
-
2.7.2.4 stamen
-
Arabidopsis thaliana
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.2.4 0.0013
-
mutant S2207A Arabidopsis thaliana
2.7.2.4 0.0107
-
complemented mutant A2207A/pM1-1 Arabidopsis thaliana
2.7.2.4 0.0268
-
wild type X-2180-1A Arabidopsis thaliana

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.3 L-homoserine + NADP+
-
Saccharomyces cerevisiae L-aspartate 4-semialdehyde + NADPH
-
?
1.1.1.3 L-homoserine + NADP+
-
Arabidopsis thaliana L-aspartate 4-semialdehyde + NADPH
-
?
1.1.1.3 L-homoserine + NADP+
-
Arabidopsis thaliana L-aspartate 4-semialdehyde + NADPH
-
r
2.7.2.4 ATP + L-aspartate
-
Arabidopsis thaliana ADP + phospho-L-aspartate
-
?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.3 AK-HSDH
-
Arabidopsis thaliana
1.1.1.3 aspartate kinase-homoserine dehydrogenase
-
Arabidopsis thaliana
1.1.1.3 HSDH
-
Saccharomyces cerevisiae
2.7.2.4 AK
-
Arabidopsis thaliana
2.7.2.4 aspartate kinase
-
Arabidopsis thaliana

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.3 NADP+
-
Saccharomyces cerevisiae
1.1.1.3 NADP+
-
Arabidopsis thaliana
1.1.1.3 NADPH
-
Arabidopsis thaliana