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Literature summary extracted from

  • Strassner, J.; Schaller, F.; Frick, U.B.; Howe, G.A.; Weiler, E.W.; Amrhein, N.; Macheroux, P.; Schaller, A.
    Characterization and cDNA-microarray expression analysis of 12-oxophytodienoate reductases reveals differential roles for octadecanoid biosynthesis in the local versus the systemic wound response (2002), Plant J., 32, 585-601.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.1.42 expression of His-tagged isozyme OPR1 in Escherichia coli, transient expression of isozyme OPR1 as enhanced-GFP- or RFP-fusion protein in onion epidermal cells for subcellular localization study Solanum lycopersicum
1.3.1.42 expression of His-tagged isozyme OPR2 in Escherichia coli Solanum lycopersicum
1.3.1.42 expression of His-tagged isozyme OPR3 in Escherichia coli, transient expression of isozyme OPR3 as enhanced-GFP- or RFP-fusion protein in onion epidermal cells for subcellular localization study Solanum lycopersicum
1.3.1.42 expression of the 3 isozymes OPR1-3 as N-terminally GST- or His-tagged enzymes in Escherichia coli Arabidopsis thaliana

Protein Variants

EC Number Protein Variants Comment Organism
1.3.1.42 additional information construction of isozyme OPR3 mutant plants for subcellular localization study of the isozyme, overview Arabidopsis thaliana

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.3.1.42 cytosol isozyme OPR1 Solanum lycopersicum 5829
-
1.3.1.42 cytosol isozyme OPR2 Solanum lycopersicum 5829
-
1.3.1.42 cytosol isozymes OPR1 and OPR2 Arabidopsis thaliana 5829
-
1.3.1.42 peroxisome isozyme OPR3 Arabidopsis thaliana 5777
-
1.3.1.42 peroxisome isozyme OPR3 Solanum lycopersicum 5777
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.3.1.42 Mg2+
-
 Arabidopsis thaliana
1.3.1.42 Mg2+
-
 Solanum lycopersicum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.1.42 (15Z)-12-oxophyto-10,15-dienoate + NADPH Arabidopsis thaliana
-
 8-[(1R,2R)-3-oxo-2-((Z)-pent-2-enyl)cyclopentyl]octanoate + NADP+
-
 ?
1.3.1.42 (15Z)-12-oxophyto-10,15-dienoate + NADPH Solanum lycopersicum
-
 8-[(1R,2R)-3-oxo-2-((Z)-pent-2-enyl)cyclopentyl]octanoate + NADP+
-
 ?
1.3.1.42 (9R,13R)-(15Z)-12-oxophyto-10,15-dienoate + NADPH Solanum lycopersicum
-
 (9R,13R)-8-[(1R,2R)-3-oxo-2-((Z)-pent-2-enyl)cyclopentyl]octanoate + NADP+
-
 ?
1.3.1.42 (9S,13S)-(15Z)-12-oxophyto-10,15-dienoate + NADPH Solanum lycopersicum product is the direct precursor in the jasmonate biosynthesis (9S,13S)-8-[(1R,2R)-3-oxo-2-((Z)-pent-2-enyl)cyclopentyl]octanoate + NADP+
-
 ?
1.3.1.42 additional information Solanum lycopersicum phylogenetic analysis ?
-
 ?
1.3.1.42 additional information Solanum lycopersicum phylogenetic analysis, enzyme is involved in octadecanoid biosynthesis which has differential roles for in the local versus the systemic wound response ?
-
 ?
1.3.1.42 additional information Arabidopsis thaliana phylogenetic analysis, isozyme OPR3 is induced in self-defense signalling, enzyme is involved in octadecanoid and jasmonate biosynthesis with differential roles for in the local versus the systemic wound response, cDNA micro array analysis ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.42 Arabidopsis thaliana
-
 ecotype Col-0, 3 isozymes OPR1-3
-
1.3.1.42 Solanum lycopersicum Q9FEW9 isozyme OPR3; 3 isozymes OPR1-3
-
1.3.1.42 Solanum lycopersicum Q9FEX0 isozyme OPR2, 12-oxophytodienoate-like protein; 3 isozymes OPR1-3, 12-oxophytodienoate-like protein
-
1.3.1.42 Solanum lycopersicum Q9XG54 isozyme OPR1; 3 isozymes OPR1-3
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.1.42 recombinant His-tagged isozyme OPR1 from Escherichia coli by nickel affinity chromatography Solanum lycopersicum
1.3.1.42 recombinant His-tagged isozyme OPR2 from Escherichia coli by nickel affinity chromatography Solanum lycopersicum
1.3.1.42 recombinant His-tagged isozyme OPR3 from Escherichia coli by nickel affinity chromatography Solanum lycopersicum
1.3.1.42 recombinant tagged isozymes OPR1-3 from Escherichia coli by affinity chromatography Arabidopsis thaliana

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.3.1.42 leaf isozyme OPR3, up-regulation in wounded leaves, low activity in unwounded, systemic leaves Arabidopsis thaliana
-
1.3.1.42 mesophyll
-
 Arabidopsis thaliana
-
1.3.1.42 shoot
-
 Arabidopsis thaliana
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.42 (15Z)-12-oxophyto-10,15-dienoate + NADPH
-
 Arabidopsis thaliana 8-[(1R,2R)-3-oxo-2-((Z)-pent-2-enyl)cyclopentyl]octanoate + NADP+
-
 ?
1.3.1.42 (15Z)-12-oxophyto-10,15-dienoate + NADPH
-
 Solanum lycopersicum 8-[(1R,2R)-3-oxo-2-((Z)-pent-2-enyl)cyclopentyl]octanoate + NADP+
-
 ?
1.3.1.42 (15Z)-12-oxophyto-10,15-dienoate + NADPH racemic mixture as substrate is accepted by isozyme OPR1 Arabidopsis thaliana 8-[(1R,2R)-3-oxo-2-((Z)-pent-2-enyl)cyclopentyl]octanoate + NADP+
-
 ?
1.3.1.42 (9R,13R)-(15Z)-12-oxophyto-10,15-dienoate + NADPH
-
 Solanum lycopersicum (9R,13R)-8-[(1R,2R)-3-oxo-2-((Z)-pent-2-enyl)cyclopentyl]octanoate + NADP+
-
 ?
1.3.1.42 (9R,13R)-(15Z)-12-oxophyto-10,15-dienoate + NADPH isozyme OPR1, no activity with the (9S,13S)-(15Z)-12-oxophyto-10,15-dienoate enantiomer Solanum lycopersicum (9R,13R)-8-[(1R,2R)-3-oxo-2-((Z)-pent-2-enyl)cyclopentyl]octanoate + NADP+
-
 ?
1.3.1.42 (9R,13R)-(15Z)-12-oxophyto-10,15-dienoate + NADPH isozymes OPR3 Solanum lycopersicum (9R,13R)-8-[(1R,2R)-3-oxo-2-((Z)-pent-2-enyl)cyclopentyl]octanoate + NADP+
-
 ?
1.3.1.42 (9S,13S)-(15Z)-12-oxophyto-10,15-dienoate + NADPH product is the direct precursor in the jasmonate biosynthesis Solanum lycopersicum (9S,13S)-8-[(1R,2R)-3-oxo-2-((Z)-pent-2-enyl)cyclopentyl]octanoate + NADP+
-
 ?
1.3.1.42 (9S,13S)-(15Z)-12-oxophyto-10,15-dienoate + NADPH isozyme OPR3 Solanum lycopersicum (9S,13S)-8-[(1R,2R)-3-oxo-2-((Z)-pent-2-enyl)cyclopentyl]octanoate + NADP+
-
 ?
1.3.1.42 additional information phylogenetic analysis Solanum lycopersicum ?
-
 ?
1.3.1.42 additional information phylogenetic analysis, enzyme is involved in octadecanoid biosynthesis which has differential roles for in the local versus the systemic wound response Solanum lycopersicum ?
-
 ?
1.3.1.42 additional information phylogenetic analysis, isozyme OPR3 is induced in self-defense signalling, enzyme is involved in octadecanoid and jasmonate biosynthesis with differential roles for in the local versus the systemic wound response, cDNA micro array analysis Arabidopsis thaliana ?
-
 ?
1.3.1.42 additional information no activity of isozyme OPR2 with (15Z)-12-oxophyto-10,15-dienoate stereoisomers Solanum lycopersicum ?
-
 ?
1.3.1.42 additional information stereospecificity of isozymes, overview, isozyme OPR1 shows a broad substrate specificity reducing a wide range of alpha,beta-unsaturated carbonyl compounds Arabidopsis thaliana ?
-
 ?
1.3.1.42 additional information stereospecificity of isozymes, overview, isozyme OPR1 shows a broad substrate specificity reducing a wide range of alpha,beta-unsaturated carbonyl compounds Solanum lycopersicum ?
-
 ?
1.3.1.42 additional information stereospecificity of isozymes, overview, isozyme OPR3 shows a broad substrate specificity reducing a wide range of alpha,beta-unsaturated carbonyl compounds Solanum lycopersicum ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.3.1.42 OPR
-
 Arabidopsis thaliana
1.3.1.42 OPR
-
 Solanum lycopersicum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.3.1.42 25
-
 assay at Arabidopsis thaliana
1.3.1.42 25
-
 assay at Solanum lycopersicum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.3.1.42 7.5
-
 assay at Arabidopsis thaliana
1.3.1.42 7.5
-
 assay at Solanum lycopersicum

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.1.42 flavin dependent on Arabidopsis thaliana
1.3.1.42 flavin dependent on Solanum lycopersicum
1.3.1.42 NADPH
-
 Arabidopsis thaliana
1.3.1.42 NADPH
-
 Solanum lycopersicum