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Literature summary extracted from

  • Hakamatsuka, T.; Mori, K.; Ishida, S.; Ebizuka, Y.; Sankawa, U.
    Purification of 2-hydroxyisoflavanone dehydratase from the cell cultures of Pueraria lobata (1998), Phytochemistry, 49, 497-505.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.1.105 2,3-butadione 0.1 mM, complete inhibition Pueraria montana var. lobata
4.2.1.105 diethyldicarbonate 0.5 mM, 69% inhibition Pueraria montana var. lobata
4.2.1.105 iodoacetamide 50 mM, 56% inhibition Pueraria montana var. lobata
4.2.1.105 iodoacetic acid 50 mM, 43% inhibition Pueraria montana var. lobata
4.2.1.105 NEM 1.0 mM, 71% inhibition Pueraria montana var. lobata
4.2.1.105 PCMB 0.01 mM, 91% inhibition Pueraria montana var. lobata

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.1.105 7
-
 2,7,4'-trihydroxyisoflavanone
-
 Pueraria montana var. lobata

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.2.1.105 microsome
-
 Pueraria montana var. lobata
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.1.105 35000
-
 gel filtration Pueraria montana var. lobata
4.2.1.105 38000
-
 1 * 38000, SDS-PAGE Pueraria montana var. lobata

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.105 Pueraria montana var. lobata
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.105
-
 Pueraria montana var. lobata

Reaction

EC Number Reaction Comment Organism Reaction ID
4.2.1.105 2,4',7-trihydroxyisoflavanone = daidzein + H2O (1) Pueraria montana var. lobata
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.2.1.105 cell culture
-
 Pueraria montana var. lobata
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.1.105 3.408
-
-
 Pueraria montana var. lobata

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.105 2,7,4'-trihydroxyisoflavanone
-
 Pueraria montana var. lobata daidzein + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.1.105 monomer 1 * 38000, SDS-PAGE Pueraria montana var. lobata

Synonyms

EC Number Synonyms Comment Organism
4.2.1.105 2,7,4'-trihydroxyisoflavanone dehydratase
-
 Pueraria montana var. lobata

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.1.105 6.8
-
-
 Pueraria montana var. lobata

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.2.1.105 6 7.9 half-maximal activity at pH 6.0 and pH 7.9 in potassium phosphate buffer Pueraria montana var. lobata

Cofactor

EC Number Cofactor Comment Organism Structure
4.2.1.105 additional information no cofactor required Pueraria montana var. lobata

pI Value

EC Number Organism Comment pI Value Maximum pI Value
4.2.1.105 Pueraria montana var. lobata isoelectric focusing, pH gradient 4-6.5
-
 4.9