Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Ishiga, Y.; Funato, A.; Tachiki, T.; Toyoda, K.; Shiraishi, T.; Yamada, T.; Ichinose, Y.
    Expression of the 12-oxophytodienoic acid 10,11-reductase gene in the compatible interaction between pea and fungal pathogen (2002), Plant Cell Physiol., 43, 1210-1220.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.3.1.42 additional information enzyme expression is inducible by regulatory suppressor glycopeptides from the fungus Mycosphaerella pinodes, which block the pea defense response induced by elicitors Pisum sativum

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.1.42 cDNA library construction, protein S64, DNA and amino acid sequence determination and analysis, expression in Escherichia coli strain BL21(DE3) as His-tagged protein Pisum sativum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3.1.42 0.043
-
(15Z)-12-oxophyto-10,15-dienoate pH 7.5, 25°C, recombinant enzyme Pisum sativum
1.3.1.42 0.055
-
2-cyclohexen-1-one pH 7.5, 25°C, recombinant enzyme Pisum sativum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.1.42 additional information Pisum sativum suppressor-inducible enzyme activity leads to jasmonate biosynthesis or related compound, which might contribute to compatibility by specific activation of the octadecanoid pathway and inhibition of the phenylpropanoid biosynthetic pathway ?
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.42 Pisum sativum Q9AVK9 cv. Midoriusui
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.3.1.42 epicotyl
-
Pisum sativum
-
1.3.1.42 leaf
-
Pisum sativum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.3.1.42 additional information
-
-
Pisum sativum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.42 (15Z)-12-oxophyto-10,15-dienoate + NADPH
-
Pisum sativum 8-[(1R,2R)-3-oxo-2-((Z)-pent-2-enyl)cyclopentyl]octanoate + NADP+
-
?
1.3.1.42 (15Z)-12-oxophyto-10,15-dienoate + NADPH + H+
-
Pisum sativum 8-[(1R,2R)-3-oxo-2-{(Z)-pent-2-enyl}cyclopentyl]octanoate + NADP+
-
r
1.3.1.42 2-cyclohexen-1-one
-
Pisum sativum ?
-
r
1.3.1.42 2-cyclohexen-1-one + NADPH best substrate Pisum sativum ? + NADP+
-
?
1.3.1.42 additional information suppressor-inducible enzyme activity leads to jasmonate biosynthesis or related compound, which might contribute to compatibility by specific activation of the octadecanoid pathway and inhibition of the phenylpropanoid biosynthetic pathway Pisum sativum ?
-
?

Synonyms

EC Number Synonyms Comment Organism
1.3.1.42 S64
-
Pisum sativum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.3.1.42 25
-
assay at Pisum sativum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.3.1.42 7.5
-
assay at Pisum sativum

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.1.42 NADPH
-
Pisum sativum